The Human Dendritic Cell Cytokine Package contains IL-4 and GM-CSF, key components required for the culturing of murine dendritic cells. Recombinant murine IL-4 is an essential cytokine used in the differentiation and maturation of murine dendritic cell in vitro. The IL-4 protein, specifically recombinant IL-4, promotes the development of dendritic cells from precursor cells such as monocytes. Murine IL-4 acts synergistically with other factors, including GM-CSF (granulocyte-macrophage colony stimulating factor), to drive the differentiation process. Typically, monocytes are cultured in the presence of recombinant IL-4 and GM-CSF to yield immature dendritic cells, which can then be futher matured by additional stimuli.
The package contains the following two vials:
Recombinant Human IL-4 Protein（Cat. No. rp156008）
Animal-Free Recombinant Human GM-CSF Protein （Cat. No. rp186551）
IL-4 is a pleiotropic cytokine that regulates diverse T- and B-cell responses including cell proliferation, survival, and gene expression. Produced by mast cells, T cells, and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells.
Information
Name
| Species
| Expression System
| Tag
| Purity
| Activity
| Endotoxin
| Appearance
| Storage
| Reconstitution
|
Recombinant Human IL-4 Protein
| Human | E. coli | N-His | ≥95% (SDS-PAGE) | Recombinant Human IL‑4 Protein (rp156008) stimulates cell proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.31 ng/mL. | <1.0 EU/μg | Lyophilized | Lyophilized from 10mM PBS, pH 7.4 | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Animal-Free Recombinant Human GM-CSF Protein
| Human
| 293F | No tag | ≥95% (SDS-PAGE) | Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 90.7 pg/mL. | <0.1 EU/μg | Lyophilized | Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4. | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Notes:
1. This product is for research use only.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
