≥95% for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Protected from light,Store at -20°C,Argon charged Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
L67 (DNA Ligase Inhibitor) is a competitive DNA ligase inhibitor that effectively inhibits DNA ligases I/III (both IC 50 are 10 μM). L67 (DNA Ligase Inhibitor) can cause nuclear DNA damage by reducing levels of mitochondrial DNA and increasing levels of mitochondrially-generated ROS. L67 (DNA Ligase Inhibitor) also activates the Caspase 1 -dependent apoptosis pathway in cancer cells, can be used in cancer research.
In Vitro
L67 (10, 15 μM; 24 h) promotes nuclear DNA damage and (0-50 μM) increases level of mSOX by inhibiting mitochondrial LigIIIα in HeLa cells. L67 (10 μM; 24 h) induces changes in mitochondrial function that causes a reduction in OCR and mitochondrial DNA, and abnormal mitochondrial morphology in HeLa. L67 (10, 100 μM; 24 h) induces apoptosis in cancer cells. L67 (0-30 μM; 24 h) selectively induces cell death in cancer cells by activating a caspase 1-dependent apoptotic pathway. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: HeLa cells Concentration: 10, 15 μM; 0-50 μM Incubation Time: 24 h Result: Increased the formation of nuclear γH2AX foci and steady state levels of γH2AX when at 10 or 15 μM. (γH2AX: a sign of DNA double-strand breaks). Resulted in a concentration (0-50 μM)-dependent increase in mSOX (mitochondrial superoxide) levels. (mSOX is a major cause of the cellular oxidative damage). Cell Viability AssayCell Line: HeLa cells Concentration: 10 μM Incubation Time: 24 h Result: Reduced oxygen consumption rate (OCR) approximately 20%. Resulted in about a 25% reduction in mitochondrial DNA. Apoptosis AnalysisCell Line: HeLa cells Concentration: 10, 100 μM Incubation Time: 24 h Result: Result:Induced apoptosis, and at 100 μM with apoptotic cells constituting about 50% of the HeLa cell population. Cell Viability AssayCell Line: HeLa cells Concentration: 0-30 μM Incubation Time: 24 h Result: Activates a caspase 1-dependent cell death pathway in cancer cells.
Form:Solid
| Pubchem Sid | 528765263 |
|---|---|
| Sorrisos canónicos | CC1=C(C=C(C=C1Br)NCC(=O)NN=CC2=C(C=CC(=C2)[N+](=O)[O-])O)Br |
| IUPAC Name | 2-(3,5-dibromo-4-methylanilino)-N-[(E)-(2-hydroxy-5-nitrophenyl)methylideneamino]acetamide |
| InChIKey | KFEPVFJQVOMODD-IFRROFPPSA-N |
| INCHI | 1S/C16H14Br2N4O4/c1-9-13(17)5-11(6-14(9)18)19-8-16(24)21-20-7-10-4-12(22(25)26)2-3-15(10)23/h2-7,19,23H,8H2,1H3,(H,21,24)/b20-7+ |
| SMILES isoméricas | CC1=C(C=C(C=C1Br)NCC(=O)N/N=C/C2=C(C=CC(=C2)[N+](=O)[O-])O)Br |
| PubChem CID | 135419707 |
| Peso molecular | 486.11 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Taxonomy Tree
| Kingdom | Organic compounds |
|---|---|
| Superclass | Benzenoids |
| Classe | Phenols |
| Subclass | Nitrophenols |
| Intermediate Tree Nodes | Not available |
| Direct Parent | Nitrophenols |
| Alternative Parents | Nitrobenzenes Phenylalkylamines Nitroaromatic compounds Aniline and substituted anilines Aminotoluenes Secondary alkylarylamines Bromobenzenes 1-hydroxy-2-unsubstituted benzenoids Azines Aryl bromides Propargyl-type 1,3-dipolar organic compounds Organic oxoazanium compounds Organopnictogen compounds Organooxygen compounds Organobromides Organic zwitterions Organic oxides Hydrocarbon derivatives |
| Molecular Framework | Aromatic homomonocyclic compounds |
| Substituents | Nitrophenol - Nitrobenzene - Nitroaromatic compound - Aminotoluene - Phenylalkylamine - Aniline or substituted anilines - 1-hydroxy-2-unsubstituted benzenoid - Toluene - Secondary aliphatic/aromatic amine - Halobenzene - Bromobenzene - Monocyclic benzene moiety - Azine - Aryl halide - Aryl bromide - Organic nitro compound - C-nitro compound - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Allyl-type 1,3-dipolar organic compound - Secondary amine - Organic oxoazanium - Organic nitrogen compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Organic zwitterion - Organooxygen compound - Organonitrogen compound - Organobromide - Organohalogen compound - Amine - Aromatic homomonocyclic compound |
| Descrição | This compound belongs to the class of organic compounds known as nitrophenols. These are compounds containing a nitrophenol moiety, which consists of a benzene ring bearing both a hydroxyl group and a nitro group on two different ring carbon atoms. |
| External Descriptors | Not available |
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
|---|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Data | Item |
|---|---|---|---|
 J2528534 | Certificate of Analysis | Aug 27, 2025 | L651811 |
| J2528535 | Certificate of Analysis | Aug 27, 2025 | L651811 |
| J2528536 | Certificate of Analysis | Aug 27, 2025 | L651811 |
| J2528537 | Certificate of Analysis | Aug 27, 2025 | L651811 |
| Solubilidade | DMSO : ≥ 33 mg/mL (67.89 mM) H2O : 1 mg/mL (2.06 mM; Need ultrasonic) |
|---|---|
| Sensibilidade | Light sensitive; Air sensitive |
| Peso molecular | 486.100 g/mol |
| XLogP3 | 4.100 |
| Hydrogen Bond Donor Count | 3 |
| Hydrogen Bond Acceptor Count | 6 |
| Rotatable Bond Count | 5 |
| Exact Mass | 485.936 Da |
| Monoisotopic Mass | 483.938 Da |
| Topological Polar Surface Area | 120.000 Ų |
| Heavy Atom Count | 26 |
| Formal Charge | 0 |
| Complexity | 519.000 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 1 |
| Undefined Bond Stereocenter Count | 0 |
| The total count of all stereochemical bonds | 1 |
| Covalently-Bonded Unit Count | 1 |
