See COA para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.
🌡
Armazenamento e envio
Store at -20°C,Avoid repeated freezing and thawing Envio Ice chest + Ice pads. Consulte o COA específico do lote para obter as especificações exatas.
📋
Documentos de qualidade
SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.
📚
Comprovação documental
Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.
Visão geral
Mouse anti Human LAMP1/CD107a Antibody, Monoclonal (OTI8B1), could be used for WB, IHC, Flow Cytometry and so on.
Especificações
Product Name
LAMP1/CD107a Mouse mAb - Primary antibody, specific to LAMP1
Sinónimos
CD107 antigen like family member A antibody | CD107 antigen-like family member A antibody | CD107a antibody | CD107a antigen antibody | LAMP 1antibody | LAMP-1 antibody | LAMP1 antibody | LAMP1_HUMAN antibody | LAMPA antibody | LGP120antibody | lgpA antib
Especificações e pureza
See COA
Mecanismos bioquímicos e fisiológicos
Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.
Post-translational: O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Revisões
Avaliações dos Clientes
Application Protocols
Tested/validated applications, recommended dilutions, positive controls, and stepwise protocols are not specified for this item; refer to the CoA/Spec Sheet for SKU Ab112729.
General best-practice notes (non-item-specific, no implied validation):
Include appropriate positive and negative biological controls (e.g., cells/tissues known to express or lack LAMP1/CD107a).
Titrate the antibody in your matrix to determine the optimal working concentration.
For intracellular lysosomal staining, fix and permeabilize appropriately; for degranulation readouts (CD107a surface exposure), perform staining on live cells during/after stimulation.
Match secondary antibodies to the primary’s host species and isotype (isotype for this item not specified; verify before use).
Please rely on lot-specific documentation for any guaranteed applications or performance claims.
Biological Roles
Target: LAMP1 (CD107a) — literature overview
Molecular identity and localization:
LAMP1 is a heavily glycosylated type I transmembrane glycoprotein predominantly localized to lysosomal and late endosomal membranes. Its luminal domain is rich in N-linked glycans that protect the lysosomal membrane from the lumen’s hydrolytic environment.
Cellular functions (literature):
Contributes to lysosomal membrane integrity, trafficking, and fusion events, including autophagosome–lysosome and endosome–lysosome fusion.
Participates in lysosomal exocytosis and degranulation processes. Transient surface expression of LAMP1 (CD107a) serves as a robust marker of degranulation in cytotoxic T lymphocytes (CTLs), NK cells, and certain myeloid cells.
Involved in receptor-mediated signaling and interactions with cytosolic adaptors via its short cytoplasmic tail containing sorting motifs (e.g., tyrosine-based signals) that mediate clathrin/AP complex interactions.
Research utility (literature):
Widely used as a marker of lysosomes in fixed-cell imaging and as a readout of cytotoxic granule release in flow cytometry-based CD107a assays.
Employed to study lysosome biogenesis, autophagy/mitophagy flux, endolysosomal dynamics, and immune cell activation states.
Orthologs and expression:
Conserved across mammalian species with cell type–dependent expression; high in cells with active endolysosomal pathways.
Notes: The above reflects general biology of LAMP1/CD107a. Specific epitope recognition, species cross-reactivity, and assay validation for this item are not specified; consult the CoA/Spec Sheet for item-level details.
Buffer Applications
While buffer recipes for this specific item are not provided, the following general guidance applies to monoclonal antibodies used in immunoassays.
Common working buffers (general):
PBS (phosphate-buffered saline) or TBS (Tris-buffered saline) for dilution and washing; add Tween-20 (0.05–0.1%) for immunoblots and some IHC/IF washes to reduce nonspecific binding.
Blocking solutions: BSA, non-fat dry milk, fish gelatin, or serum matched to the host of the secondary antibody. Choice depends on assay (WB vs IHC/IF vs FC) and potential cross-reactivity.
For flow cytometry (CD107a degranulation assays), protein-containing buffers (e.g., PBS + 1–2% FBS or BSA) help minimize cell stress and nonspecific binding (general practice; item-specific validation not provided).
Additives and compatibility:
If the antibody contains preservatives (e.g., azide) or carrier proteins, verify compatibility with live-cell assays and conjugation chemistries; this item’s formulation is not specified.
For immobilization or conjugation, use amine-free buffers when employing NHS-ester reagents.
pH and ionic strength:
Maintain near-physiologic pH (~7–8) and isotonic ionic strength to preserve antibody conformation and target binding (general literature guidance).
Consult the item-specific CoA/Spec Sheet for any mandated buffer conditions or restrictions.
Green Alternatives
Green chemistry comparisons are not directly applicable to an antibody reagent, as it is used in aqueous buffered systems and not as a conventional organic solvent or reagent in synthetic chemistry.
Sustainability considerations (general):
Minimize single-use plastics by employing reusable secondary containers and low-bind consumables judiciously.
Reduce cold-chain energy by consolidating shipments and aliquoting to decrease freeze–thaw-related waste.
Where possible, select preservative systems and buffer components with lower environmental persistence; the formulation of this item is not specified.
Process suggestions:
Use efficient blocking/washing protocols to reduce buffer volumes.
Employ room-temperature incubation steps when validated to save energy (maintaining sample integrity and antibody performance).
Given the biological nature of this product, “green alternatives” are better framed as best practices for resource and waste reduction in immunoassay workflows rather than substitution with other chemicals.
Pharmaceutical Uses
This product is a research-use-only monoclonal antibody and is not intended for human or veterinary therapeutic or diagnostic applications.
In pharmaceutical R&D labs, anti-LAMP1 antibodies can be used as analytical reagents in characterization assays (e.g., cell-based potency readouts, flow cytometry markers for degranulation) to support discovery-stage studies. Such use is strictly non-clinical.
Antibodies employed in manufacturing analytics are often required to be low in endotoxin and free from interfering stabilizers; specifications for this item are not provided here and must be confirmed via CoA/Spec Sheet if critical to your workflow.
Regulatory note:
Not GMP-grade. No pharmacopeial monograph applies to an RUO research antibody of this type. Do not use in lot-release or clinical testing without appropriate qualification/validation and sourcing of compliant materials.
Practical considerations:
For conjugation to reporters (e.g., enzyme, fluor), ensure formulation compatibility and perform post-conjugation clean-up to remove unreacted label—general best practice; item formulation is not specified.
Any implication of clinical or diagnostic performance is outside the scope of this RUO product.
Physical Properties
Item-specific physicochemical parameters (exact values) are not provided for this antibody preparation.
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Concentration and formulation (buffer, stabilizers, preservatives): Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet. (General literature: intact IgG ~150 kDa; may present ~50 kDa heavy and ~25 kDa light chains under reducing SDS-PAGE.)
pI and extinction coefficient: Not specified for this item; refer to CoA/Spec Sheet. (General literature: IgG pI commonly ~6–9; A280 ε depends on sequence and glycosylation.)
Solubility: Antibodies are typically soluble in isotonic aqueous buffers (e.g., PBS, TBS) over a wide pH range (literature). Specific buffer compatibility for this item is not specified.
Sensitive to repeated freeze–thaw cycles, which can induce aggregation and loss of activity.
Temperature-dependent denaturation; maintain cold chain and avoid elevated temperatures.
Surface adsorption to untreated plastics at low concentrations; low-bind plastics or carrier proteins can mitigate (if compatible with downstream use).
No boiling point, melting point, vapor pressure, logP, refractive index, or similar small-molecule properties are applicable to antibodies. For exact storage buffer composition, preservative content, and concentration, consult the CoA/Spec Sheet for this specific lot.
Quality and Grades
Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
Interpreting quality for monoclonal antibodies (general, for professional users):
Purity is commonly assessed by SDS-PAGE (reducing/non-reducing) and size-exclusion chromatography; typical high-quality mAbs show a dominant monomer peak with minimal aggregates/Fragments.
Identity/Specificity may be supported by ELISA against antigen, immunocytochemistry controls, flow cytometry on known-positive/negative cells, or knockout/knockdown validation.
Concentration is often determined by A280 or BCA; extinction coefficient is sequence-dependent (item-specific value not provided).
Endotoxin level (EU/mg), bioburden, and residual host cell proteins/DNA (for recombinant formats) may be reported for applications sensitive to innate immune activation; values for this item are not specified.
Isotype/subclass and light chain type (e.g., IgG1, kappa) guide selection of secondary antibodies and protein A/G purification; not specified here.
Stabilizers/preservatives:
Formulation components (e.g., glycerol, BSA, trehalose, sodium azide) influence storage, compatibility with functional assays, and downstream conjugations. This item’s formulation is not specified; verify before use in live-cell assays or conjugation workflows.
Lot-to-lot documentation:
For certificates of analysis (CoA), validation data, and specifications (purity, concentration, buffer), consult the lot-specific documentation for SKU Ab112729.
Reaction and Applications
This section (chemical reactions) is generally not applicable to antibodies, which are biological affinity reagents rather than small-molecule reactants or catalysts.
Relevant applications for this product class are immunoassays and cell biology workflows (e.g., immunofluorescence, flow cytometry, Western blotting, immunohistochemistry, immunoprecipitation). However, tested/validated applications and conditions for this specific item are not specified; consult the CoA/Spec Sheet.
Conjugation (general guidance):
Antibodies can be derivatized with fluorophores, enzymes, or biotin using NHS ester, maleimide, or click chemistries after buffer exchange into compatible media; verify that the formulation of this item is amenable before attempting conjugation.
For protocol-specific details (incubation times, dilutions, controls), please refer to the lot-specific documentation or perform in-house optimization.
Reaction Conditions
Chemical reaction conditions are not applicable to this antibody product. For immunoassay workflows, conditions are assay-specific and must be optimized empirically.
General immunoassay considerations (non-item-specific):
Temperature: Many antibody incubations proceed at room temperature or 4°C depending on assay sensitivity and background; exact conditions for this item are not specified.
Time: Incubation times vary (minutes to overnight) based on target abundance and matrix; optimize per application.
Matrix: Fixed cells/tissues (IF/IHC), lysates (WB/IP), or live cells (FC). Permeabilization required for intracellular LAMP1 staining in fixed specimens; surface CD107a detection during degranulation assays typically on live cells (general literature guidance).
Refer to the CoA/Spec Sheet for any validated conditions or recommended starting points specific to SKU Ab112729.
Safety and Handling
GHS/SDS information:
Signal Word: Not specified for this item; refer to SDS.
Hazard Statements (H-phrases): Not specified for this item; refer to SDS.
Pictograms/GHS Classification: Not specified for this item; refer to SDS.
General safety guidance for protein antibodies (laboratory use):
For research use only; not for human or animal therapeutic/diagnostic use.
Handle using good laboratory practice. Wear appropriate PPE: lab coat, safety glasses, and disposable nitrile gloves.
Avoid ingestion, inhalation of aerosols, and contact with eyes/skin. Work in a clean area to avoid microbial contamination.
If material contains preservatives (e.g., sodium azide) or carrier proteins, follow SDS for specific hazards; this item’s formulation is not specified.
First aid (general):
Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing. Seek medical attention if irritation persists.
Ingestion/inhalation: Rinse mouth, move to fresh air; seek medical advice as needed.
Handling precautions:
Avoid repeated freeze–thaw cycles; aliquot if appropriate.
Keep cold during use; store promptly per label after handling.
Use low-protein-binding tips/tubes to minimize loss at low concentrations.
Incompatibilities and conditions to avoid:
Elevated temperatures, extreme pH, and mechanical foaming/shear that can denature proteins.
Microbial contamination; practice aseptic technique when necessary.
Always consult the item-specific SDS for authoritative safety and hazard information.
Solvent Selection
Solvent selection is not typically applicable to monoclonal antibodies, which are supplied and used in aqueous buffers rather than organic solvents.
Practical notes (general):
Use isotonic aqueous buffers (e.g., PBS, TBS) compatible with your assay. Avoid organic solvent exposure, high ionic strength shifts, or drastic pH changes unless validated for your protocol.
For conjugation chemistries, low-amine or amine-free buffers (e.g., PBS without Tris/glycine) may be preferred when using NHS esters; this item’s buffer is not specified—exchange/desalt if needed.
For cell-based work, ensure buffers are free of cytotoxic preservatives; formulation for this item is not specified.
If you require a specific buffer system (detergent-free, azide-free, carrier-free), consider buffer exchange or dialysis using appropriate low-binding devices after confirming the current formulation on the CoA/Spec Sheet.
Storage and Reconstitution
Storage conditions (item-specific):
Store at -20°C.
Avoid repeated freezing and thawing.
Shipped in an ice chest with ice pads to maintain cold chain.
Aliquoting and handling (general best practices):
Upon first receipt/opening, keep cold and, if freezing is required, prepare single-use aliquots in low-protein-binding tubes to minimize freeze–thaw cycles.
Thaw on ice and mix gently; avoid vortexing/foaming that may denature proteins.
Do not store in frost-free freezers that cycle temperatures.
Reconstitution and formulation: Not specified for this item; refer to CoA/Spec Sheet.
If supplied lyophilized (not specified), reconstitute with sterile, antibody-compatible buffer per the CoA and allow complete dissolution on ice.
If supplied in liquid form (not specified), maintain the recommended storage temperature and avoid contamination.
Long-term stability: Not specified for this item; refer to CoA/Spec Sheet. For extended storage, some users maintain aliquots at ≤ -70°C (general practice) if compatible with the formulation.
Always consult the lot-specific CoA/Spec Sheet for exact buffer composition, presence of preservatives/stabilizers, and any additional storage instructions unique to this product.
Structure and Identity
Professional-grade mouse monoclonal antibody directed against lysosomal-associated membrane protein 1 (LAMP1/CD107a).
Item-specific identifiers:
Product Name: LAMP1/CD107a Mouse mAb (SKU: Ab112729)
CAS: Not applicable to antibodies; not registered
Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
SMILES / InChI / InChIKey: Not applicable for complex biologics; not provided
Structural features (general, literature-based for mouse IgG mAbs):
Typical IgG architecture with two identical heavy chains and two identical light chains forming a Y-shaped heterotetramer stabilized by inter- and intrachain disulfide bonds.
Variable regions (VH/VL) define antigen recognition; constant regions (CH/CL) determine isotype and Fc effector functions (isotype for this item not specified).
Glycoprotein: conserved N-linked glycan(s) on the Fc region (composition may vary; item-specific profile not provided).
2D/primary structure description (general):
Modular immunoglobulin domains (beta-sandwich folds) arranged as Fab arms (antigen-binding) and an Fc stem (effector). The antigen-binding site is formed by six CDR loops.
Target identity (from product name):
Antigen: LAMP1 (CD107a), a type I lysosomal membrane glycoprotein.
Host: Mouse; Monoclonal antibody.
Notes: Any clone designation, epitope mapping, isotype/subclass, and sequence are not specified for this item and should be confirmed from the CoA/Spec Sheet.
Synthetic Utility
Not applicable. This product is a biological affinity reagent (mouse monoclonal antibody) and is not used as a synthetic building block, catalyst, or stoichiometric reagent in chemical synthesis.
Related practical note (general):
If chemical modification is desired (e.g., fluorophore, biotin, or drug-linker attachment), standard bioconjugation chemistries (NHS ester to lysines, maleimide to reduced cysteines, enzymatic glycan remodeling, or click chemistry) may be applied after buffer exchange into compatible media. The present item’s buffer composition and suitability for conjugation are not specified; verify via CoA/Spec Sheet.
Target Specificity
Item-specific details are limited to the product name.
Clone, epitope, species reactivity, isotype, and cross-reactivity: Not specified for this item; refer to CoA/Spec Sheet.
For assurance of suitability in your system (species/cell line), please consult lot-specific validation data.
Perguntas frequentes
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (LAMP1/CD107a Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Usamos cookies para garantir o funcionamento adequado do site e, quando permitido, para melhorar a sua experiência. Você pode gerenciar suas preferências a qualquer momento nas Configurações. Saiba mais em nossa Política de Cookies.
Deseja que lhe enviemos uma mensagem quando tivermos descontos disponíveis?
Lembrar-me mais tarde
Obrigado! Por favor, verifique a sua caixa de entrada de e-mail para confirmar.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
