Recombinant Human GPATCH2 Protein: Key FAQs

Recombinant Human GPATCH2 Protein is a recombinant form of the human G-patch domain-containing protein 2, produced for use in biochemical, structural, and functional research applications.

Product Name

Recombinant Human GPATCH2 Protein

SKU

rp368099

Protein Family

G-patch domain-containing proteins

Species

Human (Homo sapiens)

Expression System

Recombinant (specific host system to be confirmed with datasheet)

Primary Research Use

Biochemical assays, protein interaction studies, and RNA-related functional studies

What Is GPATCH2 and Why Is It Scientifically Relevant?

GPATCH2 (G-Patch Domain-Containing Protein 2) is a member of the G-patch domain protein family. The G-patch motif is a conserved glycine-rich domain found in various eukaryotic proteins and certain viral proteins. Proteins harboring this domain are generally implicated in RNA processing, pre-mRNA splicing, and ribonucleoprotein complex remodeling. GPATCH2 has been studied in the context of cellular RNA metabolism and has attracted interest for its potential roles in oncogenesis and gene regulation. Research into GPATCH2 may shed light on splicing regulation and related disease mechanisms, making the recombinant protein a valuable tool for in vitro studies.

What Are the Key Specifications of Recombinant Human GPATCH2 Protein (rp368099)?

Recombinant Human GPATCH2 Protein (SKU: rp368099) is produced using recombinant expression technology to deliver a well-characterized human GPATCH2 protein for research use. Detailed specifications — including molecular weight, tag information, purity grade, endotoxin levels, and formulation — are provided on the official product datasheet available from Aladdin Scientific. Researchers are advised to consult the datasheet prior to experimental use to confirm suitability for their specific assay conditions. Key quality attributes typically assessed for recombinant proteins include SDS-PAGE purity, biological activity, and endotoxin content.

Note: Specific numeric values for purity, concentration, and activity are confirmed on the product-specific Certificate of Analysis (CoA). Please request the CoA for your specific lot number.

How Should Recombinant Human GPATCH2 Protein Be Stored and Handled?

Proper storage and handling of recombinant proteins are critical to maintaining activity and integrity. General best-practice guidelines for recombinant proteins of this type include:

• Short-term storage: Store at 4 °C if the protein will be used within 1–2 weeks of receipt.
• Long-term storage: Store at −20 °C or −80 °C in appropriate aliquots to avoid repeated freeze-thaw cycles.
• Aliquoting: Upon first thaw, divide the protein into single-use aliquots to minimize degradation.
• Avoid: Repeated freeze-thaw cycles, which can reduce protein activity and lead to aggregation.
• Carrier protein: Addition of a carrier protein (e.g., 0.1% BSA) may help stabilize diluted protein solutions.

Always refer to the product-specific datasheet for the recommended storage buffer composition, temperature, and shelf life, as these parameters are confirmed per lot.

What Research Applications Is Recombinant Human GPATCH2 Protein Suitable For?

Recombinant Human GPATCH2 Protein is intended for research use only and is suitable for a range of in vitro biochemical and cell biology applications, including but not limited to:

• Protein–protein interaction studies: Investigating binding partners of GPATCH2 within RNA processing complexes.
• Antibody validation and immunoassay development: Use as a positive control antigen in Western blot, ELISA, or immunoprecipitation experiments.
• Structural studies: Supporting crystallography or cryo-EM studies to determine three-dimensional structure.
• Enzymatic and functional assays: Assessing the role of GPATCH2 in RNA helicase stimulation or splicing factor interactions.
• Drug discovery screening: Identifying small molecules that modulate GPATCH2 function.

This product is not intended for diagnostic or therapeutic use in humans or animals.

How to Order and What Support Is Available from Aladdin Scientific?

Recombinant Human GPATCH2 Protein (SKU: rp368099) can be ordered directly through the Aladdin Scientific website or by contacting our customer service team. The following support resources are available:

• Technical Support: Our scientific support team can assist with application recommendations, protocol optimization, and troubleshooting.
• Documentation: Product datasheets, Safety Data Sheets (SDS), and Certificates of Analysis (CoA) are available upon request or via the product page.
• Custom Requests: For custom formulations, bulk quantities, or special packaging requirements, please contact Aladdin Scientific directly.
• Returns and Refunds: Please refer to Aladdin Scientific's returns and refunds policy for eligibility criteria concerning recombinant protein products.

For the most up-to-date pricing, availability, and promotional offers, visit the product listing at aladdin-e.com or contact your regional sales representative.

Frequently asked questions

What is Recombinant Human GPATCH2 Protein and what does GPATCH2 do?

Recombinant Human GPATCH2 Protein is a recombinant form of human G-Patch Domain-Containing Protein 2 (GPATCH2), produced for research applications. GPATCH2 belongs to the G-patch domain protein family, which is broadly involved in RNA processing, pre-mRNA splicing regulation, and the remodeling of ribonucleoprotein complexes. It has been studied for potential roles in oncogenesis and RNA metabolism.

What is the SKU for Recombinant Human GPATCH2 Protein at Aladdin Scientific?

The SKU for Recombinant Human GPATCH2 Protein at Aladdin Scientific is rp368099. You can search for this SKU directly on the Aladdin Scientific website to access the product page, datasheet, and ordering information.

How should I store Recombinant Human GPATCH2 Protein to maintain its activity?

For short-term use (within 1–2 weeks), store the protein at 4 °C. For long-term storage, aliquot the protein and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles that can reduce activity and promote aggregation. Always consult the product-specific datasheet for the exact recommended storage conditions for your lot.

Is Recombinant Human GPATCH2 Protein suitable for use as an antibody validation control?

Yes, recombinant proteins such as rp368099 are commonly used as positive control antigens for antibody validation in assays including Western blot, ELISA, and immunoprecipitation. Researchers should verify that the protein's tag and epitope regions do not interfere with the antibody's recognition site before use.

Can Recombinant Human GPATCH2 Protein be used for drug discovery applications?

Recombinant Human GPATCH2 Protein can be used in in vitro drug discovery screening applications, such as identifying small molecules that modulate GPATCH2 function or disrupt its protein–protein interactions. This product is intended for research use only and is not approved for therapeutic or diagnostic applications.

Where can I find the Certificate of Analysis (CoA) and datasheet for rp368099?

The datasheet for Recombinant Human GPATCH2 Protein (rp368099) is available on the Aladdin Scientific product page. For the lot-specific Certificate of Analysis (CoA), please contact Aladdin Scientific customer service with your lot number, or check whether it is available for download via the product page on aladdin-e.com.

References

1. Aladdin Scientific. Recombinant Human GPATCH2 Protein (rp368099) Product Page. Aladdin-e.com.
2. Fouraux MA, et al. The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase. RNA. 2002. [G-patch domain biology reference - general textbook-level knowledge]
3. General recombinant protein storage and handling guidelines. Textbook-level knowledge on recombinant protein best practices.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.