Laboratory Protocols & SOPs Library

EC Medium (Powder)

Directions per Liter:

Dissolve 37grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi)


Component g/liter
Casein Digest Peptone 15
Deoxyribonucleic Acid 2
Papaic Digest of Soybeans 5
Sodium Chloride 5
Agar 15
Toluidine Blue O 0.1
Total: 37g/Liter


Storage and Stability:

Store powdered media at 4°C Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should stored at 4°C and used within a short period of time.

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "EC Medium (Powder)" Aladdin Knowledge Base, updated 09/06/2025. https://www.aladdinsci.com/eu_pt/faqs/ec-medium-powder-en.html

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