Phosphate acetyltransferase (PTA） , CAS No.9029-91-8

CAS: 9029-91-8 Cat. No.: P1431341 Número CE: 232-855-2

GRAU & PUREZA Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥3000 U/mL; ~3500 U/mg protein

Accession #

P39646

Bioactivity

≥3000 U/mL; ~3500 U/mg protein

Visão geral

High purity recombinant phosphotransacetylase (Bacillus subtilis) for use in research, biochemical enzyme assays and analytical testing applications. 

Specificity: 

Catalyses the reaction: Coenzyme A + acetyl phosphate = acetyl-CoA + phosphate

Temperature Optima: 25°C

pH Optima: 7.4

Application examples: Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.

PROPERTIES 

1. ELECTROPHORETIC PURITY: 

- Single band on SDS-gel electrophoresis (MW ~ 36,953) 

- Single major band on isoelectric focusing (pI ~ 5.3) 

2. PHYSICOCHEMICAL PROPERTIES: Recommended conditions of use are at pH 7.4 and up to 25°C. 

3. STORAGE AND USE CONDITIONS/RECOMMENDATIONS: The enzyme is supplied as an ammonium sulphate suspension containing 0.5 mg/mL BSA and should be stored at 4°C. For assay, this enzyme should be diluted in 100 mM Tris.HCl buffer, pH 7.4 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use. 

Especificações

Product Name

Phosphate acetyltransferase (PTA） , CAS No.9029-91-8

Sinónimos

Acetyl-CoA:orthophosphate acetyltransferase | phosphate acetyltransferase | phosphoacylase | phosphotransacetylase | PTA

Grau

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Especificações e pureza

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥3000 U/mL; ~3500 U/mg protein

Bioatividade

≥3000 U/mL; ~3500 U/mg protein

Número de acesso

P39646

Peso molecular previsto

36,953

CAS

9029-91-8

Número da Comissão das Enzimas

EC 2.3.1.8

Tipo de molécula

Enzyme

Product Line

Produtos biológicos

Armazenamento e envio

Concentração

≥3000 U/mL; ~3500 U/mg protein

Condições de armazenamento de armazenamento

Store at 2-8°C

Enviado em

Wet ice

Estabilidade e armazenamento

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot.

Definição da unidade

One Unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one µmole of acetyl-CoA per minute from Coenzyme A (0.44 mM) in Tris.HCl buffer (86 mM) pH 7.4 at 25°C.