Recombinant Human GON4L Protein: Properties & Research Uses

Recombinant Human GON4L Protein is a research-grade recombinant protein targeting the GON4-like (GON4L) gene product, a highly conserved nuclear factor implicated in chromatin remodeling, transcriptional regulation, and cell fate determination.

Product Name

Recombinant Human GON4L Protein

SKU

rp370888

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant nuclear regulatory protein

Primary Research Areas

Chromatin biology, epigenetics, transcription regulation

Supplier

Aladdin Scientific

What Is GON4L and Why Does It Matter?

GON4L (GON4-like protein) is a large nuclear protein encoded by the GON4L gene in humans. It is the mammalian orthologue of the Caenorhabditis elegans GON-4 protein, which is essential for germline development. In vertebrates, GON4L has been associated with transcriptional regulation, chromatin organization, and hematopoietic cell development. The protein contains conserved domains including a paired amphipathic helix (PAH) domain and a plant homeodomain (PHD) zinc-finger-like motif, which are characteristic of transcriptional co-regulators that interact with chromatin-modifying complexes.

Research in zebrafish and mouse models indicates that GON4L plays a critical role in regulating gene expression programs during embryonic development and lineage specification, particularly in hematopoiesis. Its loss-of-function phenotypes underscore its non-redundant function in coordinating transcriptional networks that govern cell identity.

Product Description and Specifications

Aladdin Scientific's Recombinant Human GON4L Protein (SKU: rp370888) is produced to research-grade quality standards, making it suitable for use as a tool in biochemical and cell biology experiments. Recombinant expression systems allow researchers to obtain sufficient quantities of this large nuclear protein for in vitro studies, protein–protein interaction assays, and antibody validation purposes.

Researchers are advised to consult the product datasheet (referenced as absea#PP-5102) for complete specification details, including the expression host, tag information, molecular weight, purity grade, formulation buffer, and recommended storage conditions. All critical technical parameters should be confirmed directly from the official product documentation prior to experimental use.

• Product SKU: rp370888
• Reference Document: absea#PP-5102
• Protein Source: Human (Homo sapiens)
• Application: Research use only (RUO)

Research Applications

Recombinant Human GON4L Protein is a versatile tool for researchers investigating transcriptional control and chromatin biology. Key research applications include:

• Protein–Protein Interaction Studies: GON4L's conserved domains make it a candidate binding partner for chromatin remodeling complexes and transcription factors. Pull-down and co-immunoprecipitation (co-IP) assays using recombinant GON4L can help map interaction networks.
• Biochemical Activity Assays: Recombinant protein preparations enable in vitro characterization of GON4L's molecular activities, including its potential role in histone modification or transcriptional co-regulation.
• Antibody Validation: Recombinant GON4L serves as a positive control antigen for validating anti-GON4L antibodies used in western blotting, immunofluorescence, or ELISA applications.
• Structural Biology: Purified recombinant protein is a prerequisite for crystallography, cryo-EM, or NMR studies aimed at resolving GON4L domain structures.
• Hematopoiesis and Developmental Biology Research: Given GON4L's established role in blood cell development, recombinant protein can support mechanistic studies in hematopoietic stem and progenitor cell (HSPC) biology.

Handling, Storage, and Quality Considerations

Proper handling of recombinant proteins is essential to maintain activity and integrity. General best practices for recombinant nuclear proteins such as GON4L include:

• Storage: Store according to the conditions specified on the product datasheet. Recombinant proteins are typically stored at −20°C or −80°C in aliquots to avoid repeated freeze–thaw cycles.
• Reconstitution: If supplied lyophilized, reconstitute with the recommended volume of sterile, ultrapure water or the buffer specified in the datasheet. Allow to equilibrate gently; avoid vigorous vortexing.
• Aliquoting: Prepare single-use aliquots upon first thaw to preserve protein activity for downstream experiments.
• Quality Assurance: Aladdin Scientific subjects recombinant proteins to quality control testing. Consult absea#PP-5102 for lot-specific purity and activity data.

This product is intended for research use only and is not suitable for diagnostic or therapeutic applications in humans or animals.

Frequently asked questions

What is the GON4L gene and what does its protein product do?

GON4L encodes a large, conserved nuclear protein that functions as a transcriptional regulator involved in chromatin organization and cell fate specification. It is the vertebrate orthologue of C. elegans GON-4 and has been shown to be important in hematopoietic development and gene expression control during embryogenesis.

What applications is Recombinant Human GON4L Protein (rp370888) suitable for?

Recombinant Human GON4L Protein is suitable for research applications including protein–protein interaction studies (pull-down, co-IP), antibody validation (western blot, ELISA, immunofluorescence), in vitro biochemical assays, and structural biology investigations. It is for research use only and not intended for clinical or diagnostic purposes.

How should Recombinant Human GON4L Protein be stored to maintain stability?

Recombinant proteins should generally be stored at −20°C or −80°C in single-use aliquots to prevent activity loss from repeated freeze–thaw cycles. Specific storage conditions, buffer composition, and shelf life for rp370888 are detailed in the product datasheet (absea#PP-5102). Always refer to the datasheet for lot-specific guidance.

Where can I find the full technical specifications for rp370888?

The complete technical specifications for Recombinant Human GON4L Protein (rp370888), including expression host, tag, purity, molecular weight, formulation, and recommended applications, are available in the product datasheet referenced as absea#PP-5102. Contact Aladdin Scientific technical support for additional information.

References

1. Product Datasheet: Recombinant Human GON4L Protein, absea#PP-5102. Aladdin Scientific.
2. GON4L gene entry. NCBI Gene Database. National Center for Biotechnology Information.
3. Textbook-level knowledge: GON4L as a conserved nuclear transcriptional regulator and vertebrate orthologue of C. elegans GON-4; role in hematopoiesis — general scientific literature.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.