JH-RE-06 - 10mM in DMSO , CAS No.1361227-90-8

CAS: 1361227-90-8 Cat. No.: J421371 Fórmula: C₂₀H₁₆Cl₃N₃O₄ Peso molecular: 468.72

GRADE & PURITY 10mM in DMSO

Synonyms

4(1H)​-​Quinolinone,8-​chloro-​2-​[(2,​4-​dichlorophenyl)​amino]​-​3-​(3-​methyl-​1-​oxobutyl)​-​5-​nitro-

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Visão geral

Information

JH-RE-06 JH-RE-06 is a potent REV1-REV7 interface inhibitor with an IC50 of 0.78 μM and Kd value of 0.42 μM, disrupting REV1-POL ζ-mediated mutagenic translesion synthesis (TLS).

Targets

REV1-REV7 (Cell-free assay); REV1-REV7 (Cell-free assay) 0.42 μM(Kd); 0.78 μM

In vitro

JH-RE-06 disrupts mutagenic TLS by preventing recruitment of mutagenic POL ζ. Remarkably, JH-RE-06 targets a nearly featureless surface of REV1 that interacts with the REV7 subunit of POL ζ. Binding of JH-RE-06 induces REV1 dimerization, which blocks the REV1-REV7 interaction and POL ζ recruitment. JH-RE-06 inhibits mutagenic TLS and enhances cisplatin-induced-toxicity in cultured human and mouse cell lines.

In vivo

JH-RE-06 improves tumor cell response to cisplatin in vivo. Co-administration of JH-RE-06 with cisplatin suppresses the growth of xenograft human melanomas in mice, establishing a framework for developing TLS inhibitors as a novel class of chemotherapy adjuvants.

Cell Research(from reference)

Cell lines：HT1080 cells, A375 cells, KP cells, MEF, LNCap cells, AG01522 cells. 

Concentrations：1.5 μM 

Incubation Time：24 h 

Specifications

Sinónimos

4(1H)​-​Quinolinone,8-​chloro-​2-​[(2,​4-​dichlorophenyl)​amino]​-​3-​(3-​methyl-​1-​oxobutyl)​-​5-​nitro-

Especificações e pureza

10mM in DMSO

Mecanismos bioquímicos e fisiológicos

JH-RE-06 is a potent REV1-REV7 interface inhibitor with an IC50 of 0.78 μM and Kd value of 0.42 μM, disrupting REV1-POL ζ-mediated mutagenic translesion synthesis (TLS).

Condições de armazenamento de armazenamento

Store at -80°C

Enviado em

Dry ice packs + Cold packs

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Tipo de ação

INHIBITOR

Propriedades do produto

• ALogP: 6.232
• hba_count: 2
• Contagem HBD: 2
• Ligação rotativa: 6

Nomes e identificadores

• Peso molecular: 468.72
• Reaxy-Rn: 22334922
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=22334922&ln=

Propriedades químicas e físicas

• DMSO (mg/mL) Solubilidade máxima: 12
• DMSO (mM) Solubilidade máxima: 25.6016385048643
• Água (mg/mL) Solubilidade máxima: ＜1

Perguntas frequentes

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1361227-90-8, the molecular formula is C20H16Cl3N3O4, and the molecular weight is 468.72 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.