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Laboratory Protocols & SOPs Library

Trypan Blue

· Published 27/07/2023 · Updated 09/06/2025

Recipe

1.Dissolve the following in 80ml PBS

0.4g Trypan blue

2.Bring to a slow boil

3.Cool to room temperature

4.qs to 100ml with PBS

Add 0.1 volumes of trypan blue solution to 0.9 volumes of cells in PBS. Unstained cells are viable.

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Trypan Blue" Aladdin Knowledge Base, updated 09/06/2025. https://www.aladdinsci.com/eu_pt/faqs/trypan-blue-en.html

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