ITM2B Mouse mAb - Primary antibody, specific to ITM2B

Cat. No.: Ab111398

GRAU & PUREZA See COA

Application

Flow Cytometry, IHC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG2a

Clone Number

OTI1C11

Conjugation

Unconjugated

Visão geral

Mouse anti Human ITM2B Antibody, Monoclonal (OTI1C11), could be used for WB, IHC, Flow Cytometry and so on.

Especificações

Product Name

ITM2B Mouse mAb - Primary antibody, specific to ITM2B

Sinónimos

ABRI antibody|ABri/ADan amyloid peptide antibody|BRI 2 antibody|BRI antibody|BRI2 antibody|BRICD 2B antibody|BRICD2B antibody|BRICHOS domain containing 2B antibody|E25B antibody|E3 16 antibody|E3-16 antibody|FBD antibody|imBRI2 antibody|Immature BRI2 antibody|Integral membrane protein 2B antibody|ITM 2B antibody|ITM2B antibody|Protein E25B antibody|RDGCA antibody|Transmembrane protein BRI antibody|ITM2B|BRI|FBD|ABRI|BRI2|E25B|RDGCA|imBRI2|BRICD2B

Especificações e pureza

See COA

Mecanismos bioquímicos e fisiológicos

Functions as a protease inhibitor. Plays a role in APP processing regulating the physiological production of the beta amyloid peptide. Restricts docking of gamma-secretase to APP and access of alpha- and beta-secretase to their cleavage APP sequence. Post

Espécie hospedeira

Mouse

Especificidade

ITM2B

Imunogénio

Recombinant protein of human ITM2B (AA 1-266).

Controlo positivo

WB: A549, MCF7 lysates. IHC: Human Kidney tissue. Flow Cytometry: HeLa cells.

Conjugação

Unconjugated

SDS-PAGE

150 kDa

Método de purificação

Protein A/G purified

Concentração

See COA

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Aplicação

Aplicação

Dilution info

WB

1/2000

Flow Cytometry

1/100

IHC

1/150

Alvos associados (humanos)

ITM2B Tbio Integral membrane protein 2B (0 Activities)

Discover Target: ITM2B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Item-specific tested applications, recommended dilutions, incubation times, and positive control samples are not provided for this product. Refer to the CoA/Spec Sheet for validated protocols when available.

No additional application data have been specified by the manufacturer for this SKU.

Biological Roles

Target: ITM2B (Integral Membrane Protein 2B; also known as BRI2).

General biology (literature; not item-specific):

• ITM2B is a type II single-pass transmembrane glycoprotein with a luminal/extracellular C-terminus and a short N-terminal cytoplasmic domain.
• It is broadly expressed and implicated in protein processing and trafficking within the secretory pathway. Proteolytic processing yields extracellular peptides, and the intact protein can modulate interactions at the cell surface.
• ITM2B has been reported to interact with other membrane and secretory pathway proteins, influencing their maturation and surface presentation (e.g., roles in modulating proteolytic processing cascades).
• Cellular localization: Predominantly Golgi, plasma membrane, and endosomal compartments (literature), consistent with its membrane topology and trafficking.
• Functional themes: Regulation of membrane protein maturation, vesicular transport dynamics, and extracellular peptide generation. These roles make ITM2B a useful marker/target for studying membrane protein biology and secretory pathway regulation in diverse cell types.

Note: The above describes general biological context for the antigen and does not imply any medical or clinical application. Researchers should verify isoform expression, post-translational modifications, and subcellular localization in their specific biological system.

Buffer Applications

Antibody performance is highly dependent on buffer composition. Item-specific buffer/formulation is not provided for this product; the following are general considerations for monoclonal antibodies:

• Storage/stock buffers (general): PBS or TBS at pH 7.2–7.6. For −20°C storage, 30–50% glycerol is commonly used to prevent freezing and aggregation. Carrier proteins (0.1–1% BSA) can stabilize dilute solutions and reduce adsorption to plastics.
• Working buffers (assay-dependent):
  • WB/IHC/IF: PBS or TBS with 0.05–0.1% Tween 20 (PBST/TBST) for washes; blocking with 3–5% BSA or species-matched serum to minimize non-specific binding.
  • IP/Co-IP: Non-denaturing buffers containing 0.1–1% mild detergents (e.g., NP-40, Triton X-100) with protease/phosphatase inhibitors; maintain isotonic salt (100–150 mM NaCl) to preserve complexes.
  • ELISA: Carbonate-bicarbonate buffer (pH 9.4–9.6) for coating; PBS/TBS with protein blocker for sample and wash steps.
• Filtration/sterility: For cell-associated assays or long-term storage without preservatives, use 0.22 μm filtration to maintain sterility.
• Avoid: Extreme pH, high concentrations of chaotropes, or organic solvents that can denature antibodies unless purposefully eluting from affinity matrices.

Item-specific buffer recipe and preservative content: Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

While antibodies do not have traditional solvent replacements, greener laboratory practices can reduce environmental impact without compromising performance.

• Buffer preparation: Use pre-weighed PBS/TBS tablets to minimize chemical waste and weighing errors. Prepare only the volumes needed.
• Preservatives: If sodium azide is used (not specified for this item), collect azide-containing waste separately to prevent formation of heavy-metal azides in plumbing. Consider short-term storage without azide when possible, maintaining sterility by filtration and cold storage.
• Detergents: Opt for biodegradable, non-ionic detergents at the lowest effective concentration during washing steps.
• Cold chain efficiency: Consolidate shipments and use validated, recyclable cold-pack systems to reduce packaging waste (this item ships on ice packs as noted).
• Reuse and reduction: Aliquot strategically to reduce freeze–thaw cycles and avoid unnecessary re-preparation of diluted working solutions.

Comparison (general):

• Sodium azide vs ProClin-type biocide: Azide is effective but hazardous to aquatic environments and incompatible with HRP assays; ProClin blends are effective at low ppm but may have sensitization concerns. Choose based on assay type, safety policies, and disposal capabilities.

Item-specific formulation and preservative content: Not specified for this item; refer to CoA/Spec Sheet.

Pharmaceutical Uses

This product is designated for research use only and is not manufactured under GMP for clinical use.

• Formulation/excipient status: Not applicable; no pharmacopeial status is claimed for this item.
• Potential roles in pharmaceutical R&D (general):
  • Analytical reagent for characterization of ITM2B expression in cell lines or tissue samples during target discovery and validation.
  • Tool reagent in bioassays, screening cascades, or mechanism-of-action studies involving ITM2B-related pathways.
  • Reagent for immunocapture in analytical workflows (e.g., LC–MS sample prep) where specific enrichment of ITM2B-containing complexes is desired.

Regulatory note:

• Not for human or animal therapeutic use. No claims are made regarding safety, efficacy, or diagnostic performance. For any regulated application, obtain appropriate GMP-grade materials and validated methods.

Physical Properties

Item-specific numeric specifications (concentration, isotype, buffer, extinction coefficient, etc.): Not specified for this item; refer to CoA/Spec Sheet.

General properties for monoclonal IgG antibodies (literature/general):

• Approximate molecular weight: ~150 kDa for intact IgG; Fab ~50 kDa; Fc ~50 kDa.
• Physical state: Typically supplied in aqueous buffer (e.g., PBS, TBS) as liquid or lyophilized; actual state for this item not specified.
• Solubility: Highly soluble in physiological buffers (e.g., PBS, TBS). Denatured/aggregated forms may show reduced solubility.
• Isoelectric point (pI): Broadly ranges ~6–9 depending on isotype/sequence.
• Stability: Enhanced at lower temperatures and in presence of stabilizers (e.g., 10–50% glycerol, carrier proteins) under non-freezing conditions (general guidance).
• UV absorbance: Proteins exhibit A280 due to Trp/Tyr; for IgG, an approximate mass extinction coefficient is ε0.1% ≈ 1.36 at 280 nm (literature estimate); item-specific A280 factor not specified.
• Viscosity: Concentrated antibody solutions (>10 mg/mL) can be moderately viscous; dilute with compatible buffer to working concentration.

Thermal/chemical sensitivity (general):

• Repeated freeze–thaw cycles can promote aggregation and activity loss; store as aliquots (see Storage & Reconstitution).

Quality and Grades

Item-specific grade/purity, isotype, clonality details, endotoxin level, host species cross-reactivity, and formulation: Not specified for this item; refer to CoA/Spec Sheet.

General context for research-grade monoclonal antibodies:

• Research-use-only (RUO) designation: Suitable for laboratory research and method development. Not intended for diagnostic or therapeutic use.
• Typical characterization for high-quality mAbs (general expectations; not item-specific):
  • Verified specificity against the intended antigen by one or more applications (e.g., WB, IHC, IF, ELISA) and by knockdown/overexpression controls when available.
  • Isotype determination (e.g., mouse IgG1/IgG2a/IgG2b/IgG3, κ or λ light chain) to guide secondary antibody selection.
  • Formulation details (buffer composition, carrier proteins, preservatives) to inform storage and application compatibility.
  • Lot-to-lot verification and provision of a Certificate of Analysis (CoA) with performance criteria.

Implications for experimental design:

• Without item-specific details, users should validate working dilutions and assay conditions empirically and consult the CoA/SDS prior to use.
• If low background is critical (e.g., IHC), consider isotype- and species-matched negative controls; confirm that secondaries are anti-mouse and cross-adsorbed if needed.

Documentation: For final assay setup, rely on the lot-specific CoA/Spec Sheet for concentration (mg/mL), recommended dilutions, storage buffer, and stabilizers.

Reaction and Applications

Chemical reaction content is not directly applicable to antibodies. Instead, typical applications for monoclonal antibodies targeting ITM2B include immunochemical detection and enrichment. Manufacturer-provided tested applications are not specified for this item; the following are general research uses:

• Western blotting (WB): Detection of ITM2B in denatured lysates following SDS-PAGE. Antigen retrieval is not required; reducing vs non-reducing conditions may alter epitope exposure.
• Immunohistochemistry (IHC) and Immunofluorescence (IF): Localization of endogenous ITM2B in fixed tissues/cells. Optimize fixation (e.g., PFA vs methanol) depending on epitope sensitivity; antigen retrieval may be beneficial for formalin-fixed paraffin-embedded (FFPE) tissues.
• Immunoprecipitation (IP)/Co-IP: Enrichment of ITM2B and potential interaction partners from cell lysates under native or mild detergent conditions.
• ELISA: Quantitative assays for ITM2B or antibody titer assessment; confirm coating and blocking conditions.

Practical tips (general):

• Validate specificity with appropriate controls (knockout/knockdown lines or peptide-blocking where available).
• Titrate primary antibody to minimize background; optimize blocking (e.g., 3–5% BSA or serum) and washing stringency.
• Ensure secondary antibody species and isotype compatibility (anti-mouse secondary, cross-adsorbed if needed to reduce species cross-reactivity).

Item-specific tested applications/validated dilutions: Not specified for this item; consult the CoA/Spec Sheet.

Reaction Conditions

Classical chemical reaction conditions do not apply to antibodies. For immunochemical applications, general experimental conditions include:

• Temperature: Perform binding incubations at room temperature or 4°C depending on assay and affinity; lower temperatures can reduce background in IP/IF.
• Time: Primary antibody incubations often range from 1–2 h at room temperature to overnight at 4°C; secondary incubations are commonly 30–60 min (general guidance).
• Buffers: Isotonic buffers (PBS/TBS) with appropriate blockers and detergents (e.g., 0.05–0.1% Tween 20) to minimize non-specific binding.
• Detergents/denaturants: For WB under denaturing conditions, SDS-PAGE followed by transfer; for native IP, use mild detergents (0.1–1%) and avoid harsh denaturants.
• Antigen retrieval (IHC-FFPE): Heat-induced epitope retrieval (HIER) in citrate (pH ~6) or Tris-EDTA (pH ~9) buffers may improve signal for certain epitopes (optimize empirically).

Expected performance parameters, recommended dilutions, and tested conditions for this specific item: Not specified for this item; consult CoA/Spec Sheet and optimize empirically with appropriate controls.

Safety and Handling

Hazard classification (GHS): Not specified for this item; refer to SDS for authoritative safety data.

• Signal word: Not specified.
• H-statements/Pictograms: Not specified.

General laboratory safety for protein antibodies:

• Handle using good laboratory practice (GLP) with appropriate PPE: lab coat, gloves, and safety glasses.
• Avoid ingestion, inhalation of aerosols, and contact with skin/eyes. Work in a clean environment to prevent contamination of the reagent and exposure to biological materials.
• If the product contains preservatives such as sodium azide or ProClin (not specified for this item), consult the SDS for additional precautions, including aquatic toxicity and compatibility with metals.

First-aid (general guidance; defer to SDS):

• Skin/eye contact: Rinse with water for at least 15 minutes. Remove contaminated clothing. Seek medical attention if irritation persists.
• Inhalation: Move to fresh air; seek medical advice if symptoms occur.
• Ingestion: Rinse mouth; seek medical advice.

Incompatibilities and special risks (general):

• Avoid microbial contamination; use sterile technique if sterility is required for your workflow.
• Do not mix with incompatible buffers or extreme pH conditions that may denature proteins (e.g., <pH 4 or >pH 9 for prolonged periods).
• Protect from repeated freeze–thaw cycles to minimize aggregation and loss of binding activity.

Waste disposal: Follow institutional and local regulations for disposal of proteinaceous laboratory waste and any preservatives indicated on the SDS.

Solvent Selection

This product is a protein biologic (antibody) and is used in aqueous buffers rather than organic solvents.

General buffer/solvent considerations for antibodies (literature/best practice):

• Primary media: Isotonic, neutral pH buffers such as PBS (pH 7.2–7.6) or TBS (pH 7.4–7.6). Avoid extreme pH to preserve conformation.
• Additives: Glycerol (10–50%) for freeze protection at −20°C; carrier proteins (e.g., 0.1–1% BSA) to minimize adsorption in dilute solutions; non-ionic detergents (e.g., 0.01% Tween 20) to reduce nonspecific binding in some immunoassays.
• Sterility: Use sterile, filtered buffers for cell-related assays. For long-term storage, 0.02–0.05% sodium azide is commonly used as a preservative (note incompatibility with live cells and peroxidase reactions).
• Organic solvents: Generally avoided as they can denature antibodies; minimal percentages (<10%) of compatible cosolvents may be tolerated in some protocols, but confirm compatibility empirically.

When to choose PBS vs TBS (general):

• PBS: Widely used, biologically compatible; phosphate can interfere with certain metal-dependent interactions.
• TBS: Preferred when phosphate-sensitive interactions or detection chemistries are used.

Item-specific formulation/solvent details: Not specified for this item; refer to CoA/Spec Sheet.

Storage and Reconstitution

Item-specific storage/shipping (from Product Data):

• Storage: Store at −20°C; avoid repeated freezing and thawing.
• Shipping: Shipped in an ice chest with ice pads to maintain the cold chain.

General best practices (not item-specific):

• Upon receipt: Inspect for integrity of cold chain. If lyophilized, allow the vial to warm to room temperature in a desiccated environment before opening to prevent condensation.
• Aliquoting: To prevent freeze–thaw cycling, prepare single-use aliquots in low-protein-binding tubes. Minimize headspace and maintain aseptic technique.
• Stabilizers: For storage at −20°C, 30–50% glycerol is commonly added to prevent complete freezing and reduce aggregation; carrier protein (0.1–1% BSA) can enhance stability at low concentrations. Confirm compatibility with intended assays (e.g., avoid BSA for certain biotin/streptavidin or MS workflows).
• Reconstitution (if lyophilized; item state not specified): Reconstitute gently in sterile, neutral pH buffer (e.g., PBS) to the desired concentration. Avoid vigorous vortexing; mix by gentle inversion and allow to hydrate on ice for 10–15 minutes.
• Short-term handling: Keep on ice during use. Avoid repeated probe entries into stock vials; use fresh tips for each withdrawal.
• Long-term storage: For multi-month storage, maintain at −20°C (with glycerol) or below; for extended terms, −80°C may further enhance stability. Do not store at 4°C for prolonged periods unless formulation specifies preservatives.

Item-specific concentration, buffer composition, and presence of preservatives: Not specified for this item; refer to CoA/Spec Sheet.

Structure and Identity

High-quality research-grade monoclonal antibody recognizing ITM2B (Integral Membrane Protein 2B; also known as BRI2). This product is a mouse-derived monoclonal antibody (mAb).

• SKU: Ab111398
• Product Name: ITM2B Mouse mAb
• Category: Antibody (Monoclonal), Research use only

Item-specific identifiers:

• CAS: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey / SMILES: Not applicable to protein biologics (antibodies).
• Molecular Formula: Not applicable to heterogeneous glycoproteins; not specified for this item.

Structural features (general, literature):

• Immunoglobulin G (IgG)-type protein architecture with two heavy and two light chains connected by disulfide bonds; Y-shaped quaternary structure.
• Typical molecular weight for an intact IgG ~150 kDa (literature); exact MW varies by isotype and glycosylation.
• Post-translational features may include conserved N-linked glycosylation on Fc (literature). Stereochemical description is not applicable to macromolecular antibodies.

2D/primary structure description (general):

• Variable domains (VH/VL) constitute the antigen-binding fragments (Fabs) specific for ITM2B epitopes.
• Constant domains (CH1–CH3 and CL) and hinge region form the Fc and structural framework, enabling effector functions in immunochemical assays (not a clinical claim).

Synthetic Utility

Not typically applicable. As a monoclonal antibody, this product is a biological affinity reagent rather than a synthetic building block or reagent used in chemical transformations.

For chemists integrating bio- and chemistries (general guidance):

• Antibody–conjugate preparation: If creating labeled probes (e.g., HRP, fluorophores, biotin), standard bioconjugation chemistries (NHS-ester to lysines, maleimide to reduced cysteines, click chemistry to azide/alkyne handles) can be employed. Ensure that labeling stoichiometry preserves binding affinity and consider site-selective methods (e.g., Fc glycan oxidation) to minimize paratope modification.
• Solid support coupling: Immobilize antibodies on resins (CNBr-activated Sepharose, NHS-activated agarose) for immunoaffinity purification; optimize coupling pH and quenching to retain activity.

Item-specific conjugation readiness (buffer composition, amine content, preservatives) is not specified for this item; certain buffers (e.g., Tris, primary amines) and additives (e.g., azide) may interfere with labeling chemistries. Consult the CoA/Spec Sheet and exchange buffer if needed.

Target Specificity

Item-specific details from Product Data:

• Target antigen: ITM2B (Integral Membrane Protein 2B; also referred to as BRI2)
• Antibody type: Monoclonal antibody (mAb)
• Host: Mouse

Not specified for this item; refer to CoA/Spec Sheet:

• Clone ID: Not specified.
• Isotype (e.g., IgG1, IgG2a): Not specified.
• Species reactivity (human/mouse/rat, etc.): Not specified.
• Tested/validated applications (WB, IHC, IF, ELISA, IP): Not specified.
• Epitope mapping/peptide immunogen: Not specified.
• Cross-reactivity: Not specified.

Notes:

• For selection of secondary antibodies, plan for an anti-mouse secondary with appropriate isotype compatibility once the isotype is confirmed.
• Validate specificity in your system using genetic controls (KO/KD) or orthogonal methods whenever possible.

Perguntas frequentes

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (ITM2B Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.