novokinin, Agonist of AT 2 receptor, CAS No.rp174652, Agonist of AT 2 receptor

CAS: rp174652 Cat. No.: rp174652
Disponível para encomenda
GRADE & PURITY Moligand™ ? Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.
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Size
Alemanha (EU)
USA*
Price
Qty
500μg
rp174652-500μg
Sob encomenda · 8–12 semanas
2082,49€
1mg
rp174652-1mg
Sob encomenda · 8–12 semanas
3470,87€
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Why this grade

Moligand™ Moligand™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Room temperature Ships Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Specifications

Product Name
novokinin, Agonist of AT 2 receptor, CAS No.rp174652
Grau
Moligand™
Especificações e pureza
Moligand™
Tipo de ação
AGONIST
Mecanismo de ação
Agonist of AT 2 receptor
CAS
rp174652
Tipo de molécula
Peptide
Product Line
Produtos biológicos
Armazenamento e envio
Condições de armazenamento de armazenamento
Room temperature

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Taxonomic Classification

Taxonomy Tree

KingdomOrganic compounds
SuperclassOrganic acids and derivatives
ClasseCarboxylic acids and derivatives
SubclassAmino acids, peptides, and analogues
Intermediate Tree Nodes Peptides
Direct ParentOligopeptides
Alternative Parents Leucine and derivatives  Proline and derivatives  N-acyl-L-alpha-amino acids  Alpha amino acid amides  Indolyl carboxylic acids and derivatives  3-alkylindoles  Pyrrolidinecarboxamides  N-acylpyrrolidines  Substituted pyrroles  N-acyl amines  Benzenoids  Tertiary carboxylic acid amides  Heteroaromatic compounds  Secondary carboxylic acid amides  Amino acids  Guanidines  Propargyl-type 1,3-dipolar organic compounds  Monocarboxylic acids and derivatives  Azacyclic compounds  Carboximidamides  Carboxylic acids  Carbonyl compounds  Organic oxides  Monoalkylamines  Hydrocarbon derivatives  
Molecular FrameworkAromatic heteropolycyclic compounds
Substituents Alpha-oligopeptide - Leucine or derivatives - Proline or derivatives - N-acyl-l-alpha-amino acid - N-acyl-alpha-amino acid - N-acyl-alpha amino acid or derivatives - Alpha-amino acid amide - Indolyl carboxylic acid derivative - N-substituted-alpha-amino acid - Alpha-amino acid or derivatives - 3-alkylindole - Indole - Indole or derivatives - N-acylpyrrolidine - Pyrrolidine carboxylic acid or derivatives - Pyrrolidine-2-carboxamide - Benzenoid - Substituted pyrrole - Fatty amide - N-acyl-amine - Fatty acyl - Tertiary carboxylic acid amide - Pyrrolidine - Pyrrole - Heteroaromatic compound - Amino acid or derivatives - Secondary carboxylic acid amide - Carboxamide group - Guanidine - Amino acid - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Organoheterocyclic compound - Carboximidamide - Carboxylic acid - Monocarboxylic acid or derivatives - Azacycle - Primary amine - Organic oxygen compound - Amine - Organic oxide - Organooxygen compound - Hydrocarbon derivative - Organonitrogen compound - Primary aliphatic amine - Organic nitrogen compound - Carbonyl group - Aromatic heteropolycyclic compound
DescriçãoThis compound belongs to the class of organic compounds known as oligopeptides. These are organic compounds containing a sequence of between three and ten alpha-amino acids joined by peptide bonds.
External Descriptors Not available
Estrutura 3D
Modelo de Estrutura Química Interativa





Alvos associados (humanos)
AGTR2 Tchem Type-2 angiotensin II receptor (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificados(CoA,COO,BSE/TSE e Mapa de Análise)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Informação genética
Reference
  • 1. Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration., The Journal of biological chemistry, Steegborn, C C and 7 more authors.
  • 2. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences., Proceedings of the National Academy of Sciences of the United States of America, Strausberg, Robert L RL and 83 more authors.
  • 3. Genomic basis of cystathioninuria (MIM 219500) revealed by multiple mutations in cystathionine gamma-lyase (CTH)., Human genetics, Wang, Jian J and Hegele, Robert A RA.
  • 4. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase., Biochemical and biophysical research communications, Lu, Y Y, O'Dowd, B F BF, Orrego, H H and Israel, Y Y.
  • 5. Single nucleotide polymorphism in CTH associated with variation in plasma homocysteine concentration., Clinical genetics, Wang, J J, Huff, A M AM, Spence, J D JD and Hegele, R A RA.
  • 6. Cystathionine gamma-lyase overexpression inhibits cell proliferation via a H2S-dependent modulation of ERK1/2 phosphorylation and p21Cip/WAK-1., The Journal of biological chemistry, Yang, Guangdong G, Cao, Kun K, Wu, Lingyun L and Wang, Rui R.
  • 7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)., Genome research, Gerhard, Daniela S DS and 115 more authors.
  • 8. Towards a proteome-scale map of the human protein-protein interaction network., Nature, Rual, Jean-François JF and 37 more authors.
  • 9. The DNA sequence and biological annotation of human chromosome 1., Nature, Gregory, S G SG and 178 more authors.
  • 10. Polymorphisms in one-carbon metabolism and trans-sulfuration pathway genes and susceptibility to bladder cancer., International journal of cancer, Moore, Lee E LE and 14 more authors. more
Calculadoras de soluções
Revisões

Avaliações dos Clientes

Application Protocols

No item-specific, tested application protocols are provided for this SKU.

General workflow suggestions for peptidic ligands (literature/general; adapt to your assay):

  • Preparation: Equilibrate vial to room temperature before opening to avoid moisture condensation. Dissolve in water or minimal DMSO, then dilute with assay buffer.
  • Concentration–response: Prepare serial dilutions (e.g., 1:3 or 1:10) to span a wide range; include vehicle controls to quantify solvent effects.
  • Binding assays: For radioligand or fluorescence polarization/BRET assays, pre-incubate ligand with target at defined temperature (e.g., 25–37°C) until equilibrium; determine non-specific binding using excess competitor.
  • Functional assays: In GPCR readouts (e.g., cAMP, ERK, calcium), pre-equilibrate cells in assay buffer, minimize serum, and apply ligand across a dilution series; quantify with appropriate detection kits.
  • Data quality: Employ low-bind consumables, assess adsorption losses, and include stability checks (re-assay potency after bench-top exposure).

For any validated, item-specific protocols or recommended dilutions, refer to the CoA/Spec Sheet.

Biological Roles

Biological role information here is provided as general literature context and not as a claim about this specific lot or for clinical use.

  • Literature reports describe novokinin-type peptides as kininogen-derived sequences studied as ligands/agonists of the angiotensin II type 2 receptor (AT2R), contributing to investigations of the renin–angiotensin and kallikrein–kinin systems.
  • As a peptidic ligand, novokinin is used to probe receptor-mediated signaling cascades (e.g., modulation of GPCR pathways) in cell-based or biochemical assays (binding, second-messenger readouts). Exact potency, selectivity, and sequence can vary by source and experimental conditions; consult primary references and verify with your own controls.
  • Peptide ligands commonly exhibit susceptibility to proteolysis; inclusion of protease inhibitors or use in serum-free systems may be necessary to preserve activity in biological assays (general guidance).
  • Binding interactions are often salt- and pH-sensitive; experimental buffers should be optimized for ionic strength and pH to reflect physiological conditions while maintaining ligand stability.

Item-specific biological data (affinity, EC50/IC50, selectivity profile) are Not specified for this item; refer to CoA/Spec Sheet or generate de novo under your assay conditions.

Buffer Applications

This product is a peptidic ligand and not a buffering agent. Therefore, it is not typically used to establish or maintain pH.

Practical notes (general/literature for handling peptide ligands in buffers):

  • Dissolution: Start with water or assay buffer; adjust pH slightly if needed to enhance solubility (avoid extremes that may hydrolyze peptide bonds).
  • Common assay buffers: PBS, HBSS, HEPES-buffered saline; include 0.1% BSA or 0.01% Tween-20 if adsorption losses are observed and if compatible with the assay.
  • Filtration: 0.22 µm filtration may be used to sterilize solutions; confirm peptide recovery.

Item-specific buffer compatibility and pH stability are Not specified for this item; refer to CoA/Spec Sheet or determine empirically.

Green Alternatives

Green chemistry alternatives typically apply to solvents and commodity reagents. For a peptidic screening ligand like novokinin, the concept of a “green alternative” is not directly applicable.

General sustainability considerations for peptide ligands (literature/general):

  • Use aqueous buffers whenever feasible instead of high percentages of organic cosolvents.
  • Minimize DMSO content in working solutions (≤0.1–1% v/v) to reduce solvent waste and assay interference.
  • Prepare only the quantities needed for immediate experiments to limit freeze–thaw cycles and disposal.
  • Employ micro-scale assays and 96/384-well formats to reduce chemical consumption and waste.

If your workflow requires organic solvents (e.g., initial dissolution), consider:

  • Water/buffer > ethanol > isopropanol ≫ DMSO in terms of general environmental profiles, acknowledging that DMSO is often necessary for hydrophobic content but is relatively benign compared with halogenated solvents.

Note: These points are general guidance and not item-specific specifications.

Pharmaceutical Uses

This product is supplied strictly for research use only. No clinical or therapeutic use is intended or implied.

Formulation-related context (general literature for peptide ligands; not item-specific):

  • In preclinical research settings, peptidic ligands may be formulated for in vitro assays in aqueous buffers or as DMSO concentrates for subsequent dilution.
  • For non-clinical in vivo studies (outside the scope of this product listing), typical considerations include isotonicity, pH adjustment, and avoidance of adsorption to containers; however, such applications require additional qualification and are not covered by this product’s research-use-only designation.
  • Pharmacopeial status, excipient compatibility, sterility/pyrogen testing, and GMP documentation are Not applicable to this research-grade Moligand™ product.

Item-specific excipient content, counterions, and sterility status: Not specified for this item; refer to CoA/Spec Sheet.

Physical Properties

Item-specific physicochemical parameters are not listed for this SKU.

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Melting point: Not specified for this item; refer to CoA/Spec Sheet.
  • Solubility: Not specified for this item; refer to CoA/Spec Sheet.
  • LogP, pKa, density, refractive index: Not specified for this item; refer to CoA/Spec Sheet.

General literature guidance for peptidic ligands (not specifications):

  • Solubility is typically good in aqueous buffers at neutral to slightly acidic pH and in polar aprotic solvents such as DMSO; initial dissolution in minimal DMSO followed by aqueous dilution is a common approach.
  • Many peptides are hygroscopic and can form gels or foams on rapid addition of water; gentle swirling and stepwise dilution can help.
  • Salt form (e.g., TFA vs acetate) can influence apparent pH and solubility; verify on the CoA.
  • Typical peptide solutions are prepared at 1–10 mM in DMSO or water and then diluted to working concentration with buffer (literature/general).
Quality and Grades
  • Grade/Purity designation: Moligand™ (as provided for this SKU).

What Moligand™ implies (general description):

  • Moligand™ denotes a screening-grade small-molecule/ligand entry in a discovery library context, intended for biochemical or cellular target engagement studies under research-use-only terms. This typically prioritizes identity and functional utility for screening over exhaustive trace-metal/UV-cutoff specifications.

Item-specific notes:

  • Purity level, salt form, counterions, and residual solvents are Not specified for this item; refer to CoA/Spec Sheet.
  • Stabilizers or additives: Not specified for this item; refer to CoA/Spec Sheet.

Practical implications:

  • For screening/assay work: verify identity by LC–MS and purity by HPLC where your application requires stringent thresholds (e.g., biochemical Kd measurements vs. phenotypic screens).
  • For SAR or medicinal chemistry follow-up: consider re-purification or orthogonal analytics to confirm counterion content and water content, which can influence assay potency on a mass basis.
  • Documentation: Request the lot-specific CoA/SDS to confirm salt form, peptide content (%), water content, and any residual TFA/acetate that may affect biological assays.
Reaction and Applications

This product is positioned as a Moligand™ screening ligand rather than as a synthetic reagent or solvent. As such, classical organic reactions (e.g., Grignard, cross-couplings) are not typical use cases for this item.

Research application contexts (literature/general for novokinin-like peptides):

  • Employed as a tool ligand in receptor-binding and functional assays to interrogate signaling pathways related to the renin–angiotensin and kinin systems, particularly AT2 receptor engagement.
  • Utilized in target validation studies, assay development (e.g., calcium flux, cAMP, ERK phosphorylation), and phenotypic screening where peptide ligands serve as positive controls or probes.

Practical tips for assay deployment (general):

  • Prepare concentrated stocks (e.g., 1–10 mM in DMSO or water) and perform serial dilutions freshly to minimize adsorption and hydrolysis.
  • Include carrier protein (0.1% BSA) or low levels of surfactant (e.g., 0.01% Tween-20) in assay buffer to reduce non-specific binding where compatible with readout.
  • Use low-bind plasticware and pre-rinse pipette tips to mitigate peptide loss at low nanomolar concentrations.

If you intend to derivatize or immobilize the ligand (e.g., for affinity matrices), verify the presence/availability of functional handles (N-terminus, side-chain amines/carboxyls) on the specific lot via the CoA; item-specific sequence information is Not specified for this item.

Reaction Conditions

Classical reaction-condition guidance is not directly applicable because this product is a peptidic ligand rather than a synthetic reagent. For biochemical and cell-based assay usage, the following general, literature-based handling recommendations apply (not item-specific specifications):

  • Stock solutions: 1–10 mM in water or DMSO; filter-sterilize if required for cell assays; store aliquots to minimize freeze–thaw (see Storage tab for item-specific storage conditions).
  • Working concentrations: Determined empirically by assay type; typical GPCR ligand studies explore 0.1 nM to 10 µM ranges to generate full concentration–response curves.
  • Buffers: Physiological ionic strength (e.g., HBSS, PBS, HEPES-buffered media) at pH 7.2–7.4; include protease inhibitors if stability is a concern.
  • Temperature/time: Equilibration times can range from minutes (functional responses) to hours (binding equilibrium), commonly at 25–37°C depending on the assay system.

Item-specific potency, kinetic parameters, and stability under given conditions are Not specified for this item; validate under your experimental setup.

Safety and Handling

Safety data for this specific item are not provided in the listing. Treat as a laboratory chemical/biomolecule and consult the SDS for authoritative guidance.

  • GHS classification: Not specified for this item; refer to SDS.
  • Signal word / H-statements / pictograms: Not specified for this item; refer to SDS.

Good laboratory practice (general):

  • PPE: Use lab coat, nitrile gloves, and safety glasses. Handle powders/lyophilized solids in a fume hood or containment to avoid inhalation of dust/aerosol.
  • Avoid ingestion and inhalation. Prevent contact with skin and eyes. Wash thoroughly after handling.
  • Incompatibilities (general peptide materials): strong oxidizers and strong acids/bases may lead to degradation (hydrolysis, side-chain reactions). Avoid prolonged exposure to elevated temperatures.
  • First-aid overview (general): If inhaled, move to fresh air; if on skin, wash with soap/water; if in eyes, rinse cautiously with water for several minutes; if ingested, rinse mouth—seek medical attention in all cases and provide the SDS to responders.
  • Environmental note: Avoid release to the environment. Dispose according to institutional and local regulations.

Always defer to the product-specific SDS and institutional risk assessments.

Solvent Selection

No item-specific solubility data are provided. The following guidance reflects general practice for peptidic ligands (literature/general) and should be validated empirically.

  • Polarity class: Highly polar, hydrogen-bonding (typical for peptides; may carry ionizable groups).
  • Common solvents: Water, buffered saline (e.g., PBS), dilute acids (0.1% TFA or acetic acid) to aid initial dissolution; DMSO for stock solutions if aqueous solubility is limited.
  • Miscibility profile: DMSO is miscible with water and supports concentrated stock (e.g., 10–50 mM) that can be diluted into assay buffer with final DMSO ≤0.1–1% v/v to minimize biological interference (literature/general).

Selection tips:

  • Start with high-purity water or buffer at working pH. If insoluble, wet the material with a minimal volume of DMSO, then stepwise add aqueous buffer while gently mixing.
  • Adjust pH toward the side favoring ionization of the most basic/acidic residues (e.g., mildly acidic conditions for cationic peptides) to increase solubility.
  • Filter sterilization (0.22 µm) may be used for cell-based assays if the peptide is stable; confirm recovery to avoid peptide loss on membranes.

Comparison (general):

  • Water/buffer: biologically compatible, best first choice.
  • DMSO: maximizes solubility for hydrophobic motifs but may affect assays at higher %.
  • Alcohols (MeOH, EtOH): less favored; can denature proteins in biochemical assays and provide limited solubility gain for charged peptides.
Storage and Reconstitution
  • Storage conditions (as provided): Room temperature.
  • Shipped in: Not specified for this item; refer to CoA/Spec Sheet.
  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.

Reconstitution and handling (general literature guidance; not item-specific specifications):

  • Allow vial to reach room temperature in a desiccated environment before opening to minimize moisture uptake.
  • For initial dissolution, use sterile water or minimal DMSO, then dilute with buffer to the desired working concentration. Gentle mixing helps avoid foaming.
  • Prepare single-use aliquots to avoid repeated freeze–thaw. If freezing is necessary for your workflow, store at ≤−20°C in low-bind tubes and protect from light if the peptide contains photo-labile residues or labels.
  • Stability of solutions depends on sequence and buffer; assess by LC–MS or HPLC after storage. Incorporate protease inhibitors where compatible.

Note: Where stability, shelf-life, and re-test dates are critical, rely on the lot-specific CoA and SDS. Any parameters not listed above are Not specified for this item; refer to CoA/Spec Sheet.

Structure and Identity

Novokinin is cataloged here as a small-molecule/peptidic ligand for research screening. Item-specific structural identifiers are largely not provided in this listing.

  • Product name: novokinin (research ligand)
  • CAS: rp174652 (catalog placeholder; not a standard CAS format)
  • InChIKey: 394691 (as provided; not a standard InChIKey string)
  • SMILES: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general literature context):

  • Novokinin is reported in the literature as a short peptide ligand derived from kininogen motifs and studied as an agonist at the angiotensin II type 2 receptor (AT2R). Exact amino acid sequence and salt form may vary by source; consult the specific CoA for this item.
  • Peptidic ligands typically contain multiple amide bonds, basic/acidic side chains, and may be supplied as trifluoroacetate (TFA) or acetate salts (literature/general).

2D description in words (general peptide features):

  • Linear oligomer of alpha-amino acids joined by amide (peptide) linkages, with terminal amino and carboxyl functions (or protected/blocked termini depending on lot/form; not specified here).
Synthetic Utility

As a peptidic screening ligand, novokinin is not typically used as a building block in routine organic synthesis. Nevertheless, peptides possess functional groups that can be leveraged for conjugation or surface immobilization in chemical biology workflows.

General synthetic/conjugation considerations (literature/general):

  • Functional handles: N-terminus (amine), C-terminus (carboxylate), and nucleophilic side chains (Lys, Cys, Ser, Tyr) enable coupling via NHS ester amide formation, maleimide–thiol chemistry, click reactions (after azide/alkyne installation), or enzymatic ligations.
  • Biophysical tool generation: Attachment of fluorophores, biotin, or affinity tags (e.g., NHS–biotin) to create probes for pull-downs, BLI/SPR, or fluorescence assays.
  • Solid supports: Coupling to resins or sensor chips (e.g., EDC/NHS activation on carboxymethyl dextran) for kinetic binding analysis.

If derivatization is planned, verify sequence and terminal protection state on the specific lot; item-specific structural details are Not specified for this item and should be confirmed via CoA or analytical characterization (LC–MS, peptide mapping).

Target Specificity

Target information is not provided in the product data for this SKU.

  • Antigen/target, receptor subtype, species selectivity, clone/isotype (if antibody), and validated off-target profiles: Not specified for this item; refer to CoA/Spec Sheet.

General literature context:

  • Novokinin has been studied as a peptidic ligand interacting with the angiotensin II type 2 receptor (AT2R). Exact affinity, selectivity versus AT1R or other receptors, and species dependence vary by source and experimental conditions. Users should determine specificity within their own assay systems using appropriate positive/negative controls.

Perguntas frequentes

What is Moligand??
Moligand? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, Moligand? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.
What are the CAS number, molecular formula and molecular weight?
The CAS Number is rp174652. InChIKey WCJSVACAALWGRF-LQPYQXOBSA-N.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

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