Flow cytometry enables the separation of a suspended sample of cells or particles through a thin, rapidly moving liquid stream. As the sample passes through a laser, it can detect the size, granularity, and fluorescent properties of each individual cell or particle. This technique allows for the ...
Since the first publication describing Western blotting, this immunodetection technique has been widely used to identify specific proteins in complex mixtures extracted from cells or tissues.
Quantum dots are tiny nanocrystals that glow when stimulated by an external light source, such as ultraviolet light. How many atoms are contained in the dots determines their size, and the size of the dots determines the color of the light emitted.
Antibodies, also referred to as immunoglobulins (Ig), are significant Y-shaped glycoproteins produced by B-cells as a primary immune defense. These large proteins specially bind unique pathogen molecules that are known as antigens.
The absorption of light results in dye molecules entering an electronically excited state, which can store energy only for a brief period and can be radiated again during fluorescence after the excited state's lifetime.
Thiazole, also known as 1,3-thiazole, is a five-membered heterocyclic compound containing both sulfur and nitrogen. The term "thiazole" also refers to a large group of derivatives. Thiazole itself is a pale yellow liquid with a pyridine-like odor and the molecular formula C3H3NS.[1,2] The ...
The fluorescence of a compound can be quenched, meaning it is reduced or completely switched off by processes that do not destroy the fluorophore permanently. Fluorescence quenching reduces the intensity of fluorescence, but the original intensity reappears as soon as the quencher is removed or ...
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