Mycoplasma Staining Solution (DNA Fluorescent Staining Method) - BioReagent, Suitable for microbiology, Biological Stain, for Cell culture, for Microscopy

Cat. No.: M1524666

GRADE & PURITY BioReagent, Suitable for microbiology, Biological Stain, for Microscopy, for Cell culture

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

Cell Culture, Microbial staining

Visão geral

Mycoplasma Staining Solution (DNA Fluorescent Staining Method) is a reagent for in‑situ staining to detect mycoplasma or other prokaryotes in cultured cells. It is mainly used to verify mycoplasma contamination in cultured cells.

Bacterial, yeast and mold contamination in cultured cells can be observed under an optical microscope. However, mycoplasma contamination cannot be visualized under an optical microscope and must be examined by dedicated methods. Common mycoplasma detection approaches include mycoplasma isolation and culture, mycoplasma‑specific enzyme assay, RT‑PCR assay, and DNA fluorescent staining. This product detects mycoplasma via DNA fluorescent staining, enabling rapid, effective and highly‑sensitive identification of mycoplasma contamination.

Operating Procedures:

1. Add Mycoplasma Detection Staining Solution at a ratio of 2 drops per mL of culture medium.

2. Incubate at room temperature for 15‑30 minutes.

3. Observe under a fluorescence microscope (wavelength: 360～400 nm).

Case Analysis:

              1. No contamination         2. Mild contamination        3. Severe contamination

Refer to the figure above for reference detection results of this kit.

Figure 1 shows the condition without mycoplasma contamination. Figure 2 shows mild mycoplasma contamination; particulate mycoplasma particles are indicated by arrows. Figure 3 shows severe mycoplasma contamination, where abundant particulate mycoplasma can be observed.

Precautions:

1. This staining reagent is hazardous to human body. Handle with caution and take adequate protective measures to avoid direct physical contact or inhalation.

2. Fluorescent dyes are susceptible to photobleaching. It is recommended to complete detection on the same day after staining.

3. This product is for scientific research use by professionals only. Wear a lab coat and gloves during operation for your safety and health.

Specifications

Especificações e pureza

BioReagent, Suitable for microbiology, Biological Stain, for Cell culture, for Microscopy

Estabilidade e armazenamento

Store at -20°C long term (12 months). Store in the dark. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Condições de armazenamento de armazenamento

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Grau

Biological Stain, BioReagent, for Cell culture, for Microscopy, Suitable for microbiology