SA-HRP

Cat. No.: np156148

GRAU & PUREZA Azide Free, High Performance, Streptavidin was conjugated with enzyme under optimum conditions, and unconjugated Streptavidin and free enzyme were removed.

Visão geral

Background

Streptavidin is a non-glycosylated protein from the bacterium Streptomyces avidinii. Streptavidin homotetramers have a particularly high, non-covalent binding affinity for biotin. When conjugated with an enzyme (eg, Horseradish Peroxidase or Alkaline Phosphatase) and coupled with a colorimetric or luminescent substrate development system, streptavidin has found widespread use along with biotinylated antibodies in a number of applications including Western blot, ELISA, ELISPOT, immunocytochemistry and immunohistochemistry.

Recommended Usage：

WB: 1:10000 -1:100000

ELISA: 1:1000-1:10000

Avoid using biotin-containing solutions as diluents and solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Especificações

Product Name

SA-HRP

Sinónimos

SA-HRP (HRP-labeled Streptavidin) | Streptavidin-Horseradish Peroxidase | SAv-HRP | SA V1 | SA V2 | streptavidin | Streptavidin V1 | Streptavidin V2

Grau

Azide Free, High Performance

Especificações e pureza

Azide Free, High Performance, Streptavidin was conjugated with enzyme under optimum conditions, and unconjugated Streptavidin and free enzyme were removed.

Espécies

Streptomyces avidinii

Sequência

MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLG STFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWT VAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVG HDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ

Comprimento da proteína

Full length protein

Conjugação

HRP

Fonte

Native

Nota

This product can replace S292620.

Tipo de molécula

Protein

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Concentração

Streptavidin was conjugated with enzyme under optimum conditions, and unconjugated Streptavidin and free enzyme were removed.

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle and protected from prolonged exposure to light. Prepare working dilution on day of use.

Imagens

WB-Streptavidin (HRP) (np156148)

WB-Streptavidin (HRP) (np156148)
Various whole cell lysates were subjected to SDS PAGE followed by western blot with Streptavidin (HRP) (np156148) at dilution of 1:10000 incubated at room temperature for 1 hour. Biotin-γ-actin antibody as primary antibody. Developed using the ECL technique. Observed band size was 42kDa. Exposure time was 10s.

ELISA-Streptavidin (HRP) (np156148)

ELISA-Streptavidin (HRP) (np156148)
ELISA results of Streptavidin (HRP) (np156148) tested against Biotin-BSA. Each well was coated in duplicate with 1 ng of Biotin-BSA.

WB-Streptavidin (HRP) (np156148)

WB-Streptavidin (HRP) (np156148)
HT-29 whole cell lysate was subjected to SDS PAGE followed by western blot with Streptavidin (HRP) (np156148) at dilution of 1:5000 incubated at room temperature for 1 hour. Biotin-γ-actin antibody as primary antibody. Developed using the ECL technique. Observed band size was 42kDa. Exposure time was 8s.

Aplicação

Aplicação

Dilution info

WB

1:10000-1:100000

ELISA

1/1000-1/10000