Mometasone-d , CAS No.M1434110

CAS: M1434110 Cat. No.: M1434110 Fórmula: C₂₂H₂₃D₅Cl₂O₄ Peso molecular: 432.39

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Visão geral

Mometasone-d 5 is the deuterium labeled Mometasone.

Specifications

Condições de armazenamento de armazenamento

Store at -20°C

Enviado em

Ice chest + Ice pads

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Nomes e identificadores

• Peso molecular: 432.39

Application Protocols

Tested applications, recommended dilutions, or protocols are not provided for this item.

• Application details: Not specified for this item; refer to CoA/Spec Sheet.

General note: For LC–MS/MS workflows, follow your laboratory’s validated method for internal standard preparation, calibration curve construction, QC levels, and acceptance criteria. Do not substitute protocols without appropriate revalidation.

Biological Roles

Item-specific biological data are not provided for this product. The following describes the parent chemotype in general terms.

General (literature):

• Mometasone is a synthetic corticosteroid that functions as a ligand of the glucocorticoid receptor (GR) in biochemical systems, modulating transcriptional responses. Deuteration does not typically alter receptor binding qualitatively, but provides a mass shift for analytical discrimination.
• In research settings, deuterated analogues are used to track distribution, binding, and metabolism in vitro, without altering the fundamental pharmacology of the parent beyond minor kinetic isotope effects.

Practical research implications:

• When used as an internal standard, the deuterated analogue should co-elute with the unlabeled analyte under your chromatographic conditions; confirm co-elution and stable isotope behavior during method validation.
• Evaluate potential deuterium–hydrogen exchange in aqueous buffers, acids, or at elevated temperatures if labeling resides on exchangeable positions; design experiments to avoid artifactual isotope scrambling.

No clinical or therapeutic claims are made. This product is for laboratory research use only, as stated in the Research Use Note.

Buffer Applications

This compound is not a buffer reagent. It is not typically used to establish or maintain pH.

Practical note (general):

• For biochemical assays requiring this analyte/standard in aqueous systems, prepare concentrated stocks in DMSO, ethanol, or methanol and dilute into the desired buffer (e.g., PBS, HEPES) while maintaining a low final organic content (typically ≤1% v/v) to avoid precipitation and biological perturbation.
• If adsorption to plastics is observed (common with lipophilic steroids), consider low-binding tubes, glass vials, or inclusion of carrier proteins/surfactants at low levels compatible with the assay.

Green Alternatives

Greener handling for steroidal analytical standards focuses on solvent selection, waste minimization, and right-sizing experiments. Item-specific constraints are not provided; adopt the following general practices.

• Prefer greener solvents when feasible:
  • Ethanol over methanol where method sensitivity allows (lower toxicity, renewable sources available).
  • Aqueous-organic mobile phases with higher water content while maintaining chromatographic performance.
  • Replace DMF with acetonitrile or ethanol for solution prep when compatible.
• Minimize DMSO volumes: DMSO is effective but persistent; keep final assay concentrations as low as practicable (e.g., ≤0.5–1% v/v) to reduce environmental and biological impact.
• Microscale workflows: Deuterated reagents are resource-intensive. Calibrate balances for sub-milligram weighing, use low-dead-volume vials, and adopt microtiter formats to reduce consumption and waste.
• Closed transfers: Use positive-displacement pipettes or glass syringes to avoid loss and volatilization of organic solvents.

Illustrative comparison (general guidance):

• Methanol vs ethanol for stock solutions: ethanol offers lower toxicity and greener sourcing; methanol often gives slightly higher solubility and is more volatile (easier drying). Choose based on method performance and safety policy.

Implement routine solvent recycling where available and segregate halogenated from non-halogenated waste to facilitate downstream treatment.

Pharmaceutical Uses

No excipient or pharmacopeial status is specified for this item; this product is supplied for research use only.

Relevant non-clinical uses (general):

• Reference and system-suitability standard in method development and QC of analytical procedures aimed at quantifying mometasone (or related steroids) in development samples, stability studies, or process streams.
• Calibration of extraction efficiency and matrix effects in bioanalytical workflows via isotope-dilution techniques.
• Cross-validation of chromatographic identity by co-injection with unlabeled material to confirm retention time and fragmentation patterns.

If your quality system requires traceability, request documentation of isotopic enrichment, purity, and analytical characterization from the CoA and maintain chain-of-custody records. This product is not intended for human or veterinary use, drug substance manufacture, or diagnostic applications.

Physical Properties

Item-specific constants:

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not applicable/rarely reported for nonvolatile steroids; item-specific value not specified.
• Density, refractive index, pKa, logP, UV cutoff: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

General characteristics (literature, for corticosteroid analogues):

• Physical state: typically crystalline solids with low volatility.
• Solubility tendencies: sparingly soluble in water; soluble in polar organic solvents such as DMSO, DMF, methanol, ethanol, and acetonitrile; solubility often improved with co-solvents or surfactants.
• Lipophilicity: moderate to high, consistent with steroidal frameworks; expect good partitioning into organic phases.

Practical handling implications (general):

• Prepare concentrated stock solutions in anhydrous DMSO or methanol for analytical work; then dilute into aqueous media with ≤1% organic cosolvent to maintain solubility while minimizing matrix effects.
• Avoid prolonged exposure to strong light or heat; many oxygenated steroids are photosensitive and may slowly oxidize (general consideration). Always verify stability and solution shelf life using your own method conditions.

Quality and Grades

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.

Context for deuterated analytical standards (general guidance):

• Isotopic labeling: For LC–MS/MS internal standards, isotopic enrichment (e.g., %D incorporation and labeling positions) is critical to ensure co-elution and mass separation from the analyte while avoiding back-exchange. Request and retain the isotopic distribution data and labeling map from the CoA.
• Chemical purity vs. isotopic purity: Both matter. Chemical purity affects chromatographic background and ion suppression; isotopic purity dictates internal-standard performance. Specifications typically include assay (%), related substances, residual solvents, and water content—obtain these from the CoA.
• Identity confirmation: Vendors commonly provide 1H/13C NMR (with attenuated 1H signals at labeled positions), HRMS/LC–MS, and sometimes IR. For deuterated materials, qNMR against a calibrated standard or mass-balance purity by LC are frequently used.
• Stabilizers: Not specified for this item. If a stabilizer is present (e.g., antioxidant), it will be noted on the CoA; assess compatibility with your assay.
• Fit-for-purpose: For quantitative bioanalysis, look for documentation on stability in common solvents (DMSO, MeOH, ACN), bench-top/injector stability, and freeze–thaw robustness. If using as a system suitability or reference standard, ensure traceability where required by your QA system.

Reaction and Applications

This product is best positioned as a deuterated analogue for analytical and mechanistic studies rather than as a bulk reagent for synthesis. Item-specific application validation is not provided; the following are common literature uses for deuterated corticosteroid standards.

Analytical applications (literature/general):

• Internal standard for LC–MS/MS quantitation of mometasone (or related analogues), enabling isotope-dilution accuracy with matched extraction recovery and matrix effects.
• Recovery and process control in sample preparation (protein precipitation, SPE, LLE) from complex matrices.
• Stability-indicating method development: monitoring degradation pathways by tracking labeled vs unlabeled signals.

Mechanistic and biotransformation studies (literature/general):

• Tracer for metabolic fate mapping (e.g., phase I/II transformations) where deuterium labeling supports identification of specific fragmentation routes by MS/MS.
• Kinetic isotope effect probing at labeled positions when the labeling site overlaps with the reaction coordinate (if known; labeling pattern for this item is not specified).

Practical tips:

• Prepare fresh working solutions in LC–MS-grade solvents; filter mobile phases (0.2 µm) to reduce background.
• Match the organic composition of the internal standard solution to that of calibration and sample extracts to minimize differential ionization.
• Verify absence of deuterium–hydrogen back-exchange under your conditions (avoid strong acids/bases and high temperatures if labeling is labile).

Reaction Conditions

Item-specific reactivity data are not provided. The following general conditions relate to preparing, handling, and analyzing deuterated steroidal standards.

Stock solution preparation (general):

• Dissolve accurately weighed material in anhydrous DMSO, methanol, ethanol, or acetonitrile to prepare 0.1–10 mg/mL stocks as suited to your assay sensitivity. Filter through a PTFE syringe filter if particulate is visible.
• Store aliquots in amber vials at −20 °C or colder to minimize degradation; avoid repeated freeze–thaw cycles.

LC–MS/MS method development (general):

• Mobile phases: Water with 0.1% formic acid (or ammonium formate/acetate) and acetonitrile or methanol organic phase. Gradient elution commonly improves peak shape for lipophilic steroids.
• Ionization: ESI+ or APCI+ are often effective for corticosteroids; optimize source temperature and gas flows to balance sensitivity against in-source fragmentation.
• Transitions: Select MRM transitions offset by the deuterium mass shift relative to the unlabeled analyte; verify absence of cross-talk.

Stability considerations (general):

• Avoid strong acids/bases and prolonged light exposure. Assess bench-top, autosampler (4–10 °C), and freeze–thaw stability during validation.

All numeric ranges above are illustrative literature practices, not item-specific specifications. Validate conditions under your laboratory’s QA procedures.

Safety and Handling

GHS and hazard details for this specific item:

• Signal word: Not specified for this item; refer to SDS.
• H-statements: Not specified for this item; refer to SDS.
• GHS classification and pictograms: Not specified for this item; refer to SDS.

General laboratory safety guidance (deuterated corticosteroid standard):

• Potential hazards: Steroidal compounds can exhibit potent biological activity. Avoid inhalation of dust, skin contact, and accidental ingestion. Handle powders in a ventilated enclosure or fume hood; use anti-static tools to minimize aerosolization.
• PPE: Wear lab coat, safety glasses, and appropriate gloves (e.g., nitrile). For weighing fine powders, consider double-gloving and using a dust mask or respirator per institutional policy.
• Engineering controls: Work in a certified chemical fume hood or balance enclosure. Use sealed, labeled containers; minimize open handling time.
• Incompatibilities (general): Avoid strong oxidizers and strong bases/acids that may promote degradation. Use dry, oxygen- and light-limiting conditions for long-term stability of stock solutions.
• First aid (overview; defer to SDS): If on skin, wash with soap and water. If in eyes, rinse carefully with water for several minutes. If inhaled, move to fresh air. If ingested, rinse mouth. Seek medical attention as appropriate and present SDS.
• Waste: Collect solid and solution waste in appropriate organic waste streams; decontaminate surfaces with compatible solvent and detergent.

Always consult the product-specific SDS for authoritative hazard, toxicology, and response information.

Solvent Selection

Item-specific solubility data are not provided for this product. The following guidance reflects general behavior of steroidal small molecules and deuterated analytical standards.

• Polarity profile: Steroidal frameworks are lipophilic with limited aqueous solubility; they dissolve readily in polar organic solvents capable of hydrogen-bond acceptance/donation.
• Preferred solvents for stock solutions (general):
  • DMSO (anhydrous): excellent solvating power; stable, miscible with water at low percentages.
  • Methanol or ethanol: good solubility for many corticosteroids; convenient for LC–MS.
  • Acetonitrile: useful for LC–MS, often with a small water fraction to match mobile phase.
• Aqueous compatibility: For biochemical assays, dilute organic stocks into buffer such as PBS with 0.1–1% DMSO (v/v) to limit precipitation. Gentle vortexing and brief sonication can help.
• Avoid:
  • Neat water additions to solid material—risk of aggregation/precipitation.
  • Highly basic aqueous solutions that may promote degradation of oxygenated steroids.

Comparison (general):

• DMSO vs MeOH: DMSO affords highest solubility and stability but can affect biological assays above ~0.5–1% (v/v). MeOH is more volatile, easier to remove, but can influence protein conformation at higher levels. Choose based on downstream needs (bioassay vs analytical injection).

Validate solvent choice empirically under your method conditions and document solution stability for your specific workflows.

Storage and Reconstitution

Item-specific storage and shipping:

• Storage conditions: Store at −20 °C.
• Shipped in: Ice chest + ice pads.

General best practices for this product class:

• Protect from light and moisture. Keep container tightly closed in a desiccated environment. Consider secondary containment with desiccant.
• Reconstitution: Prepare concentrated stocks in anhydrous DMSO, methanol, ethanol, or acetonitrile. Vortex thoroughly and, if needed, briefly sonicate to ensure complete dissolution.
• Aliquoting: Dispense single-use aliquots in amber glass vials to avoid adsorption and repeated freeze–thaw. Clearly label with concentration, solvent, and preparation date.
• Solution storage: Store organic stock solutions at −20 °C (short to mid-term) or −80 °C (long-term), monitoring for precipitation or degradation. Allow solutions to equilibrate to room temperature before opening to prevent moisture ingress; mix before use.
• Stability: Item-specific stability data are not provided. Verify stability under your conditions (bench-top, autosampler, freeze–thaw) during method development and retain data in your records.

Research use only: Not for human or veterinary use. Refer to the CoA/SDS for any additional handling or storage constraints specific to this lot.

Structure and Identity

• SKU: M1434110
• Product name: Mometasone-d
• CAS: M1434110 (catalog identifier)
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

General structural description (literature):

• Mometasone is a synthetic corticosteroid (steroidal scaffold with four fused rings: cyclopentanoperhydrophenanthrene core) bearing multiple oxygenated substituents. “Mometasone-d” denotes a deuterium-labeled analogue in which one or more hydrogens are replaced by deuterium; the exact labeling pattern and count are not specified for this item.
• Functional group class (parent compound, literature): secondary/tertiary alcohols and carbonyl functionality typical of glucocorticoids; halogenation may be present in some mometasone derivatives (literature). The precise functionalization for this deuterated item is not specified.

Notes:

• Because the isotopic labeling pattern and analytical identifiers are not provided here, consult the product CoA or specification sheet for definitive identity attributes required for regulatory or analytical documentation.

Synthetic Utility

Although primarily used as an analytical standard, a deuterated mometasone analogue can be valuable in mechanistic and methodological chemistry.

General utilities (literature):

• Kinetic isotope effect (KIE) probes: If deuterium labels reside at mechanistically relevant C–H sites, comparative rates or isotope-sensitive product distributions can inform reaction pathways involving hydrogen transfer or oxidation on the steroid framework.
• Tracer in complex reaction networks: Distinguish competing oxidative, reductive, or rearrangement pathways on oxygenated steroids by MS, leveraging mass shifts from deuterium.
• Method development: Validate derivatization efficiency (e.g., oxime formation on carbonyls for GC analysis) using a labeled analogue to benchmark conversion and recovery.

Practical considerations:

• Label integrity: Avoid protic acids/bases and elevated temperatures that can promote H/D exchange at activated positions (allylic/benzylic, α-to-carbonyl, or heteroatom-bound sites).
• Workup: Use mild, neutral quench and cold conditions where possible to preserve labels. Perform rapid extractions with degassed organic solvents and minimize aqueous contact.

Note: The precise deuteration pattern for this item is not specified; applicability to a given mechanistic question depends on where deuterium is incorporated. Consult the CoA for labeling positions before planning experiments.

Target Specificity

• Target/antigen, clone, isotype, and species reactivity: Not applicable to this small-molecule product.
• Item-specific target-binding data: Not specified for this item; refer to CoA/Spec Sheet if any affinity data are provided.

Note: As a deuterated small molecule, this product is typically used as an analytical internal standard rather than a target-specific binder.