Sauton Agar Medium Base - BioReagent

Cat. No.: S1528731

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Visão geral

For enrichment cultivation of Mycobacterium tuberculosis and preparation of BCG vaccine

Specifications

Especificações e pureza

BioReagent

Condições de armazenamento de armazenamento

Room temperature

Enviado em

Normal

Grau

BioReagent

Product Line

biological_products

Application Protocols

Follow your institution’s biosafety practices. The exact preparation instructions for this specific item are Not specified for this item; refer to CoA/Spec Sheet. The following is an example protocol (literature; not a product specification):

Example: Preparation of Sauton Agar Plates

• Calculate the required volume; measure purified water accordingly.
• Gradually add the medium base powder to water with vigorous stirring to prevent clumps.
• Heat to near boiling until the solution is uniform and agar is fully melted.
• Adjust pH if required by your formulation (literature: typically near pH 7.0 before sterilization).
• Autoclave at 121 °C for 15 minutes.
• Cool to 50–55 °C in a water bath.
• If using heat-labile supplements (e.g., selective agents), add as sterile-filtered solutions with gentle mixing.
• Pour into sterile Petri dishes (e.g., ~20–25 mL per 90-mm plate). Allow to solidify on a level surface.
• Dry plates in a biosafety cabinet until surfaces are free of excess moisture. Store inverted in sealed bags/containers at 2–8 °C if required by your SOP; shelf-life depends on storage and supplements (not specified for this item).

For slants: Dispense into tubes before sterilization or pour at an angle post-sterilization per lab SOPs.

Biological Roles

This product does not have intrinsic biological activity; rather, it provides a physicochemical environment supporting microbial growth (research use only).

Functional roles of key components (literature/general for Sauton-type media):

• Carbon source (commonly glycerol): supplies energy and carbon skeletons for biosynthesis in organisms capable of glycerol catabolism.
• Nitrogen source (commonly L-asparagine): supports amino acid and nucleotide biosynthesis, often favoring slower, more discrete colony formation.
• Buffering ions (phosphate/citrate systems): stabilize near-neutral pH during growth; phosphate contributes to cellular phosphorus requirements.
• Divalent/trace cations (e.g., Mg2+, Fe3+ salts): essential enzyme cofactors and electron transfer components.
• Agar: inert gel matrix that provides a semi-solid surface for colony development and spatial separation.

Outcome in use (literature):

• Supports study of colony morphology, pigmentation, and surface growth kinetics of organisms adapted to these nutrient conditions, notably many mycobacterial species under laboratory conditions.

Buffer Applications

This is not a dedicated buffer system. However, Sauton-type media typically incorporate phosphate and/or citrate to moderate pH around neutrality during microbial growth (literature).

• If a defined buffer is required for biochemical assays, prepare a separate buffer (e.g., phosphate or HEPES) and, if compatible, add post-sterilization under aseptic conditions.
• pH targets, buffer capacities, and exact compositions for this specific item are Not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

As a dehydrated culture medium base, environmental considerations focus on resource use, waste generation, and energy for sterilization.

• Comparative considerations (general):
  • Dehydrated media vs. ready-to-use plates: Dehydrated media reduce shipping weight/volume and plastic waste but require local energy/water for preparation.
  • Agar is a renewable, marine-derived polysaccharide. Sustainable sourcing certifications can reduce ecological impact (supplier-dependent; check documentation).
  • Energy footprint can be minimized by efficient autoclave loads and avoiding over-sterilization.

Alternative media choices (literature; application-dependent):

• Middlebrook 7H10/7H11 agars: defined media often used for similar organisms; may offer improved clarity and specific growth characteristics, but include different chemical components and potential cost/availability tradeoffs.
• Glycerol-free variants or alternative carbon sources when glycerol metabolism biases outcomes (if applicable to your research design).

Waste minimization:

• Prepare only volumes required for immediate experiments; store plates only as long as needed to limit discard.
• Decontaminate spent media by autoclaving prior to disposal following institutional biosafety procedures.

Pharmaceutical Uses

Not intended for clinical or therapeutic applications. No medical claims are made. For research use only (per Product Data).

Contextual, non-clinical uses (general):

• May be employed in pharmaceutical R&D or quality-focused research labs for exploratory microbiology, environmental isolate maintenance, or method development involving organisms that grow on Sauton-type media (literature). Any regulated QC or compendial testing requires media that meet the specific pharmacopeial monographs and validations for that purpose.

Regulatory status and compendial listings: Not specified for this item; refer to CoA/Spec Sheet and institutional requirements.

Physical Properties

• Physical state: Dehydrated culture medium base (powdered blend). Appearance specifics: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Designed to be dispersed/solubilized in purified water with heating; exact dissolution profile Not specified for this item; refer to CoA/Spec Sheet.
• pH (prepared medium): Not specified for this item; refer to CoA/Spec Sheet. (Literature: Sauton agar is commonly adjusted near neutral pH ~7.0 before sterilization.)
• BP/MP, density, refractive index, logP: Not applicable for a complex dehydrated mixture; Not specified for this item; refer to CoA/Spec Sheet.

Practical, literature-based notes (non-specification):

• Typical preparation involves gradual heating to fully dissolve components before sterilization. Agar requires heating to near-boiling to melt and forms a firm gel upon cooling (~35–45 °C gelation window; literature for agar gels).
• Turbidity and gel strength depend on agar content and cooling history; ionic strength and sugars can modulate gel firmness (literature).

Quality and Grades

• Item-specific grade: BioReagent (per Product Data). This denotes suitability for research and laboratory applications where consistent biological performance is required.
• What BioReagent typically implies (general guidance):
  • Controlled bioburden in the dry blend, consistent nutrient composition, and performance suitability for cultivation of target organisms in research settings.
  • Not intended for clinical diagnostics or therapeutic use.
• Purity specifications, performance criteria (e.g., colony-forming performance with defined strains), moisture limits, and microbial limits: Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/antimicrobials: None implied. Any additives would be declared on the CoA/Spec Sheet; otherwise assume the base medium contains only nutrient salts, carbon/nitrogen sources, and agar.
• Batch-to-batch consistency: For complex media, performance is commonly verified via growth promotion testing with representative organisms (literature practice). Specific acceptance criteria for this item are Not specified for this item; refer to CoA/Spec Sheet.

Reaction and Applications

Chemical reaction applications are not relevant. This product is a culture medium base intended for microbiological workflows.

Research applications (literature/general):

• Cultivation and maintenance of mycobacteria and related fastidious organisms on solid media for colony isolation, phenotypic characterization, and basic microbiology studies.
• Comparative nutritional studies: evaluating carbon/nitrogen metabolism (e.g., glycerol/asparagine utilization) by environmental or laboratory strains.
• Enumeration and purity checks of cultures, morphotype assessment, and general colony-based assays in research settings.
• Antibiotic/compound screening in discovery research, using defined overlays or supplements where permitted for research use only.

Practical notes:

• The base is typically reconstituted with water, adjusted (if needed) per formulation, sterilized by autoclaving, cooled to ~50–55 °C, optionally supplemented (e.g., with sterile heat-labile additives per protocol), then poured as plates or used as deep tubes/slants (literature guidance).
• For reproducibility, control casting temperature, plate thickness, and drying time; these factors affect colony size and spreading.

Reaction Conditions

Chemical reaction conditions are not applicable. The relevant “conditions” pertain to medium preparation and sterilization (literature/general):

• Hydration and dissolution: Disperse the dehydrated base into cold/room-temperature purified water with stirring; heat to fully dissolve, ensuring agar melts.
• Sterilization: Autoclave at 121 °C for 15 minutes (gravity or liquid cycle as appropriate). Avoid excessive times that may darken/caramelize components.
• Cooling and pouring: Cool to ~50–55 °C before pouring plates or dispensing into tubes; add heat-labile supplements aseptically at this stage if used.
• Solidification: Allow plates to solidify on a level surface; dry surface in a biosafety cabinet as needed to achieve consistent moisture before use.

Exact reconstitution mass per liter, target pH, and supplement guidance for this specific item are Not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

• GHS classification, signal word, pictograms, hazard statements: Not specified for this item; refer to SDS.
• General laboratory precautions (good practice):
  • Avoid inhalation of dust from dehydrated media; handle in a well-ventilated area or use local exhaust.
  • Wear appropriate PPE: lab coat, safety glasses, and nitrile gloves.
  • When preparing media, use heat-resistant gloves; hot liquids and autoclaves present burn/scald hazards.
  • Prevent microbial contamination of work surfaces; decontaminate with appropriate disinfectants after handling cultures.
• Incompatibilities: No specific chemical incompatibilities typical for dry media; avoid contact with strong oxidizers and moisture during storage to prevent caking (general guidance).
• Sterilization: Autoclave per protocol (e.g., 121 °C, 15 min; literature). Ensure vessels are vented to prevent overpressure. Do not overfill flasks to minimize boil-over.
• First aid (overview; defer to SDS): If dust is inhaled, move to fresh air. If in eyes, rinse cautiously with water for several minutes. If on skin, wash with soap and water. Seek medical attention if irritation persists.
• Waste: Autoclave or chemically disinfect used plates/broths and follow institutional biohazard disposal procedures.

Solvent Selection

Not a solvent; this is a dehydrated culture medium base. Solubilization is typically performed in purified/deionized water.

• Solvent for preparation: Water (ASTM Type I/II or equivalent laboratory-grade). Avoid hard/contaminated water which can alter gel strength and pH.
• Miscibility: The powdered blend disperses in water; agar requires heating to dissolve fully (near-boiling temperatures) before autoclaving.
• Practical tips (literature):
  • Sprinkle powder into cold or room-temperature water with vigorous stirring to prevent clumping; then heat to dissolve.
  • For high agar concentrations, pre-wet with a small volume and gradually add the remainder of water to minimize lump formation.
  • Degassing hot medium (briefly) before pouring plates reduces bubble defects.
• Alternatives: Not applicable in a “solvent selection” sense. If your application requires different osmolarity or clarity, consider alternative media formulations (e.g., Middlebrook 7H10/7H11; literature) rather than changing the solvent.

Storage and Reconstitution

• Storage conditions (dehydrated powder): Room temperature (per Product Data). Protect from moisture and excessive heat. Keep container tightly closed.
• Shipping: Normal (per Product Data).
• Shelf life, permissible humidity content, and retest date: Not specified for this item; refer to CoA/Spec Sheet.

Reconstitution guidance (general literature; not product-specific):

• Water quality: Use purified/deionized water. Final volume and grams-per-liter dosage must follow the product label/CoA.
• Dissolution: Add powder slowly with stirring; heat until agar fully melts and solution becomes clear to slightly opalescent (depending on formulation).
• Sterilization: Autoclave at 121 °C for 15 minutes unless label indicates alternative conditions.
• Post-sterilization handling: Cool to 50–55 °C before adding sterile-filtered, heat-labile components. Pour plates or dispense for slants as required.
• Prepared media storage: If prepared plates/tubes are stored, protect from dehydration and contamination; storage temperature and duration are application/SOP-dependent and Not specified for this item; refer to CoA/Spec Sheet.

Research Use Note: For research use only.

Structure and Identity

Sauton Agar Medium Base is a dehydrated, multi-component culture medium base, not a single defined chemical compound.

• SKU: S1528731
• Product Name: Sauton Agar Medium Base
• CAS: Not applicable (mixture)
• Molecular Formula: Not applicable (mixture)
• Molecular Weight: Not applicable (mixture)
• SMILES / InChIKey: Not applicable for mixtures
• Grade/Purity: BioReagent (per Product Data)
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general knowledge, literature):

• Composed of a carbon source, nitrogen source, mineral salts, and gelling agent (agar). Typical Sauton formulations include glycerol (carbon), L-asparagine (nitrogen), phosphates/citrate (buffering), magnesium and iron salts (trace/essential ions), and agar as a polysaccharide gel matrix.
• 2D “structure” is not meaningful for a mixture; function arises from the combined nutrient and ionic environment that supports growth of fastidious organisms (notably Mycobacterium spp.; literature).

Synthetic Utility

Not applicable. Sauton Agar Medium Base is not used as a chemical reagent in synthetic organic or inorganic chemistry. It serves as a microbiological nutrient matrix. For chemical synthesis topics, refer to appropriate reagents and solvents.

If your workflow integrates chemical probes with microbial assays, consider the following operational notes (general):

• Verify probe solubility and potential interference with agar gelation (some solvents/surfactants can soften gels).
• Add small-molecule test compounds as sterile-filtered solutions to molten, cooled (~50–55 °C) medium with gentle mixing to ensure homogeneity, keeping final solvent content low (commonly ≤1–2% v/v; literature) to preserve plate integrity.

Target Specificity

Antibody/biomolecule target specificity is not applicable. For culture media, “target” can be thought of as the types of organisms supported.

• Organism suitability (literature/general): Sauton-type agar formulations are traditionally used for the cultivation of mycobacteria and related slow-growing organisms under laboratory conditions. Actual growth performance depends on strain, incubation parameters, and any supplements used.

No organism performance claims are made for this specific item. For expected growth-promotion characteristics, Not specified for this item; refer to CoA/Spec Sheet.