GRAU & PUREZABioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥90%(SDS-PAGE)≥20U/mg enzyme powder; ≥35U/mg protein
Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥90%(SDS-PAGE),≥20U/mg enzyme powder; ≥35U/mg protein ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.
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Armazenamento e envio
Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing Envio Ice chest + Ice pads. Consulte o COA específico do lote para obter as especificações exatas.
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Documentos de qualidade
SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.
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Comprovação documental
Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.
Visão geral
A flavoprotein. The enzyme catalyses a two-electron reduction and has a preference for short-chain acceptor quinones, such as ubiquinone, benzoquinone, juglone and duroquinone. The animal, but not the plant, form of the enzyme is inhibited by dicoumarol.
Reaction
Product description
Appearance:
Yellowish amorphous powder
Source:
Microorganism
Enzyme Commission Number:
EC 1.6.5.2
CAS Number:
9032-20-6
Storage temperature:
-20℃
Activity:
≥20U/mg enzyme powder; ≥35U/mg protein
Unit definition:
One unit will convert one micromole of NADH to NAD⁺ per min at pH 8.0 at 37℃.
Properties
Stability:
Stable at -20℃ for at least two years
Molecular weight:
24KD
Isoelectric point:
6.0
Michaelis constant:
2.6×10⁻³M (NADH)
Optimum pH:
8.0-11.0
{Fig. 1}
Optimum temperature:
40℃-65℃
{Fig. 3}
pH Stability:
7.0-10.0 (25℃, 24hr)
{Fig. 2}
Thermal stability:
< 50℃ (pH 8.0, 30min)
{Fig. 4}
Inhibitors:
Co²⁺, Cu²⁺, Fe³⁺, Mn²⁺, Ni²⁺, Zn²⁺, NEM, SDS
Effect of various chemicals:
{Table 1}
Table 1. Effect of Various Chemicals on DIA
[The enzyme dissolved in 100mM Tris-HCl buffer, pH8.0 + 0.1%BSA(5U/ml) was incubated with each chemical at 37℃ for 2hr.]
ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™
Especificações e pureza
Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥90%(SDS-PAGE),≥20U/mg enzyme powder; ≥35U/mg protein
Mecanismos bioquímicos e fisiológicos
It seems to function in response to environmental stress when various electron transfer chains are affected or when the environment is highly oxidizing. It reduces quinones to the hydroquinone state to prevent interaction of the semiquinone with O2 and pr
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