GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.≥95%(SDS-PAGE)1.0 mg/mL
Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:18692776, PubMed:7636208).
Also plays an important role in neutrophil activation (PubMe
Espécie hospedeira
Mouse
Especificidade
Human IL8
Imunogénio
Recombinant protein corresponding to Human IL8
Conjugação
Unconjugated
Grau
Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Tipo de corrente de luz
kappa
SDS-PAGE
150 kDa
Método de purificação
Protein A purified
Pares emparelhados
Ab214004Z; IL-8 Mouse mAb（B05/6E2）；ELISA
Concentração
1.0 mg/mL
Armazenamento e envio
Condições de armazenamento de armazenamento
Store at -20°C,Avoid repeated freezing and thawing
Enviado em
Ice chest + Ice pads
Estabilidade e armazenamento
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imagens
Sandwich ELISA - IL-8 standard curve
Sandwich ELISA detection of Recombinant Human IL-8 Protein (rp170400) using IL-8 Mouse mAb (Ab305305) as capture antibody at concentration of 1.0 µg/mL. Biotinylated IL-8 Mouse mAb (Ab214004) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 50.0 pg/mL - 4000.0 pg/mL
Indirect ELISA - IL-8 standard curve
Immobilized Recombinant Human IL-8 Protein (rp170400) at 1.0 µg/mL overnight at 4℃. Coated protein probed with IL-8 Mouse mAb (Ab305305). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC₅₀: 36.4 ng/mL
Aplicação
Aplicação
Dilution info
ELISA
1.0 µg/mL as a Capture antibody in ELISA
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Revisões
Avaliações dos Clientes
Perguntas frequentes
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
What is the purity of this product, and how is it determined?
This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.
What does Animal Free mean?
Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
