Halophilic Bacteria Selective Agar - BioReagent

Cat. No.: H1528298

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Visão geral

Contains 4% NaCl, for purification of Vibrio parahaemolyticus

Specifications

Especificações e pureza

BioReagent

Condições de armazenamento de armazenamento

Room temperature

Enviado em

Normal

Grau

BioReagent

Product Line

Produtos biológicos

Application Protocols

General protocol (literature; adapt to your SOP; not product specifications):

• Reconstitution

  • Weigh the recommended mass per liter (refer to product label/CoA).
  • Add to purified water with stirring; heat to dissolve completely.
  • If required, adjust pH to target range (often near neutral for halophiles) before sterilization.

• Sterilization and Pouring

  • Autoclave at 121°C for 15 minutes (liquid cycle).
  • Cool to 50–55°C; add sterile supplements if used (e.g., additional salts, trace elements, or antibiotics—if your method requires them). Mix gently to avoid bubbles.
  • Pour into sterile Petri dishes (typically 20–25 mL per 90-mm plate). Allow to solidify with lids ajar in a laminar-flow hood to minimize condensation.

• Inoculation and Incubation

  • Use saline-compatible diluents for sample prep to prevent osmotic shock.
  • Streak or spread appropriate volumes. Incubate inverted plates at temperature suitable for target halophiles (commonly 25–37°C; adjust as needed) for 24–72 h or longer depending on organism growth rates.

• Interpretation and QC

  • Include a positive-control halophile strain and a non-halotolerant control to verify selectivity.
  • Record colony morphology, pigmentation (some halophiles produce characteristic colors), and growth vs. controls.

Dispose of used plates as biohazard following autoclaving.

Biological Roles

This product itself has no biological role; it functions as a selective growth medium for halophilic and halotolerant bacteria.

Context on halophiles (general, literature):

• Halophilic bacteria are adapted to high-salt environments via osmoadaptation strategies:
  • “Salt-in” strategy: Intracellular accumulation of K+ and Cl− to balance external NaCl.
  • Compatible solutes: Synthesis/uptake of osmoprotectants (e.g., ectoine, betaine, trehalose) to maintain protein stability without high internal ionic strength.
• Selective media recreate environmental ionic conditions to support growth of halophiles while excluding non-halotolerant microbes.
• Some halophiles require Mg2+ and K+ in addition to NaCl; certain taxa prefer specific carbon/nitrogen sources. Exact nutrient composition of this item is not specified; tailor supplements as needed for your target organisms.

Laboratory implications:

• Use saline diluents for sample handling to avoid osmotic shock to halophiles.
• Incubation parameters (temperature, oxygen availability, salinity) strongly influence community composition recovered on plates.
• For downstream molecular work, high-salt carryover can inhibit enzymes; wash colonies in appropriate low-salt buffers gradually (step-down) or use salt-tolerant extraction protocols.

Buffer Applications

This product is not a dedicated buffering reagent. Any buffering capacity arises from components within the medium and varies by formulation.

• Buffer relevance (general, literature): Many complex media include phosphate or other buffering systems targeting near-neutral pH to support growth during incubation. The specific buffer system and target pH for this item are not specified; refer to CoA.
• Practical guidance:
  • If your protocol requires a defined pH window, confirm pH after reconstitution and post-autoclave. Adjust with sterile acid/base as needed.
  • For experiments probing pH–salt interactions on halophile growth, consider defined media where buffer concentration and identity (e.g., HEPES, MOPS, phosphate) can be precisely controlled.

For electrophoresis or cell culture buffer uses, a selective agar medium is not appropriate; use dedicated buffer formulations.

Green Alternatives

While culture media are not typically framed in a solvent replacement context, environmentally responsible practice can significantly reduce the footprint of agar-based workflows.

Greener practice options (general):

• Energy efficiency: Batch-prepare media and optimize autoclave loading to maximize throughput per cycle. Use validated liquid cycles to avoid oversterilization.
• Waste minimization: Pour only the volume needed; store unused sterile medium in sealed bottles for later use. Consolidate biohazard waste and autoclave at appropriate, not excessive, times.
• Water stewardship: Use appropriately purified water grades to avoid repeat preparations. Capture cooling water condensate where safe and feasible.
• Materials: Prefer reusable glassware (bottles, flasks, Petri dish alternatives such as glass plates) when biosafety and workflow allow, reducing single-use plastics.
• Additives: Favor salt/osmotic selectivity (hallmark of halophilic agar) over antibiotic supplements when scientifically acceptable, reducing antibiotic discharge risks.

Comparison table (general, literature):

• Conventional selective agar with antibiotics vs. halophile-selective high-salt agar:
  • Mechanism: Chemical inhibition vs. osmotic selectivity
  • Environmental concern: Antibiotic resistance pressure vs. low
  • Trade-off: Broader inhibition spectrum vs. requires careful salt control

Note: These are general sustainability suggestions; align with institutional EHS policies.

Pharmaceutical Uses

This product is intended for research use only (from Product Data). It is not classified as a pharmaceutical excipient or active ingredient.

Relevant contexts (non-clinical, general):

• Environmental monitoring or bioburden investigations in saline process streams within biotech or industrial settings where halotolerant contaminants may be of interest.
• Method development for selective recovery of halophiles associated with raw materials (e.g., saline solutions) in research laboratories.

Compliance note:

• No pharmacopeial status or monograph is indicated for this item. For regulated applications, validated, compendial media should be selected where required and lot qualification performed per QA procedures.

No clinical diagnostic or therapeutic claims are made; laboratory research use only.

Physical Properties

As a dehydrated culture medium blend, conventional single-compound physical constants do not apply. Item-specific values not listed below should be obtained from the CoA/Spec Sheet.

• Appearance (dehydrated): Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Freely dispersible in water upon heating (general, literature). Final clarity/opalescence depends on formulation and salinity.
• pH (at working concentration): Not specified for this item; refer to CoA/Spec Sheet. Typical halophile media target pH ~7.0–7.5 (literature, varies by taxa and medium design).
• Density, refractive index, melting/boiling points: Not applicable to the dehydrated blend; the prepared medium is an aqueous gel once cooled.
• Osmolality/ionic strength: Determined by salt content; halophilic selective media commonly employ elevated NaCl (e.g., 5–20% w/v) or complex brines (literature). Exact values for this item are not specified.

Preparation-related properties (literature):

• Agar gelation typically occurs at ~32–40°C and melts at ~85–95°C (for standard agar; literature values), enabling pour-plate preparation post-sterilization.

Important: Do not treat literature values as product specifications. Where control of pH, salinity, or clarity is critical, adjust and verify after reconstitution according to your application and the CoA.

Quality and Grades

• Item-specific grade: BioReagent (from Product Data).

What BioReagent typically implies (general context):

• Prepared and quality-checked to meet common laboratory research requirements for biological experiments (microbiology, molecular biology support), focusing on functional performance (growth/selectivity) rather than ultra-trace analytical purity.
• Batch-to-batch QC often includes growth promotion, selectivity/indicative organisms, pH after reconstitution, and gel strength (for agar-based media). Specific QC metrics for this item are not specified; refer to CoA.

How to use grade information in practice:

• Suitable for research use (microbial isolation/enumeration, environmental/industrial sample testing). Not for clinical diagnostics or therapeutic use.
• For analytical chromatography or LC–MS workflows, this medium is not intended as a solvent/reagent; any extraction from plates should consider background matrix effects.

Stabilizers/antimicrobials:

• None specified for this item; refer to CoA/Spec Sheet. Many selective agars rely on ionic/osmotic selectivity rather than antibiotic supplements; if your protocol uses antibiotic additives, sterilize and add aseptically post-autoclave at ~50–55°C.

Documentation tip:

• Request the lot-specific CoA for pH (reconstituted), recommended dosage (g/L), selectivity controls, and performance acceptance criteria.

Reaction and Applications

Chemical reaction use is not applicable. Instead, the relevant context is microbiological application.

Research and testing applications (general, literature-based):

• Selective isolation and enumeration of halophilic and halotolerant bacteria from:
  • Marine and hypersaline environments (seawater, salt lakes, solar salterns)
  • Industrial brines and process waters (pickling, tanning, petroleum-associated brines)
  • Salted foods and curing brines (quality monitoring)
• Enrichment prior to downstream analyses (16S rRNA sequencing, metagenomics, biochemical profiling) under high-salt culture conditions.
• Phenotypic assessment of salt tolerance ranges by preparing plates with graded salinity (e.g., 5–25% NaCl, literature). This item’s exact salt level is not specified; if needed, prepare custom gradients with appropriate sterile supplements.

Practical tips (general):

• Pre-warm high-salt media to avoid premature gelling during pouring; pour at ~50–55°C to minimize condensation and ensure homogeneous salt distribution.
• Incubate at temperatures appropriate for target halophiles (often 25–37°C; extremophiles may require different temperatures; literature guidance only).
• For colony recovery, avoid excessive dilution into low-salt buffers; use saline-based diluents to prevent osmotic shock.

Note: Manufacturer-specific application statements are not provided for this item.

Reaction Conditions

Interpreted as preparation/use conditions for the medium (general guidance; not product specifications). Always defer to the product label/CoA for exact instructions.

Typical preparation (literature):

• Working concentration: Follow label grams per liter; when unspecified, many agar media are prepared at 35–75 g/L total medium to yield ~1.5% agar. This item’s exact dosage is not specified; refer to CoA.
• Dissolution: Suspend dehydrated powder in water with stirring; heat to near boiling until fully dissolved.
• Sterilization: Autoclave at 121°C for 15 minutes (liquid cycle). Avoid excessive times to preserve nutrients.
• Pouring: Cool to ~50–55°C, then pour plates. For selective high-salt media, maintain even temperature to prevent localized crystallization or premature gelling.
• Incubation: 25–37°C is common for many halophiles; adjust temperature and salinity to the target organism (literature). Aerobic incubation typical; some taxa require microaerophilic/anaerobic setups.

Quality checks (general):

• Verify pH and salinity after sterilization (conductivity/osmolality as needed).
• Include positive controls (known halophile) and negative controls (non-halotolerant strain) to confirm selectivity.

Note: If antibiotics or heat-labile supplements are used, sterile-filter and add aseptically after autoclaving once cooled to ~50–55°C.

Safety and Handling

• GHS classification, pictograms, signal word, and H-statements: Not specified for this item; refer to SDS.
• General hazards (literature/good laboratory practice):
  • Dehydrated culture media may generate dust; avoid inhalation. Use local exhaust or handle in a fume hood when weighing/dispensing.
  • High-salt formulations are hygroscopic; keep containers tightly closed to prevent clumping and composition drift.
  • After inoculation/usage, plates/broths should be considered biohazardous. Handle per institutional biosafety procedures (BSL appropriate to the organism), and autoclave before disposal.
• Incompatibilities (general): Avoid moisture uptake in storage; segregate from strong oxidizers until reconstituted. No special reactivity expected for the dry blend beyond normal organic/nutrient mix and salts.
• PPE: Laboratory coat, nitrile gloves, and eye protection. For large-scale media prep or dusty handling, consider a particulate respirator (P2/N95) per risk assessment.
• First aid (general guidance; defer to SDS):
  • Inhalation: Move to fresh air; seek medical advice if irritation persists.
  • Skin/eye contact: Rinse with water; remove contaminated clothing. Seek medical attention for persistent irritation.
  • Ingestion: Rinse mouth; do not induce vomiting; seek medical advice.

Always consult the product’s SDS for authoritative hazard and response information.

Solvent Selection

This product is a dehydrated culture medium intended to be reconstituted in purified water. Organic solvent selection is not applicable.

• Primary solvent: High-purity water (e.g., ASTM Type I/II or equivalent). Avoid high-TOC water that can alter selectivity or background.
• Ionic strength/salinity: Critical parameter for halophile selection. Adjust only as directed by the manufacturer’s formulation; excessive dilution or unintended salt additions can compromise selectivity.
• pH adjustment: If required by your method, adjust with sterile 1 N HCl/NaOH after dissolution and before sterilization. Verify pH after autoclaving, as heat can shift pH in phosphate-containing media (general literature note).
• Clarifying tips:
  • Heat with agitation to fully dissolve before sterilization; do not overheat to the point of caramelization of complex nutrients.
  • If precipitation occurs upon cooling at very high salinity, ensure complete dissolution before autoclaving and cool gradually.

Comparison (literature, general):

• Halophile-selective agars vs. nonselective nutrient agar: The former maintains elevated salt/osmolyte content to exclude non-halotolerant flora, improving recovery of halophiles from saline matrices (seawater, brines, salted foods).

Storage and Reconstitution

• Storage conditions (item-specific): Room temperature (from Product Data). Keep container tightly closed in a dry place to minimize moisture uptake. Protect from direct sunlight and heat sources.
• Shipping: Shipped under normal conditions (from Product Data).

Reconstitution (general guidance; not product specification):

• Use the mass per liter indicated on the product label/CoA. Disperse in purified water with stirring; heat until fully dissolved.
• If pH adjustment is required by your method, adjust prior to sterilization; verify pH after autoclaving.
• Autoclave at 121°C for 15 minutes. Cool to 50–55°C before pouring plates or adding heat-labile supplements.

Storage after preparation (general):

• Store solidified plates at 2–8°C in sealed sleeves or bags to limit desiccation; warm to room temperature and dry surface condensation before use. Use within 2–4 weeks for best performance (literature guidance).
• Store sterile molten medium at 45–50°C (water bath) for same-day use to prevent premature gelling; avoid repeated melting of agar as it may affect gel strength.

Note: Exact reconstitution mass, final pH, and salinity for this item are not specified; consult the lot-specific CoA/Spec Sheet.

Structure and Identity

This product is a complex, dehydrated culture medium formulated to selectively cultivate halophilic bacteria; it is a mixture rather than a single defined chemical entity.

• Item-specific identifiers:

  • SKU: H1528298
  • Product Name: Halophilic Bacteria Selective Agar
  • Grade: BioReagent (from Product Data)
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey: Not applicable (complex mixture)
  • SMILES: Not applicable (complex mixture)
  • Molecular Formula: Not applicable (mixture)
  • Molecular Weight: Not applicable (mixture)

• Structural features (general knowledge for such media):

  • Typically contains an agar gelling matrix and high-salt components (e.g., NaCl, Mg2+/K+ salts) to enforce osmotic selectivity for halophiles.
  • May include complex nitrogen sources (peptone/tryptone) and growth factors (yeast extract), plus trace minerals; exact composition varies by manufacturer and is not specified for this item.

• 2D “structure” description (general):

  • Not represented by a single molecular structure. Function arises from the combined osmotic strength (high ionic content), nutrient profile, and solidifying polysaccharide (agar) forming a porous gel supporting colony formation.

Notes: For precise formulation and any defined component percentages, consult the product’s CoA/Specification Sheet.

Synthetic Utility

Not applicable. Halophilic Bacteria Selective Agar is a complex microbiological growth medium and is not used as a reagent or building block in chemical synthesis.

For synthetic chemistry applications requiring high ionic strength or gel matrices, consider defined salts or purified agar/agarose grades rather than a selective microbiological medium.

Target Specificity

This section is typically applicable to antibodies or affinity reagents. It is not applicable to a microbiological growth medium. For microbial selectivity details, refer to the Reaction & Applications and Application Protocols sections.