PT-262 - ≥99% , CAS No.86811-36-1

CAS: 86811-36-1 Cat. No.: P650473 Fórmula: C₁₄H₁₃ClN₂O₂ Peso molecular: 276.72 PubChem CID: 12424014

GRADE & PURITY ≥99%

Storage

Store at 2-8°C,Protected from light,Desiccated

Shipped In

Wet ice

Visão geral

PT-262 is a potent ROCK inhibitor with an IC 50 value of around 5 μM. PT-262 induces the loss of mitochondrial membrane potential and elevates the caspase-3 activation and apoptosis . PT-262 inhibits the ERK and CDC2 phosphorylation via a p53-independent pathway. PT-262 blocks cytoskeleton function and cell migration. PT-262 has anti-cancer activity

In Vitro

PT-262 (5-40 μM; 24 h) induces cytotoxicity and proliferation inhibition in human lung cancer cells. PT-262 (2-20 μM; 4-24 h) induces caspase-3 activation, mitochondrial dysfunction and apoptosis in lung cancer cells. PT-262 (10-20 μM; 24 h) induces the accumulation of G2/M phases in both the p53-wild type and p53- lung cancer cells, and inhibits the phosphorylation of CDC2 proteins. PT-262 (0-10 μM; 24 h) represses ERK phosphorylation in lung cancer cells. PT-262 (2 μM; 24 h) induces the cytoskeleton alteration and cell elongation in lung carcinoma A549 cells. PT-262 (2-10 μM; 6 h) significantly blocks the cell migration in a concentration-dependent manner. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: A549 cells Concentration: 5-40 μM Incubation Time: 24 h Result: Reduced the cell viability via a concentration-dependent manner in A549 cells. The IC 50 value toward human normal lung fibroblast was >20 μM. Apoptosis AnalysisCell Line: A549 cells Concentration: 2-20 μM Incubation Time: 4-24 h Result: The apoptotic cells were increased after treatment at 10 μM for 8-24 h. The active forms of caspase-3 (12 and 17 kD) were induced following treatment with 2-20 μM for 24 h. Cell Cycle AnalysisCell Line: A549 and H1299 cells Concentration: 10-20 μM Incubation Time: 24 h Result: Significantly decreased the G1 fractions while increased the G2/M fractions in both A549 and H1299 cells with 10 μM for 24 h. Decreased the protein levels of cyclin B1 and phospho-CDC2 at Thr14, Tyr15, and Thr161 via a concentration-dependent manner in A549 cells. Western Blot AnalysisCell Line: A549 cells Concentration: 0-10 μM Incubation Time: 24 h Result: Significantly inhibited the phosphorylation of ERK.

Form:Solid

IC50& Target:ROCK|5 μM (IC|50|)|ERK|CDK2

Specifications

Especificações e pureza

≥99%

Mecanismos bioquímicos e fisiológicos

PT-262 is a potent ROCK inhibitor with an IC 50 value of around 5 μM. PT-262 induces the loss of mitochondrial membrane potential and elevates the caspase-3 activation and apoptosis . PT-262 inhibits the ERK and CDC2 phosphorylation via a p53-independent

Condições de armazenamento de armazenamento

Store at 2-8°C,Protected from light,Desiccated

Enviado em

Wet ice

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Tipo de ação

INHIBITOR

Pureza

≥99%

Nomes e identificadores

• SMILES isoméricas: C1CCN(CC1)C2=C(C(=O)C3=C(C2=O)C=CC=N3)Cl
• PubChem CID: 12424014
• Peso molecular: 276.72

Propriedades químicas e físicas

• Solubilidade: DMSO : 100 mg/mL (361.38 mM; Need ultrasonic)

Perguntas frequentes

What is the purity of this product?

This product is supplied at ≥99% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at 2–8 °C, protected from light, desiccated. Keep the container tightly closed with desiccant — the material is moisture-sensitive.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 86811-36-1, the molecular formula is C14H13ClN2O2, and the molecular weight is 276.72 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.