GRAU & PUREZABioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Biological Stain?Biological stain grade — dyes characterized for staining cells and tissues. Use in histology and microscopy where staining consistency matters.for Fluorescence analysis?Fluorescence-analysis grade — very low fluorescent impurities for clean spectra. Use in fluorescence assays where background interferes.for Microscopy?Microscopy grade — reagents/stains suited to sample prep and imaging. Use in microscopy where clarity and low background are needed.≥95%(HPLC)
Synonyms
Fluorescent Probe for Intracellular L Fe²⁺ Fluorescent Probe bile Fe²⁺ | Intracellular Labile Fe²⁺ Fluorescent Probe | Fe2+ indicator
BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥95%(HPLC) Biological Stain,BioReagent,for Fluorescence analysis,for Microscopy para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.
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Armazenamento e envio
Protected from light,Store at -20°C Envio Ice chest + Ice pads. Consulte o COA específico do lote para obter as especificações exatas.
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Documentos de qualidade
SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.
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Comprovação documental
Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.
Visão geral
Iron is the most abundant transition metal element in organisms and participates in a wide range of physiological processes. In recent years, labile intracellular iron has attracted extensive research attention. Labile iron primarily exists in two stable redox states: ferrous ion (Fe²⁺) and ferric ion (Fe³⁺). For researchers investigating the intracellular reductive environment, metal transporters and the aqueous solubility of Fe²⁺, characterizing the behavior of Fe²⁺ is more critical than studying Fe³⁺. As a novel fluorescent probe, RhoNox-4 enables fluorescence imaging of intracellular Fe²⁺ in live cells. The fluorescence intensity enhancement induced by the reaction between RhoNox-4 and Fe²⁺ is irreversible, which distinguishes it from calcium-sensing fluorescent probes such as Fluo-3 (Cat.No.: F131394) that support real-time monitoring of calcium ions.
Especificações
Sinónimos
Fluorescent Probe for Intracellular L Fe²⁺ Fluorescent Probe bile Fe²⁺ | Intracellular Labile Fe²⁺ Fluorescent Probe | Fe2+ indicator
Especificações e pureza
BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥95%(HPLC)
Estabilidade e armazenamento
Store at -20℃ long term (12 months). Store in the dark.
Condições de armazenamento de armazenamento
Protected from light,Store at -20°C
Enviado em
Ice chest + Ice pads
Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.
Grau
Biological Stain, BioReagent, for Fluorescence analysis, for Microscopy
Flow cytometric histograms of Jurkat cells stained with FerroOrange.
Black: untreated cells without probe (negative control);
Blue: untreated cells with probe (basal Fe²⁺ level);
Red: cells pretreated with 100 μM ferrous ammonium sulfate (FAS) (A112646) for 30 min before probe incubation (elevated Fe²⁺);
Green: cells pretreated with 100 μM 2,2'-bipyridyl (BPY) (D108977) for 30 min before probe incubation (Fe²⁺ chelation).
RhoNox-4/FerroOrange (Fe²⁺ Fluorescent Probe) (F1455371) - IF
Confocal fluorescence images of Jurkat cells stained with the Fe²⁺ probe FerroOrange (RhoNox-4).
Left: untreated cells show weak red fluorescence, indicating endogenous Fe²⁺.
Middle: after treatment with 100 μM ferrous ammonium sulfate (FAS) (A112646) for 30 min, the red fluorescence intensity increases significantly, reflecting elevated intracellular Fe²⁺.
Right: addition of 100 μM 2,2'-bipyridyl (BPY) (D108977), a Fe²⁺ chelator, binds Fe²⁺ and prevents its reaction with FerroOrange, resulting in a marked decrease in red signal.
Nomes e identificadores
Peso molecular
684.79
Documentação
📋 Ficha de dados de segurança (FDS)
Documento abrangente sobre perigos, manuseamento, armazenamento e conformidade regulamentar.
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