Peptone Sodium Chloride Solution - BioReagent

Cat. No.: P1528259

GRADE & PURITY BioReagent

Storage

Store at 2-8°C

Shipped In

Wet ice

Visão geral

For sample preparation in microbiological testing of Staphylococcus and other microorganisms

Specifications

Especificações e pureza

BioReagent

Condições de armazenamento de armazenamento

Store at 2-8°C

Enviado em

Wet ice

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Grau

BioReagent

Product Line

Produtos biológicos

Application Protocols

No item-specific validated protocols or tested applications are provided in the Product Data. Refer to your governing method (e.g., ISO, USP, BAM) and internal SOPs. General, literature-based examples include:

• Serial dilutions for plate counts:
  • Prepare a sterile working area. Transfer 1 mL of sample into 9 mL of solution to obtain 10⁻¹ dilution. Vortex to mix. Repeat serially to desired dilution. Plate appropriate volumes on selective/non-selective agar. Incubate per organism requirements.
• Surface or swab sampling:
  • Moisten sterile swab with solution, sample defined area, place swab into tube containing solution, vortex, and perform dilutions/plate counts.
• Filter or device rinse:
  • Rinse filters or small parts with measured volumes to elute microorganisms, then assay eluate per method.

These are general-use illustrations; validate parameters (volumes, times, temperatures, incubation conditions) according to your SOPs. Sterility, pH, and composition should be verified from the CoA/Spec Sheet for regulated work.

Biological Roles

General/biochemistry context (not product specifications):

• Peptone is a complex mixture of peptides and amino acids produced by proteolysis of proteins (e.g., casein, meat, soy). It supplies nitrogen, carbon, and growth factors in many microbiological media. In dilute form within saline, it helps maintain microbial viability during short handling steps without promoting extensive growth.
• Sodium chloride provides osmotic balance, maintaining cell turgor and preventing lysis or plasmolysis during dilution, washing, or transport. An isotonic environment is critical for maintaining membrane integrity of many bacteria.
• Compared with saline alone, trace peptides and amino acids can mitigate stress responses and improve recovery of injured or environmentally challenged cells during subsequent plating or enrichment (literature reports in food/environmental microbiology).
• The solution is generally not a true buffer; pH is primarily governed by the water and the weakly buffering amino groups/carboxylates present in peptone. For processes where pH control is essential, buffered variants (e.g., BPW, PBS) are typically chosen.
• Typical uses reflect these roles: diluent for serial dilution, sample transport fluid, and rinse medium to detach cells from surfaces or filters while preserving viability for downstream enumeration.

Buffer Applications

This product is not a defined buffer system; peptone–saline mixtures exhibit minimal intrinsic buffering capacity (general/literature). Nevertheless, they are widely used in buffer-like roles for microbiological workflows.

• When to use:
  • As an isotonic diluent for serial dilutions and sample rinsing where strict pH control is not critical and minimal nutrients aid recovery.
  • For short-term sample holding and transport prior to enrichment or plating.
• When to choose a true buffer instead:
  • If your method requires defined buffering capacity or specific ionic composition (e.g., PBS, phosphate-buffered peptone water, Tris-buffered saline) to stabilize pH during prolonged manipulations.
• Practical preparation hints (literature/general; not item specifications):
  • Commonly prepared at ~0.85–0.90% NaCl with ~0.1% peptone in purified water; adjust pH near neutral if required by method, then sterilize by autoclaving or membrane filtration depending on heat stability and clarity requirements.
  • For sterile applications, filter through 0.22 µm in a clean environment; verify sterility by negative controls.
• Compatibility: Generally compatible with standard plating media; avoid phosphate-sensitive assays if substituting with PBS; confirm that the presence of peptides does not interfere with downstream biochemical assays.

Green Alternatives

• Intrinsic profile: This is a water-based solution with benign inorganic salt (NaCl) and biodegradable organic content (peptone). It is already aligned with green chemistry principles (safer solvent/media, low VOCs, minimal flammability).

• Sustainability considerations (general/literature):

  • Concentrates/powders vs ready-to-use solutions: Using dehydrated media or concentrates reduces shipping weight and packaging volume; prepare on-site with purified water and sterile filtration/sterilization. Tradeoff: requires local QA controls and equipment.
  • Reuse of containers: Where validated, employ returnable or recyclable containers; ensure containers contacting biological materials are treated as biohazard after use.
  • Energy usage: Store at 2–8°C as specified; avoid unnecessary freezing or prolonged cold storage beyond shelf life to minimize energy consumption while maintaining quality.
  • Waste minimization: Prepare and aliquot only what is needed for the day’s work. Dispose of biologically contaminated liquids via approved disinfection and drain disposal rules or regulated medical waste pathways per local regulations.

• Alternatives to consider (use-case dependent):

  • Phosphate-buffered saline (PBS) when buffering is critical and nutrients are undesirable.
  • Buffered peptone water (BPW) when pre-enrichment is required. These are functional alternatives rather than greener per se; selection should follow method compliance and performance requirements.

Pharmaceutical Uses

No therapeutic or clinical claims are made. For research and laboratory use only.

General lab/manufacturing context (literature/general):

• Microbiological quality control: Used as a diluent/transport/wash solution in QC laboratories (e.g., environmental monitoring, bioburden testing, and cleaning validation recoveries) where an isotonic medium that supports organism viability during handling is beneficial.
• Sample preparation: Rinsing of equipment parts, swabs, and filters prior to microbial recovery on selective/differential agars.
• Method alignment: Selection should follow compendial or internal SOPs (e.g., USP/EP/JP or ISO methods) that specify diluent composition, sterility, and pH. Confirm the product’s attributes via CoA to ensure method suitability.
• Excipient/formulation role: Not typically used as a pharmaceutical excipient; rather, it functions as a laboratory reagent to support microbiological procedures associated with pharmaceutical production and testing.
• Regulatory note: Where sterility, endotoxin, or particulate limits are mandated by the method, use lots that meet those specific requirements and document traceability. For release or validation work, maintain consistent composition and lot control to ensure reproducibility.

Physical Properties

Item-specific specifications are not provided in the Product Data. Do not interpret the following as product specifications; they are literature/general expectations for peptone–saline diluents.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet. (Typically a clear, colorless to slightly straw-colored aqueous solution; literature.)
• Concentration/composition: Not specified for this item; refer to CoA/Spec Sheet. (Common formulations include peptone at ~0.1% w/v and NaCl at ~0.85–0.90% w/v; literature.)
• pH (literature): Often near neutral (approx. 7.0–7.4 at 25°C), depending on peptone source and water quality.
• Density (literature): ~1.00–1.01 g/mL at 20–25°C for dilute saline/peptone solutions.
• Osmolality/conductivity: Not specified for this item; refer to CoA/Spec Sheet. (Typically comparable to physiological saline; literature.)
• Boiling/Melting points: Governed by water; BP ~100°C, MP ~0°C (literature), slightly shifted by dissolved solids.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet. (Close to that of water for dilute solutions; literature.)
• Solubility: Miscible with water (peptone and NaCl are water-soluble; literature). Not intended for use with organic solvents.
• Sterility/bioburden: Not specified for this item; refer to CoA/Spec Sheet.
• Endotoxin/biochemical assays: Not specified for this item; refer to CoA/Spec Sheet.

Quality and Grades

• Grade: BioReagent (item-specific). This designation typically indicates suitability for biological and microbiological laboratory use, with raw materials and processing intended to minimize contaminants that may interfere with common bioassays or culture workflows.
• What BioReagent commonly entails (general/literature):
  • Controlled sourcing of peptone; typical checks may include clarity, pH range, low heavy metal content, and microbial limits. Exact test list and limits vary and are not specified for this item; refer to CoA/Spec Sheet.
  • For solutions, additional attributes can include filtration level/sterility, osmolality or conductivity range, and particulate/clarity criteria (not specified here).
• Stabilizers/preservatives: Not specified for this item; refer to CoA/Spec Sheet. If a preservative is present in any lot, it may influence downstream microbiological recovery; verify before use in sensitive assays.
• UV absorbance, endotoxin, bioburden: Not specified for this item; refer to CoA/Spec Sheet.
• Practical notes for users:
  • For quantitative microbiology (e.g., serial dilutions for plate counts), verify sterility and composition match your method validation (ISO, USP, EP, BAM, etc.).
  • If using for environmental monitoring or bioburden testing, match the lot’s pH/osmolarity to your SOP and record CoA values in batch records.
  • When high reproducibility is required, maintain single-lot usage for a campaign and document lot-to-lot equivalency.

Reaction and Applications

This product is not designed for synthetic organic or inorganic reactions. Instead, its applications are in microbiology and bioprocess workflows (literature/general):

• Microbiological diluent for serial dilutions prior to plate counts (e.g., total aerobic count, coliforms), supporting viability during handling by providing osmotic balance and trace nutrients.
• Sample collection and rinsing fluid for swabs, filters, or surfaces, where peptone mitigates cellular stress relative to saline alone.
• Cell wash/suspension medium for non-fastidious organisms when minimal nutrient carryover is desired.
• Transport of environmental or food samples to the laboratory prior to enrichment, when a non-buffered, isotonic medium is suitable.
• Device/flask rinse for harvesting microbes from surfaces prior to enumeration. Practical tips:
• Use aseptic technique; pre-warm to room temperature if method specifies. Vortex thoroughly to ensure homogeneous dispersal of particulates.
• For quantitative assays, validate diluent lot with control strains and ensure sterility and pH match your SOP. Document hold times and temperatures.
• Avoid using as a long-term culture medium; nutrient levels are generally insufficient for sustained growth compared with enriched media (e.g., TSB, BPW).

Reaction Conditions

Not applicable to chemical reaction optimization. For microbiological handling (general/literature guidance):

• Temperature: Typically used at ambient temperature (20–25°C). For sensitive organisms, pre-equilibrate to room temperature to avoid cold shock.
• Atmosphere: No special atmosphere required. Maintain aseptic conditions to prevent contamination.
• Contact times: Keep holding times minimal before plating/enrichment to avoid changes in microbial counts due to die-off or unintended growth; follow method-specific limits (e.g., minutes to a few hours).
• Containers: Use sterile, endotoxin-controlled vessels where required by SOPs. Mix gently but thoroughly to homogenize suspensions without shearing fragile cells.
• Additives: Avoid adding antimicrobials or detergents unless explicitly called for by your method. Any preservative would alter recovery and is not specified for this item; refer to CoA/Spec Sheet. These points are general best practices and not item specifications.

Safety and Handling

• GHS/Signal Word/Pictograms/H-Statements: Not specified for this item; refer to the SDS for authoritative hazard classification.
• General hazard profile (literature/general): Aqueous peptone–saline solutions are typically low hazard but are non-sterile or may become contaminated during use; handle as potentially biohazardous after contact with biological materials.
• Recommended PPE: Laboratory coat, gloves (nitrile), and safety glasses. Use aseptic technique when sterility is required.
• Handling notes:
  • Avoid microbial contamination; work in a biosafety cabinet when preparing or handling sterile dilutions/cultures.
  • Do not mix with incompatible disinfectants/oxidants if residual organic load (peptone) may cause reactions or neutralize sanitizer efficacy.
  • Prevent aerosol generation during pipetting or vortexing of microbiological samples.
• First aid (general):
  • Skin/eye contact: Rinse with water. Remove contaminated clothing. Seek medical advice if irritation persists.
  • Ingestion/inhalation: Rinse mouth, move to fresh air; obtain medical attention as needed.
• Spills: Absorb with paper towels/appropriate absorbent. If biologically contaminated, apply suitable disinfectant (e.g., freshly prepared 0.5–1% sodium hypochlorite) and allow appropriate contact time before disposal per institutional biosafety procedures.
• Storage incompatibilities: Avoid contamination with strong oxidizers if not intended (can degrade organics). Keep container tightly closed; maintain cold chain as specified.
• Always consult the SDS and institutional biosafety manuals for definitive guidance.

Solvent Selection

This product is an aqueous microbiological diluent/solution rather than a solvent for chemical synthesis. Classic solvent-selection criteria (e.g., polarity tuning for reactions or extractions) are not typically applicable.

• Polarity and miscibility (general): Water-based; fully miscible with water and other aqueous media. Not recommended for mixing with organic solvents for reaction chemistry.
• Use context: Choose this solution when an isotonic, nutrient-containing aqueous diluent is desired for handling microorganisms or biological samples (e.g., sample rinsing, serial dilution, resuspension) rather than pure saline or phosphate-buffered saline.
• Alternatives (literature/general comparison):
  • Normal saline (0.85–0.90% NaCl) — isotonic but lacks nutrient/peptide content; may reduce microbial recovery during stress compared with peptone-containing diluents.
  • Phosphate-buffered saline (PBS) — adds buffering capacity but no nutrients; phosphate may interfere with certain assays or downstream processes.
  • Buffered peptone water (BPW) — includes buffering and higher peptone content; chosen for pre-enrichment rather than simple dilution. Select based on your method (e.g., ISO/USP) and the required osmotic protection, buffering, and nutritional minimality.

Storage and Reconstitution

• Storage (item-specific): Store at 2–8°C. Shipped on wet ice to maintain the cold chain.
• Shelf life/expiry: Not specified for this item; refer to CoA/Spec Sheet or product label.
• Freeze–thaw: Not specified for this item; as a general precaution, avoid freezing aqueous peptone–saline unless validated, as precipitation or container stress may occur (literature/general).
• Light sensitivity: Not typically light-sensitive; store in original, closed container to prevent contamination. Specific requirements not specified for this item; refer to CoA/Spec Sheet.
• Aseptic handling:
  • Open only in clean conditions. If sterility is required after first opening, consider aliquoting into sterile containers and, if method permits, sterile-filter before use.
  • Record opening date and employ first-in/first-out practices.
• Reconstitution: Not applicable; this product is supplied as a solution. If using concentrates or powders as alternatives, prepare with purified water, adjust pH if required by your method, and sterilize by autoclaving or membrane filtration (literature/general).
• Contamination control: Discard if turbidity, precipitate, or off-odor develops, or if contamination is suspected. Always consult the CoA/Spec Sheet for lot-specific details.

Structure and Identity

• Item: Peptone Sodium Chloride Solution (SKU: P1528259)
• Nature: Aqueous mixture of undefined composition (complex digest “peptone” plus sodium chloride). Not a single, well-defined small molecule.
• CAS, CID, InChIKey, SMILES: Not applicable to complex mixtures; not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula / molecular weight: Not applicable to mixtures; not specified for this item; refer to CoA/Spec Sheet.
• Structural features (general chemistry knowledge):
  • Contains a heterogeneous distribution of peptides, free amino acids, and small proteins derived from enzymatic digestion of protein (peptone component), together with inorganic NaCl in water.
  • No fixed stereochemistry or defined ring system; composition varies by peptone source and lot within controlled specifications.
• 2D description in words (general):
  • Continuous aqueous phase with solvated sodium (Na+) and chloride (Cl−) ions providing ionic strength/osmotic balance.
  • Peptone constituents comprise short peptide chains and amino acids bearing amide, carboxylate, amine, and side-chain functional groups, collectively supplying nutritional and protective effects for microorganisms during handling and dilution steps.
• Identity confirmation (general practice):
  • Typically verified by supplier CoA parameters such as pH, clarity, sterility/microbial limits, conductivity/osmolality, and total nitrogen or peptide content (literature/general), not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

This product is not a building block, catalyst, or reagent for chemical synthesis. It is an aqueous biological reagent intended for microbiology workflows. Consequently:

• No typical roles in organic transformations, polymerizations, or inorganic syntheses.
• Not suitable as a reaction solvent due to undefined organic content (peptone) and water base.
• If a mild, isotonic aqueous environment is required for handling biological materials upstream of chemical analysis, this solution can serve as a sample matrix; however, verify that peptides/salts do not interfere with your analytical method (e.g., LC–MS ion suppression, derivatization side reactions).

Target Specificity

Not applicable. This product is not an antibody, probe, or targeted binding reagent. It is a general-purpose microbiological diluent/solution intended for broad use with diverse microorganisms.