MacConkey Broth (EP) - BioReagent

Cat. No.: M1528518

GRAU & PUREZA BioReagent

Storage

Room temperature

Shipped In

Normal

Visão geral

For coliform determination. Preparation method: Weigh 35.0 g of this product in 1 L of distilled or deionized water (the volume may be scaled up or down proportionally), heat to boiling until completely dissolved, autoclave at 121°C for 15 minutes, and set aside for use.

Especificações

Especificações e pureza

BioReagent

Condições de armazenamento de armazenamento

Room temperature

Enviado em

Normal

Grau

BioReagent

Product Line

Produtos biológicos

Application Protocols

The following is a general, literature-based workflow for research and non-clinical QC use. It is not item-specific; verify against your SOPs and the product CoA/label.

• Preparation
  • Weigh the recommended mass of dehydrated medium (e.g., 30–40 g/L; literature) into purified water with stirring.
  • Heat to near-boiling to dissolve completely; avoid scorching. Distribute into culture vessels (with optional Durham tubes if gas detection is desired).
  • Sterilize by autoclaving at 121 °C for ~15 minutes. Cool to 45–50 °C if dispensing post-sterilization.
• Quality checks
  • Confirm pH at 25 °C is within the method’s acceptable range (literature: ~7.1 ± 0.2).
  • Perform growth promotion testing with appropriate control strains as required by EP/your SOP.
• Use
  • Inoculate with test sample or control organism using aseptic technique.
  • Incubate aerobically at 35–37 °C for 18–24 h (adjust per method).
  • Observe: Acid from lactose fermentation turns the indicator red/pink; turbidity indicates growth. Non-fermenters typically do not acidify the medium.
• Disposal
  • Autoclave spent cultures and media prior to disposal. Follow institutional and local regulations for dye-containing wastes.

Note: For solid-phase applications, prepare MacConkey Agar per its specific formulation; this broth is intended for liquid culture.

Biological Roles

MacConkey Broth (EP) itself has no intrinsic biological role; rather, its components are formulated to provide selective growth and differential responses in microbiological experiments (literature, general):

• Peptones/tryptones: Complex nitrogen sources, peptides, amino acids, vitamins—support growth of many heterotrophic bacteria.
• Lactose (typical carbohydrate): Fermentable substrate for organisms possessing lactose transport and catabolic pathways (e.g., β-galactosidase), producing acidic metabolites that lower pH.
• Bile salts: Mimic intestinal bile, selecting for organisms tolerant to bile and inhibiting many Gram-positive species.
• Sodium chloride: Maintains osmotic balance.
• Neutral red: pH indicator (colorless to yellow above ~pH 6.8; red/pink in acidic range), enabling detection of acidification from lactose fermentation.
• (In some formulations) Crystal violet: Enhances inhibition of Gram-positive organisms.

Functionally, the medium differentiates lactose-fermenting Gram-negative bacteria (acid production detected by indicator) from non-fermenters under standard incubation. These properties support microbial physiology studies, strain characterization, and non-clinical quality control workflows.

Buffer Applications

Not typically applicable. MacConkey Broth (EP) is a nutrient medium with selective/differential properties, not a defined buffering system. While it exhibits some pH buffering capacity due to its complex components, it is not used as a standard laboratory buffer. For buffering needs, select appropriate defined buffers (e.g., phosphate, HEPES) as dictated by your application.

Green Alternatives

As a dehydrated microbiological medium, MacConkey Broth (EP) does not have conventional “green solvent” alternatives. Nonetheless, the environmental footprint of its use can be reduced through process choices.

• Resource-efficient practices:
  • Prepare only the volume needed to minimize waste and autoclave cycles.
  • Use energy-efficient autoclaves and consolidate sterilization loads where permissible.
  • Employ reusable, durable glassware for media preparation and culture where appropriate.
• Waste minimization:
  • Neutralize and autoclave spent media before disposal to prevent biological contamination; segregate dye-containing wastes per local regulations.
  • Avoid excessive indicator concentrations or unnecessary additives that complicate wastewater treatment.
• Alternatives (functional, not greener per se): If different selectivity is required, choose media tailored to the target organisms (e.g., eosin methylene blue, Hektoen enteric). Selection should be based on performance needs; environmental benefits are context-dependent.

Trade-offs:

• Ready-to-use sterile broths reduce in-lab energy use but may increase packaging and shipping footprint.
• In-house preparation allows right-sizing volumes but requires autoclave energy and water.

Adopt institution-specific green lab guidelines (e.g., My Green Lab) to optimize media preparation and sterilization workflows.

Pharmaceutical Uses

No therapeutic/clinical use. In pharmaceutical and related regulated laboratories, MacConkey media are commonly referenced in pharmacopeial microbiological tests as part of non-clinical quality control workflows (literature, general):

• European Pharmacopoeia context: Employed in growth promotion testing (GPT), media suitability, and selective enrichment/differentiation steps for specified test strains.
• Typical roles in QC labs: Examination of non-sterile products for specified microorganisms, water and environmental monitoring programs, and confirmation/differentiation steps in microbial limits testing—when prescribed by compendial methods and internal SOPs.
• Documentation: Use lots accompanied by a CoA; perform GPT with compendial strains as required. Record lot numbers, preparation parameters, and GPT outcomes.

All uses are for research and non-clinical quality control purposes only. This product is not an API, excipient, or therapeutic; it is a culture medium supporting microbiological testing workflows.

Physical Properties

As a dehydrated medium, MacConkey Broth (EP) presents as a blended powder. Item-specific physicochemical specifications (e.g., exact color, pH of prepared medium, moisture content) can vary by lot and are defined by the supplier’s CoA.

• Appearance (dehydrated): Not specified for this item; refer to CoA/Spec Sheet. (Typically a tan to pink-red, free-flowing powder; literature)
• Solubility: Freely soluble in water (literature, for intended preparation). Insoluble in most organic solvents.
• pH (prepared medium, literature): Typically around pH 7.1 ± 0.2 at 25 °C when prepared per manufacturer instructions. Not specified for this item; refer to CoA/Spec Sheet.
• Density, melting/boiling point, refractive index, logP: Not applicable to the dehydrated blend.
• Typical prepared medium appearance (literature): Clear to slightly opalescent, rose to red due to neutral red indicator; intensity depends on formulation and concentration.
• Ionic strength/osmolality (prepared): Not specified for this item; refer to CoA/Spec Sheet.

Notes:

• Because this is a mixture, single-compound constants (bp/mp/MW) are not meaningful.
• Preparation quality (complete dissolution, clarity) depends on water quality and proper heating without overheating the indicator.
• Always verify the target pH and preparation instructions on the product label/CoA for the specific lot.

Quality and Grades

• Grade: BioReagent (item-specific from Product Data). This indicates suitability for laboratory and research applications, emphasizing consistent performance in biological workflows such as microbial culture and quality control testing.
• EP designation: “(EP)” indicates the formulation is intended to comply with relevant European Pharmacopoeia specifications for MacConkey medium when prepared as directed. Compliance should be confirmed per lot via the CoA/specification sheet and, when applicable, by internal growth-promotion testing (GPT).
• What EP alignment implies (general): Defined performance characteristics, including selectivity and differential response (e.g., lactose fermentation response with indicator change), and pH/composition targets within pharmacopeial tolerances.
• Stabilizers/preservatives: Not typically applicable to dehydrated media; any such additives would be disclosed on the CoA. Not specified for this item; refer to CoA/Spec Sheet.
• UV/trace contaminants: Not applicable as a solvent/reagent metric; performance is validated by microbiological criteria (clarity, pH, GPT) rather than spectrophotometric cutoffs.

Recommendations:

• Perform GPT with relevant challenge organisms as required by EP/compendial methods and internal SOPs.
• Use high-purity water (e.g., purified/distilled) to meet EP performance claims when reconstituting.
• Document lot numbers and retain CoA copies for regulated workflows.

Reaction and Applications

This section focuses on laboratory applications, not chemical reactions. MacConkey Broth (EP) is a selective and differential liquid medium for the cultivation and differentiation of Gram-negative enteric bacteria based on lactose fermentation (literature, general microbiology).

• Selectivity: Bile salts (and, in some formulations, trace crystal violet) inhibit many Gram-positive organisms, enriching for Gram-negative enterics.
• Differential response: Lactose as a fermentable carbohydrate; neutral red detects acid production. Fermenters acidify the medium, shifting the indicator to red/pink; non-fermenters typically do not change the indicator color (literature).
• Typical workflows (research/industrial microbiology):
  • Enrichment and presumptive differentiation of lactose-fermenting vs. non-fermenting Gram-negative organisms.
  • Upstream or parallel use with solid MacConkey Agar for isolation and colony morphology assessment.
  • Quality control of raw materials, process waters, and environmental samples in research and non-clinical industrial settings.
• Practical tips:
  • Verify pH of prepared medium at room temperature; out-of-range pH can reduce selectivity or indicator response.
  • Use appropriate incubation conditions (e.g., 35–37 °C, 18–24 h; literature) and include positive/negative control strains for method verification.
  • Avoid overheating/over-autoclaving to prevent indicator degradation and sugar caramelization that may affect results.

Note: All uses are for research or non-clinical laboratory applications only; comply with relevant standards and SOPs.

Reaction Conditions

This section is repurposed to outline typical preparation and incubation conditions for microbiological use (general literature guidance). These are not item-specific specifications; consult the product label/CoA for definitive instructions.

• Reconstitution concentration (literature): Commonly prepared at approximately 30–40 g of dehydrated medium per liter of purified water, depending on formulation.
• Dissolution: Suspend in cold or room-temperature water with stirring; heat to near-boiling to ensure complete dissolution. Avoid prolonged boiling to prevent degradation of the pH indicator and sugars.
• Sterilization: Autoclave at 121 °C for ~15 minutes. Do not over-sterilize.
• Target pH (at 25 °C; literature): Approximately 7.1 ± 0.2 when prepared as directed. Verify and adjust only if allowed by method/SOP.
• Incubation (literature): Inoculate and incubate aerobically at 35–37 °C for 18–24 h (time/temperature may vary by method and organism).
• Interpretation (broth): Acid production from lactose fermentation shifts neutral red to red/pink; non-fermenters typically leave the medium unchanged. Gas production may be captured with a Durham tube where applicable.

Item-specific preparation details (exact grams per liter, pH targets, sterilization hold times): Not specified for this item; refer to CoA/Spec Sheet and your validated SOP.

Safety and Handling

GHS classification and pictograms for this specific product are not provided here. Handle according to good microbiological and chemical hygiene practices and consult the SDS for authoritative safety information.

• Signal word / H-statements / GHS class / pictograms: Not specified for this item; refer to SDS.
• Primary hazards (general for dehydrated media; literature): May cause eye/skin/respiratory irritation; colored indicators (e.g., neutral red, crystal violet in some formulations) can stain and may present additional hazards. Avoid inhalation of dust.
• PPE: Lab coat, gloves, and safety glasses at minimum. Use a dust mask/respirator if airborne dust is generated (per risk assessment/SDS).
• Engineering controls: Prepare solutions in a well-ventilated area or fume hood to minimize dust and vapors from boiling.
• Incompatibilities: Strong oxidizers may degrade organic components and dyes. Avoid contact with strong acids/bases that can irreversibly alter indicator function.
• Thermal processing: When sterilizing by autoclave, do not tightly cap vessels. Allow pressure to equilibrate; beware of superheating and sudden boiling on removal.
• Microbiological safety: Prepared broth will support microbial growth—treat all used media and cultures as biohazardous. Decontaminate by autoclaving before disposal.
• First aid (overview; defer to SDS): If inhaled—move to fresh air. Skin/eye contact—rinse with water for several minutes; remove contaminated clothing. If ingested—rinse mouth. Seek medical attention if irritation persists.

Always defer to the SDS and institutional EHS guidance for detailed handling and emergency measures.

Solvent Selection

This product is a dehydrated culture medium intended to be dissolved in water; conventional organic solvent selection is not applicable.

• Primary solvent: Purified water (e.g., EP/USP Purified Water or equivalent).
• Miscibility: Components are formulated to dissolve/disperse completely in water when heated to near-boiling as directed (literature). Incomplete dissolution may indicate inadequate heating or poor water quality.
• Water quality considerations:
  • Low ionic burden and minimal chlorine/chloramine help maintain indicator performance and clarity.
  • Avoid very hard or high-organic water that can precipitate components or shift pH/indicator response.
• Sterilization: Standard approach is autoclaving at 121 °C for ~15 minutes (literature). Avoid over-sterilization that can degrade the neutral red indicator.
• Alternatives/adjuncts: Filtration is generally not suitable for media with complex nutrients; autoclaving is preferred.

For experimental needs requiring different selectivity/differential features, consider alternative media rather than changing the solvent.

Storage and Reconstitution

• Storage conditions (item-specific): Room temperature (from Product Data). Keep tightly closed in a cool, dry place. Protect from light to preserve indicator stability. Avoid moisture uptake.
• Shipping: Normal (from Product Data).
• Shelf life: Not specified for this item; refer to CoA/Spec Sheet and product label.
• Reconstitution (general, literature):
  • Use purified/distilled water. Typical working strength is approximately 30–40 g/L, but follow the label for this specific product.
  • Dissolve with heat to near-boiling; mix until fully solubilized.
  • Sterilize by autoclaving at 121 °C for ~15 minutes. Do not over-sterilize to avoid indicator degradation.
  • Allow to cool; verify pH at 25 °C is within the acceptable range per label/SOP.
• In-use handling:
  • Store prepared broth at 2–8 °C if not used immediately; minimize storage time to preserve performance (time limits per SOP).
  • Warm to room temperature before use; do not repeatedly heat/cool.
• Re-freeze/Freeze–thaw: Not applicable; do not freeze dehydrated powder. Avoid repeated freeze–thaw of prepared media.

For precise preparation mass, target pH, and performance criteria, consult the lot-specific CoA/Spec Sheet and follow your validated SOPs. For research use only (from Product Data).

Structure and Identity

MacConkey Broth (EP) is a complex, dehydrated microbiological medium formulated to the European Pharmacopoeia (EP) requirements. It is a mixture, not a single defined chemical compound; therefore, it has no single molecular formula, molecular weight, SMILES, or InChIKey.

• Product type: Dehydrated selective and differential culture medium (mixture)
• EP status: Formulated to meet European Pharmacopoeia criteria (general informational note; verify lot-specific compliance via CoA)
• Molecular formula: Not applicable (mixture)
• Molecular weight: Not applicable (mixture)
• SMILES / InChI / InChIKey: Not applicable (mixture)
• Structural features (general, literature): Contains complex organics (peptones), carbohydrates (typically lactose), selective agents (bile salts, often crystal violet depending on formulation), electrolytes (NaCl), and a pH indicator (commonly neutral red). These components confer selectivity for Gram-negative enteric bacteria and differentiate lactose fermenters.
• 2D structure (descriptive): Heterogeneous blend of low– to medium-molecular-weight organic and inorganic components; no single defined structure.

Item-specific identifiers (MF/MW/SMILES/InChIKey): Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Not applicable to chemical synthesis. MacConkey Broth (EP) is a complex microbiological medium and is not employed as a reagent, catalyst, or solvent in organic or inorganic synthetic transformations. For laboratory synthesis needs, refer instead to appropriate pure reagents, catalysts, and solvent systems.

Target Specificity

Not applicable. This product is not an antibody, probe, or targeted biochemical. It is a selective/differential culture medium mixture that enriches for bile-tolerant Gram-negative enteric bacteria and differentiates lactose fermenters in a functional sense (literature), but it has no molecular target specificity in the immunological or receptor-binding sense.

Perguntas frequentes

How should this product be stored?

Store at room temperature.