REV 5975 , CAS No.101820-46-6

CAS: 101820-46-6 Cat. No.: R1441465 Fórmula: C₂₄H₃₀N₂O₅ Peso molecular: 426.51

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Visão geral

REV 5975 is a angiotensin converting enzyme (ACE) inhibitor. REV 5975 shows a partial reduction of the angiotensin I effect.

Specifications

Condições de armazenamento de armazenamento

Store at -20°C

Enviado em

Ice chest + Ice pads

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Nomes e identificadores

• Peso molecular: 426.51

Application Protocols

No validated protocols are provided for REV 5975 in the current product data.

General guidance for small-molecule library handling:

• Reconstitution: If compatible, prepare concentrated stocks in anhydrous DMSO using analytical balance and calibrated micropipettes. Vortex and, if needed, sonicate briefly to aid dissolution. Filter through a low-protein-binding 0.2 µm filter only if the compound is known to be stable and non-adsorptive.
• Working solutions: Dilute into assay buffer to the desired final concentration, maintaining a constant vehicle percentage. Include vehicle-only controls and, where appropriate, positive/negative reference controls.
• Plate handling: Use low-binding plates/tips for hydrophobic compounds. Minimize freeze–thaw cycles by aliquoting single-use volumes.

These are generic best practices and not item-specific instructions. Always adapt to your assay and compound stability profile.

Biological Roles

Item-specific biological function, targets, or pathway annotations for REV 5975 are not provided in the product data.

• Biological role/status: Not specified for this item; refer to CoA/Spec Sheet and primary literature corresponding to the exact structure.

General research context (for small-molecule library entries):

• Such compounds may be screened in vitro for modulation of enzymatic activity, protein–protein interactions, or receptor binding, contingent on structural suitability and assay design. Any observations should be treated as preliminary until confirmed with orthogonal assays and authenticated material.
• When testing in cellular systems, evaluate cytotoxicity, solubility limits, and nonspecific aggregation (e.g., detergent-sensitivity assays) to avoid false positives. Monitor DMSO carrier effects and ensure appropriate vehicle controls.
• Avoid making physiological/clinical inferences; this product is for research use only and not for diagnostic or therapeutic applications.

For rigorous annotation, pair screening results with definitive identity confirmation (LC–MS, 1H/13C NMR), purity assessment, and stability tracking under the exact assay conditions used.

Buffer Applications

No buffer system or formulation role is specified for REV 5975. As a small-molecule library entry, it is not a buffering agent.

• If used in biochemical assays, the compound is typically delivered in a carrier (e.g., DMSO) and diluted into an assay buffer chosen for the biological target (HEPES, PBS, Tris, etc.). Buffer selection should be driven by target stability and readout method, not by this compound.
• Practical notes: Maintain constant carrier percentage across all wells; include vehicle controls. Filter or centrifuge working solutions if particulates form, and consider adding low levels of nonionic detergent to mitigate aggregation where appropriate.

No pKa-anchored buffering capacity or buffer recipes are applicable to this item.

Green Alternatives

Because the molecular structure and intended use conditions of REV 5975 are not specified, “greener” choices primarily concern solvent and handling practices rather than substitution of the compound itself.

General green chemistry guidance:

• Prefer greener solvents for dissolution and processing where feasible: water > ethanol/2-propanol > ethyl acetate > acetonitrile > DMSO/acetone > dichloromethane/toluene (selection must remain compatible with your assay and compound stability).
• Minimize DMSO stock concentration volumes consistent with assay needs to reduce waste; employ miniaturized dispensing (acoustic or low-dead-volume tips) to cut solvent consumption.
• Use sealed, low-permeation microtubes or plates to reduce evaporative losses and emissions.
• Consolidate shipments and use cold-chain packaging reuse/recycling programs when possible.

Illustrative solvent comparison (general):

• Ethanol: Renewable sources available; good for moderately polar organics; higher assay tolerance than many aprotic solvents.
• Ethyl acetate: Low toxicity profile and easy removal; limited for highly polar compounds.
• Acetonitrile: Excellent chromatographic performance; higher environmental burden—use sparingly and recover via solvent recycling when possible.

Always confirm compound solubility and stability before changing solvents.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or manufacturing application is provided for REV 5975, and this product is supplied strictly for research use only.

• Regulatory status: For research use only. Not for human or veterinary use. Not for clinical or diagnostic applications.
• Formulation context: If evaluated in preclinical research, solvent carriers (e.g., DMSO, PEG 400, aqueous buffers with co-solvent) must be selected based on the compound’s actual structure and stability; such exploration is outside the scope of the current product data.
• Documentation: If you intend to use this material as an analytical reference or in nonclinical method development, request and retain the CoA/SDS for traceability, and perform independent identity/purity confirmation.

No pharmacopeial monographs or compendial grades are indicated for this item.

Physical Properties

Item-specific physico-chemical parameters are not included in the current product data for REV 5975.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Purity/grade: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• pKa/logP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/practice):

• Small-molecule library members are commonly supplied as solids or films and are often reconstituted in anhydrous DMSO for stock solutions (e.g., 10–50 mM), then diluted into aqueous buffers containing co-solvent (0.1–1% DMSO). Verify solubility and stability experimentally.
• If structure is known, predicted properties (cLogP, TPSA, pKa) can be computed with standard cheminformatics tools (e.g., ALOGPS, ChemAxon) to guide solvent selection and storage.
• Check hygroscopicity and light sensitivity empirically (mass gain upon exposure; discoloration), and document any observations for your lot record.

Quality and Grades

Current listing does not specify a formal grade or purity for REV 5975.

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/inhibitors: Not specified for this item; refer to CoA/Spec Sheet.

Interpretation and guidance:

• Compound library items are typically provided at research grade suitable for discovery assays, analytical characterization (LC–MS, NMR), and medicinal chemistry exploration. If your application is chromatography-sensitive (e.g., cellular HTS with stringent DMSO specs, UV-trace analytics), request the CoA to review residual solvent levels, purity basis (HPLC/GC/area %), and identity confirmation (MS, NMR).
• If a stabilizer is used (e.g., acid scavenger, antioxidant), it will be disclosed on the CoA. Stabilizers may influence bioassays or analytical baselines; pre-test blanks when critical.
• Batch-to-batch documentation: We recommend archiving CoA, SDS, and any vendor analytical traces in your ELN/LIMS with the Aladdin SKU (R1441465) and lot number. Where necessary, perform incoming QC (HPLC purity check, LC–MS identity, water/volatile content by KF/GC) to align with your internal acceptance criteria.

Reaction and Applications

This product is listed within a small-molecule/compound library category and is intended primarily for research screening, profiling, and reference use rather than as a general synthetic reagent.

• Manufacturer applications (catalog): Not specified beyond inclusion in a compound library; no target or pathway indicated.
• Typical research uses (general):
  • Preparation of DMSO stock solutions for biochemical or cell-based screening (when permitted by your protocols).
  • Reference standard in analytical method development (LC–MS retention time, fragmentation pattern) once structure and ionization behavior are known.
  • Control material in assay development (e.g., plate uniformity, edge-effect assessments), after verifying inertness to assay components.

If REV 5975 is to be used in chemical reactions:

• Confirm structure and functional groups from the CoA before planning transformations.
• Perform micro-scale compatibility studies to assess stability to acids/bases, temperature, and common catalysts.
• Establish impurity profile to avoid confounding downstream analytics.

Note: Do not ascribe biological activity or mechanism to this item unless supported by your lot’s documentation or peer-reviewed literature for the exact structure.

Reaction Conditions

No reaction condition guidance can be provided for REV 5975 in the absence of structural and functional group information.

General guidance (if the compound is to be transformed):

• Solvents: Choose based on solubility and reactivity profile once known (e.g., polar aprotic for SNAr or amide couplings; alcoholic solvents for esterifications/transesterifications).
• Temperature: Begin with ambient trials, then explore 0–80°C as dictated by stability screening; use inert atmosphere if air- or moisture-sensitive moieties are suspected.
• Catalysts/reagents: Select after confirming the presence of compatible functional groups (e.g., Pd for cross-coupling when aryl halide/boronate motifs are present).
• Monitoring: LC–MS and TLC for conversion/impurities; quench conditions selected to avoid degradation.

These points are general literature practices and not item-specific recommendations.

Safety and Handling

Hazard classification details are not provided in the current product data for REV 5975.

• GHS classification: Not specified for this item; refer to SDS.
• Signal word / H-statements / pictograms: Not specified for this item; refer to SDS.

General laboratory precautions (good practice):

• Handle in a chemical fume hood wearing appropriate PPE: lab coat, safety glasses, and suitable gloves (e.g., nitrile). Avoid inhalation of dusts/aerosols and contact with skin or eyes.
• Prevent cross-contamination of screening plates or analytical instruments; use clean, dry tools and dedicated solvent-resistant containers.
• Storage incompatibilities: Until specific functional groups are known, segregate from strong oxidizers, strong acids/bases, and ignition sources. Avoid prolonged exposure to heat, moisture, and light.
• First aid (overview; defer to SDS): In case of skin/eye contact, rinse with water for ≥15 minutes; remove contaminated clothing. If inhaled, move to fresh air. If ingested, rinse mouth; do not induce vomiting; seek medical attention.
• Waste: Collect organic solutions and residues as hazardous chemical waste according to institutional and local regulations.

Always consult the item-specific Safety Data Sheet (SDS) shipped with your lot for authoritative information and emergency procedures.

Solvent Selection

No item-specific solubility data are provided for REV 5975. Selection should be guided by structure (if known) and small-scale screening.

Practical workflow:

• Start with a tiered screen: DMSO, DMF, MeOH, EtOH, acetonitrile, ethyl acetate, acetone; if lipophilic, try toluene, dichloromethane, or MTBE. For aqueous use, pre-dissolve in DMSO, then dilute into buffer while maintaining a low final DMSO fraction (typically ≤0.5–1% v/v in biochemical assays).
• If basic/acidic motifs are present (when known), consider ionization to enhance solubility: small aliquots of HCl/NaOH in aqueous-organic cosolvent can help prepare concentrated stocks; confirm chemical stability to pH.
• Avoid reactive media (strong acids/bases, oxidants, nucleophiles) until compatibility is established.

Comparison notes (general):

• DMSO vs DMF: DMSO is more biocompatible at low % in assays and less volatile; DMF may better solubilize highly polar/charged species but is less preferred in cell assays.
• MeCN vs MeOH/EtOH: MeCN offers low viscosity and UV transparency; alcohols can hydrogen-bond and may alter assay readouts. Choose based on detection mode and target assay tolerance.

Storage and Reconstitution

Storage conditions (item-specific):

• Store at −20°C (as provided in the product data).
• Shipped in: Ice chest + ice pads to maintain cold chain.

Additional handling guidance (general best practice):

• Upon receipt, allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation. Work quickly in a low-humidity environment.
• If supplied as a solid or film, consider preparing single-use aliquots immediately after first opening to avoid repeated freeze–thaw and headspace moisture ingress.
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet. Common research practice for small molecules is to dissolve in anhydrous DMSO to prepare a concentrated stock, then store aliquots at −20°C or −80°C, protected from light. Verify solubility and stability for this item empirically before adopting this approach.
• Stability/retention samples: Maintain a retained reference aliquot for periodic QC (e.g., LC–MS) if the compound will be used over extended periods.

Always follow any lot-specific instructions provided on the label, CoA, or SDS.

Structure and Identity

This listing (REV 5975; CAS 101820-46-6) is supplied as a research-use small molecule entry from our compound library. Item-specific structural identifiers are not disclosed in the product data.

• Chemical name: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• CAS: 101820-46-6 (as provided)

Structural features (general notes):

• Without a disclosed structure, functional groups, ring systems, and stereochemistry cannot be summarized for this item. Please consult the Certificate of Analysis (CoA) or technical data sheet (TDS) accompanying your lot for definitive identifiers.
• For in-house registration, we recommend recording vendor SKU (R1441465), CAS (if applicable to your lot), and any batch-specific identifiers (lot number, barcodes).

2D structure description (general guidance):

• If structure is provided with your CoA, summarize the backbone (e.g., heteroaromatic vs. aliphatic), count of heteroatoms (N/O/S/halogens), and any chiral centers. This helps anticipate solubility and potential assay liabilities (e.g., PAINS motifs).

Synthetic Utility

Specific synthetic reactivity for REV 5975 cannot be described without a disclosed structure or functional group information.

General considerations if used as a building block or intermediate:

• Confirm the presence of reactive handles (e.g., halides, boronates, carboxyls, amines) by spectroscopic data before planning cross-couplings, amidations, or substitutions.
• Assess protecting group needs and chemoselectivity if multiple nucleophilic/electrophilic sites exist.
• Establish stability to bases/acids, oxidants/reductants, and heat via micro-scale stress testing prior to scale-up.

If the compound is a final screening entity rather than a reagent, synthetic utility is typically limited; instead, focus on analog synthesis guided by SAR once the core scaffold is known from your CoA or internal characterization.

Target Specificity

No target binding data, enzyme/receptor specificity, or pathway annotation is provided for REV 5975 in the current product data.

• Target/epitope/clone/isotype: Not applicable; this is not an antibody product.
• Any use as a probe or ligand must be supported by your own characterization data or authoritative literature for the exact structure and lot.

Refer to the CoA or published studies (if available for the same CAS and structure) before assigning biological specificity.