GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple prote
Espécie hospedeira
Mouse
Especificidade
PCNA
Imunogénio
Full length recombinant human PCNA
Controlo positivo
IF/ICC: Hela cells Flow Cytometry: Hela cells
Conjugação
TRITC
Excitação (nm)
Green Laser(532nm)
Emissão (nm)
576nm
Ex/Em(nm)
Ex:557nm;Em:576nm
Grau
ExactAb™, Validated
Tipo de corrente de luz
kappa
Método de purificação
Protein A purified
Concentração
0.5 mg/mL
Armazenamento e envio
Condições de armazenamento de armazenamento
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Enviado em
Ice chest + Ice pads
Estabilidade e armazenamento
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Imagens
PCNA Mouse mAb (TRITC) (Ab176325) - ICC/IF IF analysis of PCNA (Red) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (TRITC) (Ab176325) at a dilution of 1/1000 in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
PCNA Mouse mAb (TRITC) (Ab176325) - Flow Cytometry Flow cytometry analysis of PCNA expression by Hela cells (Red) compared to an isotype control (Blue). Cells were fixed and permeabilized with 100% methanol and then stained with PCNA Mouse mAb (TRITC) (Ab176325) (Red) at 1/50 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (TRITC) (Ab225743).
Aplicação
Aplicação
Dilution info
IF/ICC
1:50-1:500
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Dilution Calculator
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Reconstitution Calculator
Revisões
Avaliações dos Clientes
Perguntas frequentes
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Validated mean?
Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How should this product be stored?
Store at ?20 °C, protected from light. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PCNA Mouse mAb (TRITC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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