I-A/I-E Rat mAb (PE) - Primary antibody, specific to I-A/I-E, Rat IgG2b

Cat. No.: Ab190258

GRADE & PURITY ExactAb™, Validated, Ex:565nm, Em:575nm, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

M5/114

Conjugation

PE

Visão geral

Rat anti Mouse I-A/I-E Antibody, Monoclonal (M5/114), could be used for Flow.

Specifications

Product Name

I-A/I-E Rat mAb (PE) - Primary antibody, specific to I-A/I-E, Rat IgG2b

Sinónimos

IAb | Ia2 | Ia-2 | Abeta | H-2Ab | H2-Ab | Rmcs1 | I-Abeta | Eb | Ia4 | H2Eb | Ia-4 | H-2Eb | E-alpha-f | H-2 class II histocompatibility antigen, E-K alpha chain | H-2 class II histocompatibility antigen, E-U alpha chain | H-2E | H2-Ea | H-2Ea | H2-Ea-ps

Especificações e pureza

ExactAb™, Validated, Ex:565nm, Em:575nm, 0.5 mg/mL

Mecanismos bioquímicos e fisiológicos

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides

Espécie hospedeira

Rat

Especificidade

I-A/I-E

Imunogénio

Activated C57BL/6 mouse spleen cells

Controlo positivo

Flow: C57BL/6 mouse splenocytes.

Conjugação

PE

Excitação (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emissão (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Grau

Em:575nm, Ex:565nm, ExactAb™, Validated

Isótipo

Rat IgG2b

Tipo de corrente de luz

kappa

Método de purificação

Protein G purified

Concentração

0.5 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at 2-8°C,Protected from light,Do not freeze

Enviado em

Wet ice,Do not freeze

Estabilidade e armazenamento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imagens

Flow Cytometry: I-A/I-E Rat mAb (PE) (Ab190258)

I-A/I-E Rat mAb (PE) (Ab190258) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with I-A/I-E Rat mAb (PE) (Ab190258) at 0.012μg/test for 1 hour at 4°C. Left panel - Rat IgG Isotype Control Antibody (PE) (Ab180312).

Flow Cytometry: I-A/I-E Rat mAb (PE) (Ab190258)

I-A/I-E Rat mAb (PE) (Ab190258) - Flow Cytometry
Flow cytometry analysis of I-A/I-E expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with I-A/I-E Rat mAb (PE) (Ab190258) at 0.012μg/test for 1 hour at 4°C. Blue - Rat IgG Isotype Control Antibody (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicação

Aplicação

Dilution info

Flow Cytometry

≤0.1 μg/test, the suggested use of this reagent is ≤0.1 μg per million cells in 100 µL staining volume

Perguntas frequentes

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (I-A/I-E Rat mAb (PE)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.