Recombinant Human GOLGA8CP Protein: Properties & Uses

Recombinant Human GOLGA8CP Protein is a research-grade recombinant protein derived from the human GOLGA8CP gene, belonging to the golgin family of coiled-coil proteins associated with Golgi apparatus structure and function.

Product Name

Recombinant Human GOLGA8CP Protein

SKU

rp372895

Protein Family

Golgin family (coiled-coil Golgi matrix proteins)

Species Origin

Human (Homo sapiens)

Application

Research use only; suitable for cell biology and Golgi apparatus studies

Format

Recombinant protein (expression system and tag details: see datasheet)

Introduction to GOLGA8CP and the Golgin Protein Family

GOLGA8CP (Golgin A8 Family Member C, Pseudogene) is a member of the golgin superfamily, a group of coiled-coil proteins that localize to the Golgi apparatus and play structural roles in maintaining Golgi morphology and facilitating vesicular transport. Golgins act as tethering factors that capture transport vesicles and help orchestrate the directionality of membrane trafficking within the secretory pathway.

The GOLGA8 subfamily is of particular interest due to its expansion in the human genome and its association with chromosomal regions implicated in copy number variation and genomic rearrangements. Recombinant expression of GOLGA8CP enables researchers to study its biochemical interactions and potential functional roles in vitro.

Biochemical Properties of Recombinant Human GOLGA8CP Protein

Recombinant Human GOLGA8CP Protein is produced using recombinant DNA technology to ensure a well-defined and reproducible protein product. As a golgin family member, GOLGA8CP is predicted to adopt extensive coiled-coil secondary structure, which is characteristic of proteins involved in Golgi tethering and scaffolding functions.

Key biochemical parameters such as molecular weight, purity grade, expression host, purification method, and storage conditions are detailed in the product datasheet available from Aladdin Scientific. Researchers are advised to consult the datasheet (SKU: rp372895) for lot-specific quality control data, including SDS-PAGE purity analysis and endotoxin levels, prior to experimental use.

Research Applications

Recombinant Human GOLGA8CP Protein is intended for research use and is suitable for a range of in vitro applications in cell biology and protein biochemistry, including:

• Protein–protein interaction studies: Investigating binding partners of GOLGA8CP within the Golgi matrix protein network using techniques such as co-immunoprecipitation or pull-down assays.
• Structural biology: Supporting biophysical characterization of coiled-coil domain architecture.
• Antibody development and validation: Serving as a positive control antigen for antibody-based detection assays (ELISA, Western blot).
• Functional genomics: Providing recombinant protein for rescue or overexpression experiments in cell lines where GOLGA8CP expression is perturbed.

All applications should be validated by the end-user under their specific experimental conditions.

Storage, Handling, and Quality Assurance

Proper storage and handling of recombinant proteins are critical to maintaining biological activity and ensuring experimental reproducibility. General best-practice recommendations for recombinant proteins include:

• Store at –20 °C or –80 °C as specified in the product datasheet; avoid repeated freeze–thaw cycles.
• Reconstitute lyophilized protein in the recommended buffer as described in the datasheet.
• Centrifuge briefly upon first use to collect all material from the tube walls.
• Aliquot into single-use volumes to minimize protein degradation.

Aladdin Scientific applies rigorous quality control procedures to each lot of Recombinant Human GOLGA8CP Protein (rp372895). Lot-specific certificates of analysis (CoA) are available upon request. For regulatory and safety information, consult the Safety Data Sheet (SDS) provided with the product.

Frequently asked questions

What is the gene background of GOLGA8CP and why is it classified as a pseudogene?

GOLGA8CP is annotated as a pseudogene member of the GOLGA8 subfamily of golgins. Pseudogenes are genomic sequences that resemble functional genes but may lack complete open reading frames or regulatory elements required for canonical protein expression. However, some pseudogene-derived transcripts and protein products have been detected and studied for potential biological roles. Researchers should consult current genome annotation databases (e.g., Ensembl, NCBI) for the latest functional status of GOLGA8CP.

What quality control tests are performed on Recombinant Human GOLGA8CP Protein (rp372895)?

Quality control procedures typically applied to recombinant proteins from Aladdin Scientific include SDS-PAGE analysis to assess purity, and may include endotoxin testing and identity confirmation by mass spectrometry or Western blot. Specific lot-level QC data are provided in the certificate of analysis (CoA). Please contact Aladdin Scientific or visit the product page for the most current lot-specific information.

Is Recombinant Human GOLGA8CP Protein (rp372895) suitable for in vivo or clinical use?

No. Recombinant Human GOLGA8CP Protein (rp372895) is sold for research use only and is not intended for diagnostic, therapeutic, or any in vivo human or veterinary use. Researchers must ensure compliance with all applicable institutional and regulatory requirements when using this reagent.

How should I cite or reference the use of this product in a publication?

When publishing results obtained using Recombinant Human GOLGA8CP Protein from Aladdin Scientific, include the product name, SKU (rp372895), supplier name (Aladdin Scientific), and lot number in the Materials and Methods section. This practice ensures experimental reproducibility and proper attribution.

References

1. Munro S. The golgin coiled-coil proteins of the Golgi apparatus. Cold Spring Harb Perspect Biol. 2011;3(6):a005256. doi:10.1101/cshperspect.a005256
2. Barr FA, Short B. Golgins in the structure and dynamics of the Golgi apparatus. Curr Opin Cell Biol. 2003;15(4):405-413. doi:10.1016/S0955-0674(03)00054-1
3. NCBI Gene entry for GOLGA8CP. National Center for Biotechnology Information. Accessed 2024.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.