SiR dye 1 , CAS No.1808181-14-7

CAS: 1808181-14-7 Cat. No.: S1451912 Fórmula: C₃₁H₃₁N₃O₆Si Peso molecular: 569.68

GRAU & PUREZA BioReagent, for Fluorescence analysis, for Microscopy, ≥95%(HPLC)

Synonyms

SiR dye 1 | SiR 650 NHS ester | (2,5-dioxopyrrolidin-1-yl) 3',7'-bis(dimethylamino)-5',5'-dimethyl-1-oxospiro[2-benzofuran-3,10'-benzo[b][1]benzosiline]-5-carboxylate | 2,5-Dioxopyrrolidin-1-yl 3,7-bis(dimethylamino)-5,5-dimethyl-3'-oxo-3'H,5H-spiro[diben

Storage

Protected from light,Store at -20°C,Desiccated

Shipped In

Ice chest + Ice pads

Application

Fluorescence Analysis, Protein/Antibody Labeling

Visão geral

SiR-NHS ester (also known as SiR dye 1) is a far-red fluorescent dye designed for efficient covalent labeling of biomolecules containing primary amines, such as proteins, peptides, and antibodies. The NHS ester group readily reacts with ε-amino groups of lysine residues or N-terminal amino groups under mild aqueous conditions, forming stable amide bonds.

SiR-NHS ester belongs to the family of silicon-rhodamine (SiR) dyes and combines long-wavelength fluorescence with high molar absorptivity, excellent photostability, and minimal photo- and cytotoxicity, making it indispensable for super-resolution microscopy (STED, SIM, PALM/STORM) and long-term live cell imaging. Unlike many cyanine dyes, SiR-NHS ester is stable under alkaline conditions.

The far-red fluorescence of the dye does not overlap with cellular autofluorescence, which is important for fluorescence microscopy, flow cytometry, and other bioanalytical methods.

Spectral properties

Excitation maximum, nm: 652 nm

ε, L⋅mol⁻¹⋅cm⁻¹: 1.0·10⁵ mol⁻¹·cm⁻¹

Emission maximum, nm: 674 nm

CF260: 0.116

CF280: 0.147

Especificações

Sinónimos

SiR dye 1 | SiR 650 NHS ester | (2,5-dioxopyrrolidin-1-yl) 3',7'-bis(dimethylamino)-5',5'-dimethyl-1-oxospiro[2-benzofuran-3,10'-benzo[b][1]benzosiline]-5-carboxylate | 2,5-Dioxopyrrolidin-1-yl 3,7-bis(dimethylamino)-5,5-dimethyl-3'-oxo-3'H,5H-spiro[diben

Especificações e pureza

BioReagent, for Fluorescence analysis, for Microscopy, ≥95%(HPLC)

Estabilidade e armazenamento

Store at -20℃ long term (36 months). Store in the dark. Desiccated.

Condições de armazenamento de armazenamento

Protected from light,Store at -20°C,Desiccated

Enviado em

Ice chest + Ice pads

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Grau

BioReagent, for Fluorescence analysis, for Microscopy

Pureza

≥95%(HPLC)

Product Line

S1451912-100μg

Nomes e identificadores

Peso molecular

569.68

Propriedades químicas e físicas

• Solubilidade: Soluble in ethyl acetate, methanol, acetonitrile, DMF, DCM and DMSO.

Perguntas frequentes

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1808181-14-7, the molecular formula is C31H31N3O6Si, and the molecular weight is 569.68 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.