Recombinant Human GPR137B Protein: FAQs & Key Facts

Recombinant Human GPR137B Protein is a recombinant form of the human G protein-coupled receptor 137B, produced for use in biochemical and cell-based research applications requiring high-purity, functionally relevant receptor protein.

Product Name

Recombinant Human GPR137B Protein

SKU

rp368081

Protein Family

G Protein-Coupled Receptor (GPCR) superfamily, GPR137 subfamily

Species Origin

Human (Homo sapiens)

Supplier Reference

absea#PQ-207

Intended Use

Research use only (RUO); not for diagnostic or therapeutic use

What Is GPR137B and Why Is It Scientifically Relevant?

GPR137B (G Protein-Coupled Receptor 137B) is a member of the GPR137 subfamily within the large GPCR superfamily. GPCRs represent the largest class of cell-surface receptors in the human genome and are involved in transducing extracellular signals into intracellular responses, regulating a vast array of physiological processes including cell proliferation, neuronal signaling, and immune function.

GPR137B is thought to be an intracellular GPCR-like protein, with expression reported in various human tissues. Research into orphan GPCRs such as GPR137B is of particular interest because identifying their endogenous ligands and downstream signaling pathways may reveal novel drug targets and illuminate unexplored aspects of cell biology.

What Are the Recommended Applications for This Recombinant Protein?

Recombinant Human GPR137B Protein (rp368081) is suitable for a range of in vitro research applications. Typical uses for recombinant GPCR-family proteins of this type include:

• Protein–protein interaction studies: Investigating binding partners and receptor complex formation.
• Antibody production and validation: Serving as an antigen for generating or validating GPR137B-specific antibodies.
• Western blot and ELISA standards: As a positive control or standard in immunoassays.
• Structural and biochemical assays: Enabling characterization of receptor properties under defined in vitro conditions.

Users should confirm specific suitability for their assay format and optimize conditions in their own laboratory setting. Consult Aladdin Scientific technical support for application-specific guidance.

How Should Recombinant Human GPR137B Protein Be Stored and Handled?

Proper storage and handling are critical to maintaining the activity and integrity of recombinant proteins. General best-practice guidelines for recombinant proteins of this class include:

• Short-term storage (up to 1 month): Store at 4 °C in the supplied buffer.
• Long-term storage: Aliquot and store at −80 °C to avoid repeated freeze-thaw cycles, which can degrade protein activity and cause aggregation.
• Avoid freeze-thaw cycles: Prepare single-use aliquots upon first receipt to preserve protein integrity.
• Carrier protein addition: If working at very low concentrations, consider adding a carrier protein (e.g., 0.1% BSA) to prevent adsorptive losses.

Note: Always refer to the specific product datasheet provided with your shipment (absea#PQ-207) for authoritative storage instructions, as conditions may vary by lot and formulation.

Quality, Purity, and What to Expect Upon Receipt

Recombinant Human GPR137B Protein supplied by Aladdin Scientific (rp368081) is manufactured under quality-controlled conditions. For recombinant proteins in this category, researchers should expect:

• Purity assessment: Typically evaluated by SDS-PAGE and/or HPLC; specific purity data will be provided on the certificate of analysis (CoA) included with each lot.
• Endotoxin levels: Controlled and reported on the CoA; low endotoxin is important for cell-based assays.
• Identity confirmation: Verified by mass spectrometry or Western blot using a GPR137B-specific antibody.
• Presentation: Supplied as a lyophilized powder or liquid solution (refer to the datasheet absea#PQ-207 for exact formulation details).

If you have quality concerns upon receipt, contact Aladdin Scientific customer service immediately with your lot number and order details.

Ordering and Technical Support for rp368081

Recombinant Human GPR137B Protein is available for order through Aladdin Scientific under SKU rp368081. When placing your order, please note:

• Confirm the required quantity and format (lyophilized vs. liquid) with the product page or your Aladdin Scientific sales representative.
• Bulk or custom quantities may be available — contact Aladdin Scientific for pricing and lead times.
• For technical questions regarding experimental design, reconstitution protocols, or application suitability, contact Aladdin Scientific technical support.
• Refer to the supplier reference absea#PQ-207 when requesting the product datasheet or certificates of analysis.

Aladdin Scientific is committed to supporting researchers with high-quality recombinant proteins and responsive customer service.

Frequently asked questions

What is GPR137B and what role does it play in cell biology?

GPR137B is a member of the GPR137 subfamily of G protein-coupled receptors. It is considered an orphan GPCR, meaning its endogenous ligand has not yet been definitively identified. It is believed to function as an intracellular receptor involved in lysosomal biology and cellular signaling, and represents an active area of research for understanding novel receptor functions and potential therapeutic targets.

Is Recombinant Human GPR137B Protein (rp368081) suitable for use in cell-based functional assays?

The suitability of this recombinant protein for cell-based functional assays depends on the specific assay design, as membrane-spanning GPCRs present unique challenges for recombinant expression and functional reconstitution. We recommend consulting the product datasheet (absea#PQ-207) and contacting Aladdin Scientific technical support to discuss your experimental requirements before use in functional cell-based assays.

How should I reconstitute the lyophilized Recombinant Human GPR137B Protein?

Reconstitution should be performed according to the instructions provided in the product datasheet (absea#PQ-207). As a general guideline for recombinant proteins, briefly centrifuge the vial before opening, then reconstitute in the recommended volume of sterile, deionized water or the specified buffer. Mix gently and allow the solution to equilibrate before use. Avoid vigorous vortexing to prevent protein denaturation.

What quality documentation is available for rp368081?

Each lot of Recombinant Human GPR137B Protein is supplied with a Certificate of Analysis (CoA) that includes purity data, endotoxin levels, and identity confirmation results. For additional documentation such as Safety Data Sheets (SDS) or product datasheets, please contact Aladdin Scientific customer service and reference supplier code absea#PQ-207.

Can this product be used for in vivo animal studies?

Recombinant Human GPR137B Protein (rp368081) is supplied for research use only (RUO) and is not validated or approved for in vivo diagnostic or therapeutic use. Any in vivo research application should be evaluated carefully by the principal investigator according to institutional and regulatory requirements. Contact Aladdin Scientific technical support for further guidance.

References

1. Gantseva AR, et al. GPR137 family members: structure, function, and disease associations. [General review of GPR137 family — specific citation details require verification.]
2. Fredriksson R, Lagerstrom MC, Lundin LG, Schioth HB. The G-protein-coupled receptors in the human genome form five main families. Mol Pharmacol. 2003;63(6):1256–1272. doi:10.1124/mol.63.6.1256
3. Aladdin Scientific. Product Datasheet: Recombinant Human GPR137B Protein (rp368081), Supplier Reference absea#PQ-207. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.