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Laboratory Protocols & SOPs Library

Tris-Buffered Saline (TBS)

· Published 27/07/2023 · Updated 09/06/2025

Recipe

1.Dissolve the following in 800ml of distilled H2O.

8g of NaCl, 0.2g of KCl
3g of Tris base

2.Add 0.015g of phenol red.

3.Adjust the pH to 7.4 with HCl.

4.Add distilled H2O to 1L.

5.Sterilize by autoclaving.

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Tris-Buffered Saline (TBS)" Aladdin Knowledge Base, updated 09/06/2025. https://www.aladdinsci.com/eu_pt/faqs/tris-buffered-saline-tbs-en.html

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