pLenti-CBLL2-sgRNA

Cat. No.: P744456

Specifications

Product Name

pLenti-CBLL2-sgRNA

Product Line

biological_products

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at -80°C

Enviado em

Dry ice packs + Cold packs

Estabilidade e armazenamento

Stored at -20 ℃, valid for at least 6 months;Stored at -80 ℃, valid for at least one year.

Application Protocols

Only product-validated protocols and conditions from the catalog/CoA may be listed. None are provided for this SKU. • Tested applications: Not specified for this item; refer to CoA/Spec Sheet. • Recommended conditions/dilutions: Not specified for this item; refer to CoA/Spec Sheet. • General note: Follow your institution’s biosafety approvals and consult the SDS and CoA for any handling instructions. This entry intentionally omits procedural steps related to gene editing or lentiviral work.

Biological Roles

• Construct role (general): Plasmid-encoded sgRNA cassettes provide sequence-specific guidance to CRISPR-associated nucleases, enabling targeted DNA binding/cleavage in research settings. • Target context: “CBLL2” refers to a human gene symbol (Cbl proto-oncogene like 2). The specific sgRNA spacer sequence and genomic target site for this SKU are not specified; refer to CoA/Spec Sheet. • Mechanistic overview (literature, high level): The sgRNA contains a variable spacer (typically ~20 nt) that base-pairs with the genomic target adjacent to a PAM appropriate for the chosen Cas enzyme, plus an invariant scaffold that complexes with the nuclease. Complex formation can mediate site-specific double-strand break formation or deaminase/reverse-transcriptase recruitment in editing variants. This is conceptual information only and not a use protocol. • Off-target considerations (general): Sequence selection and validation are critical to reduce off-target binding. Empirical validation and appropriate controls are recommended. No specific performance data are provided for this item.

Buffer Applications

Not typically applicable. This product is a plasmid DNA construct, not a buffering reagent.

• Practical note: If buffer composition is relevant to your work (e.g., TE vs. nuclease-free water), consult the item’s CoA/Spec Sheet. Do not assume compatibility with specialized biological buffers without documentation.

Green Alternatives

Not applicable. This SKU is a plasmid DNA construct rather than a solvent or small-molecule reagent where green-chemistry solvent substitution would be relevant.

General sustainability notes (non-item-specific): • Minimize single-use plastics by planning aliquots judiciously. • Use enzyme- and buffer-efficient workflows to reduce waste. • Coordinate consolidated dry ice shipments to reduce packaging footprint where feasible within stability constraints.

Pharmaceutical Uses

Not applicable. This RUO plasmid DNA construct is intended for laboratory research only and is not supplied under pharmacopeial or GMP specifications. It is not an excipient or a component of approved pharmaceutical formulations.

• If translation to regulated settings is planned, sequence, quality attributes (e.g., impurity profiles, endotoxin), and manufacturing controls would need to be established under appropriate quality systems. Such data are not provided for this SKU.

Physical Properties

• Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet. • Concentration/formulation: Not specified for this item; refer to CoA/Spec Sheet. • Buffer/salt content: Not specified for this item; refer to CoA/Spec Sheet. • pH (solution): Not specified for this item; refer to CoA/Spec Sheet. • Extinction coefficient/A260–A280: Not specified for this item; refer to CoA/Spec Sheet. • DNase/RNase status: Not specified for this item; refer to CoA/Spec Sheet. • Storage conditions (item-specific): Store at −80°C (Product Data). • Shipping (item-specific): Shipped on dry ice packs + cold packs (Product Data). • Literature/general properties for plasmid DNA (for context only):

• DNA is polyanionic, water-soluble in low-salt, nuclease-free buffers (e.g., Tris-EDTA or nuclease-free water); solubility and viscosity depend on size and concentration.
• Typical A260 of 1.0 corresponds to ~50 µg/mL for dsDNA (literature). These are general reference values only and are NOT specifications for this item.

Quality and Grades

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet. No analytical grade/hydrophobic solvent specs apply to plasmid DNA. • Identity/sequence: For plasmid products, quality is typically demonstrated by sequence verification of critical regions (insert, promoter, sgRNA scaffold) and/or restriction digest profiles; item-specific data are not provided here. • Endotoxin/host-derived impurities: Not specified for this item; refer to CoA/Spec Sheet. If relevant to your application (e.g., sensitive primary cells), request endotoxin information. • Research use only: Labeled RUO; manufactured for laboratory research workflows, not GMP. Not suitable for clinical manufacturing unless otherwise stated. • Category note: The catalog path includes “抗体 (antibody)”, but this SKU name indicates a plasmid DNA construct (pLenti). Users should rely on the item’s CoA/map for correct modality confirmation. • Documentation recommended: Obtain the full plasmid map, sequence, antibiotic resistance marker, and selection conditions from the CoA/Spec Sheet before use. Confirm lot-specific concentration and buffer.

Reaction and Applications

Classical organic reaction applications (Grignard, cross-coupling, oxidations, etc.) do not apply to a plasmid DNA construct.

High-level research context (non-procedural): • sgRNA expression plasmids are used in CRISPR workflows to provide a guide sequence and scaffold that direct a Cas nuclease to a genomic locus (here denoted “CBLL2”). • pLenti backbones are often chosen when downstream lentiviral packaging is anticipated at facilities authorized for such work. This entry does not include any procedural steps; consult your institutional biosafety program and product CoA. • Typical upstream considerations include verification of the sgRNA spacer sequence, target PAM, and off-target assessment using bioinformatic tools. These are general considerations only; no sequence is specified for this item.

Reaction Conditions

Not applicable in the context of chemical reactions. No synthetic reaction conditions (solvent, temperature, catalysts) pertain to this plasmid DNA construct.

General molecular biology note (non-procedural): Enzymatic manipulations of plasmids (e.g., restriction digestion, ligation, PCR) use standard buffer systems and temperatures defined by the respective enzymes. No item-specific conditions are provided here; consult enzyme suppliers and the item’s CoA/map.

Safety and Handling

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS. • Research use note: For research use only (Product Data). Not for diagnostic, therapeutic, or clinical use. • Biosafety considerations (general, non-procedural): Plasmids encoding sgRNA cassettes are genetic engineering tools and should be handled under an institutional biosafety program appropriate to nucleic-acid work (often BSL-1 for non-infectious DNA; higher containment may be required if producing or handling lentiviral particles). Follow your IBC/IBSC approvals and local regulations. • PPE and hygiene: Wear lab coat, gloves, and eye protection. Avoid aerosol generation. Work in a clean area to minimize nuclease contamination. Decontaminate surfaces and disposable plastics with appropriate DNA-degrading or disinfectant solutions per institutional guidance. • Incompatibilities/avoid: Avoid nucleases (DNase/RNase), microbial contamination, repeated freeze–thaw, and prolonged exposure to ambient temperatures which can degrade nucleic acids. • First-aid (general): If skin or eye contact occurs, rinse with water. If inhaled or ingested accidentally, seek medical advice and show the SDS. • Waste: Dispose of nucleic-acid-containing waste according to institutional biosafety and local regulations. Autoclave or use approved chemical disinfectants as required. • Always consult the SDS and your biosafety office for authoritative, site-specific handling guidance.

Solvent Selection

This product is a plasmid DNA construct, not an organic small-molecule reagent. Traditional solvent selection guidance (polarity, dielectric constant, UV cutoff) does not apply.

• Practical note (general): Plasmid DNA is typically maintained in nuclease-free aqueous buffers (e.g., low-salt Tris with EDTA) or nuclease-free water. Exact formulation for this SKU is not specified; refer to CoA/Spec Sheet. • Avoid: Organic solvents and strong acids/bases that can denature or hydrolyze nucleic acids. Prevent nuclease contamination by using certified nuclease-free consumables.

Storage and Reconstitution

• Storage (item-specific): Store at −80°C (Product Data). Minimize freeze–thaw by keeping working aliquots. • Shipping (item-specific): Shipped on dry ice packs + cold packs (Product Data). Inspect upon receipt and return to −80°C promptly. • Reconstitution/working solution: Not specified for this item; refer to CoA/Spec Sheet. As general context for plasmid DNA (not item-specific), nuclease-free water or low-salt Tris-based buffers are commonly used; confirm compatibility with the provided formulation. • Handling: Thaw on ice, mix gently to avoid shearing, and keep nuclease-free conditions. Avoid repeated freeze–thaw cycles. • Stability: Lot-specific stability and concentration are not provided; consult the CoA for expiry dating and any on-use storage recommendations.

Structure and Identity

• Item type: pLenti-CBLL2-sgRNA appears to be a plasmid-based lentiviral sgRNA expression construct intended for laboratory gene-editing research workflows. • Backbone/features (general, literature): pLenti backbones typically contain a lentiviral long terminal repeat (LTR) framework, a U6 or H1 promoter driving a short guide RNA (sgRNA) cassette, and antibiotic selection markers; exact map and features for this SKU are not specified. • Target context (general): “CBLL2” denotes the intended gene target of the sgRNA cassette; the precise spacer sequence, PAM context, and any cloning overhangs are not specified for this item; refer to CoA/Spec Sheet. • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet. • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet. • SMILES: Not applicable for plasmid DNA; not specified for this item. • InChIKey: Not applicable for plasmid DNA; not specified for this item. • CAS/CID: Not specified for this item; refer to CoA/Spec Sheet. • Structural description (general): Circular double-stranded DNA (dsDNA) plasmid; functional regions typically include promoter(s), sgRNA scaffold, bacterial origin of replication, and selection marker(s). The precise 2D plasmid map, restriction sites, and sequence are not provided for this SKU and should be confirmed from the product documentation.

Synthetic Utility

Classical small-molecule synthetic utility (functional group transformations, named reactions) does not apply to plasmid DNA.

High-level molecular biology utility (non-procedural): • Vector backbones can be used as templates for cloning alternative guide sequences, swapping promoters, or assembling multi-guide arrays in facilities authorized for such work. • Restriction and Gibson/ligation-based assembly strategies are commonly used in the literature for plasmid engineering; specific restriction sites, overhangs, and cloning design for this SKU are not specified. Consult the CoA/Spec Sheet for the exact map before any cloning work.

Target Specificity

Only item-specific data may be listed here. • Antigen/epitope/clone/isotype: Not applicable; this product is not an antibody. • sgRNA target sequence and locus: Not specified for this item; refer to CoA/Spec Sheet. • Species reactivity: Not specified for this item; refer to CoA/Spec Sheet.