Laboratory Protocols & SOPs Library

Tryptone Broth (Powder)

Directions per Liter:

Dissolve 15 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).


Component g/liter
Sodium Chloride 5
Tryptone 10
Total: 15g/Liter
500g makes 33.3 liters


Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


BSE-Free: All raw materials of animal origin are sourced from USDA-designated BSE-Free countries.

Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates.

Homogeneous: Proprietary blending technology ensures complete mixing of components.

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.

Cite this article

Aladdin Scientific. "Tryptone Broth (Powder)" Aladdin Knowledge Base, updated 09/06/2025. https://www.aladdinsci.com/eu_pt/faqs/tryptone-broth-powder-en.html

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