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Does the protein's own charge affect the buffer?
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Does the protein's own charge affect the buffer?
No. The SDS in the buffer binds to the protein and completely covers the protein-SDS complex. The SDS in the buffer binds to the protein and causes the protein-SDS complex to take on a large negative charge, completely covering the protein's own charge.
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Aladdin Scientific. "Does the protein's own charge affect the buffer?" Aladdin Knowledge Base, updated 10/11/2026. https://www.aladdinsci.com/eu_pt/faqs/does-the-protein-own-charge-affect-the-buffer-en.html
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