Stresscopina (humano) - ≥95% , CAS No.352020-03-2

CAS: 352020-03-2 Cat. No.: S276274 Fórmula: C195H326N56O53S2 Peso molecular: 4367.24

GRAU & PUREZA ≥95%

Storage

Armazenar a 2-8°C

Shipped In

Gelo húmido

Visão geral

Armazenar a +4°C. O produto pode ser armazenado até 12 meses.

Especificações

Especificações e pureza

≥95%

Condições de armazenamento de armazenamento

Armazenar a 2-8°C

Enviado em

Gelo húmido

Pureza

≥95%

Nomes e identificadores

Peso molecular

4367.24

Propriedades químicas e físicas

• Solubilidade: Soluble in water

Application Protocols

Only product-data-derived information may be shown here.

• Tested applications (e.g., WB, IHC, IF, FC, receptor assays), recommended dilutions/concentrations, and positive controls: Not specified for this item in the provided Product Data.

Please consult the product CoA/Spec Sheet for any validated application notes or contact Technical Support for guidance in setting up assays with this peptide.

Biological Roles

Overview (literature/general context; not item specifications)

• Stresscopin is a commonly used name for a human CRF-family neuropeptide described in the literature as an endogenous ligand within the corticotropin-releasing factor signaling axis. Peptides in this family participate in stress-responsive neuroendocrine regulation and can modulate signaling through specific CRF receptor subtypes in vitro.

Biochemical features (general)

• Mode of action: Functions as a peptide ligand for class B GPCRs of the CRF receptor family. In cellular systems, engagement can lead to Gs-mediated cAMP production and downstream kinase pathway modulation under defined assay conditions.
• Systems context: Investigated in neuronal and peripheral cell models to map receptor selectivity, trafficking, and signaling bias relative to other CRF-family ligands.
• Structure–function: Side-chain patterns along the peptide backbone contribute to receptor recognition; sequence-specific determinants of potency/selectivity are typically explored with alanine-scan or truncation analogs (literature practice).

Use in research

• Serves as a tool to dissect receptor subtype pharmacology, benchmark assay performance, and calibrate detection windows for ligand screening campaigns.

Important

• The above summarizes general biological roles reported in the literature and is not specific to the supplied lot. No medical, diagnostic, or therapeutic use is claimed or implied.

Buffer Applications

Not typically applicable

• Stresscopin (human) is a peptide ligand and not a buffering agent. It is not used to maintain pH like classical buffers (e.g., phosphate, HEPES, Tris).

Practical notes for handling in buffers (general guidance)

• Working matrices: Use physiological buffers such as PBS, HEPES-buffered saline, or assay-specific media at near-neutral pH to maintain peptide integrity and receptor compatibility.
• Additives: A small amount of carrier protein (e.g., 0.1% BSA) can reduce adsorption losses to plastics and glass in some assays; verify compatibility with your readout.
• Filtration: If sterility is required, pass solutions through a 0.22 µm low-protein-binding filter.

Item-specific details

• Any prescribed buffer composition, concentration limits, or excipients for this SKU are not specified here; refer to the CoA/Spec Sheet for lot-specific directions.

Green Alternatives

Context

• Stresscopin (human) is a peptide reagent typically used in aqueous buffers. Green chemistry considerations mainly involve minimizing organic co-solvents, waste, and energy usage during preparation and assays.

Greener practice recommendations (general)

• Prefer water-based buffers over organic-rich solvents; avoid unnecessary DMSO.
• Prepare concentrated stocks to reduce packaging and shipping frequency; aliquot to extend shelf life and minimize waste.
• Use room-temperature dissolution when feasible; avoid excessive heating or prolonged sonication that can degrade peptides.
• Employ low-impact sanitation/sterilization (0.22 µm filtration) rather than harsh chemical sterilants when compatible.
• Opt for phosphate or HEPES buffers prepared from high-purity salts; avoid volatile organic buffers.

Balanced comparison (general)

• Aqueous buffers vs. DMSO-heavy vehicles:
  • Environmental impact: Water-based systems have lower VOC emissions and improved worker safety.
  • Performance: Peptides generally maintain native conformation and activity in near-physiological ionic strength; high DMSO can denature or aggregate some sequences.
  • Tradeoffs: Some hydrophobic peptides may require small amounts of co-solvent; validate minimal effective levels to balance solubility with green principles.

Note

• These are general guidelines. Follow any solvent or reconstitution instructions provided on the product CoA/Spec Sheet for this specific SKU.

Pharmaceutical Uses

Scope of this listing

• For research use only. Not for human or veterinary use, not for diagnostic procedures, and not for use as a drug substance or excipient.

Context (general/literature; not a claim for this item)

• Peptide standards like Stresscopin are sometimes used in pharmaceutical research laboratories as reference materials in in vitro assays to characterize receptor systems, support SAR studies, and calibrate bioassays.

Manufacturing/formulation relevance

• No pharmacopeial monograph or excipient status is indicated for this item. There are no item-specific claims regarding cGMP manufacture, residual solvents, or endotoxin limits; consult the CoA/Spec Sheet if such data are provided for a specific lot.

Good practice

• If used to support regulated studies (e.g., method development), document chain-of-custody, lot analytics (purity, identity), and storage history. For any GLP/GMP context, only use materials with appropriate quality documentation; this research-grade listing does not assert such qualifications.

Physical Properties

Item-specific physical data

• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• BP/MP, density, refractive index: Not applicable/Not specified for this peptide; refer to CoA/Spec Sheet and SDS.

General/literature characteristics for peptides of this class (guidance; not item specifications)

• Physical state: Typically obtained as lyophilized solid or solution; readily handled in aqueous buffers at neutral to slightly acidic pH. Actual state for this item is not specified above.
• Solubility: Peptides generally dissolve in water or buffered saline. Initial dissolution can be aided by brief exposure to dilute acid (e.g., 0.1% TFA or acetic acid) or basic buffer depending on sequence net charge; then adjust to working buffer. For this specific item, follow the CoA if a preferred solvent is indicated.
• pH considerations: The ionization and isoelectric behavior depend on sequence composition; without item sequence, precise pI/pKa values cannot be stated.
• Stability: Aqueous peptide solutions are generally more stable at 2–8°C for short-term and at −20°C or below for long-term, especially when aliquoted and protected from repeated freeze–thaw. For this product, only 2–8°C storage is specified; see the Storage tab and SDS/CoA for definitive guidance.

Important note

• Do not treat the above as specifications. For quantitative analytics (purity, content, counter-ions, residual solvents, water), consult the CoA/Spec Sheet for the supplied lot.

Quality and Grades

Item-specific quality

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet for purity, sequence verification, and analytical methods (e.g., HPLC, MS).
• Stabilizers/Excipients: Not specified for this item; refer to CoA/Spec Sheet.

How to interpret typical peptide quality information (general guidance)

• Peptide purity (analytical HPLC): Reported as area% under a defined gradient/UV wavelength. Research-grade peptides often range from ~90% to >98% by HPLC; confirm the exact value for this SKU on the CoA.
• Identity confirmation: Commonly established by high-resolution MS (monoisotopic mass match), sometimes complemented by amino acid analysis or sequencing. CoA should state the technique used.
• Counter-ions and salt form: TFA, acetate, or chloride are common; these influence mass, solubility, and assay background. CoA typically lists the counter-ion.
• Water and residual solvents: These can vary with lyophilization history; consult the CoA for Karl Fischer water and residual solvent data if reported.
• Endotoxin/bioburden: Only reported when relevant to cell-based workflows; verify on the CoA if your application is sensitive.

Practical note

• For quantitative biology, align assay concentrations to peptide content (correcting for salt form, residual water), not just vial mass. The CoA enables precise molarity calculations when molecular weight and content are provided.

Reaction and Applications

Reactivity

• As a peptide, Stresscopin (human) is not used as a chemical reagent in synthetic transformations; instead, it serves as a bioactive ligand in receptor pharmacology and signaling studies.

Research applications (general/literature context; not item specifications)

• Receptor pharmacology: Employed as a reference agonist in GPCR studies of the CRF receptor system. Typical uses include measuring cAMP accumulation, β-arrestin recruitment, or calcium flux in engineered cell lines expressing CRF-family receptors.
• Binding assays: Utilized in competitive or saturation binding to determine affinity and selectivity in membrane preparations or whole cells.
• Signal transduction mapping: Helps delineate downstream pathways (PKA/ERK, etc.) in cell-based assays under defined conditions.
• Systems biology: Applied to explore stress-axis signaling, neuroendocrine cross-talk, and peptide–receptor network models in vitro.

Practical considerations (general)

• Stock preparation: Prepare concentrated aqueous stocks (sterile, buffered), aliquot to avoid freeze–thaw, and validate concentration by UV peptide bond absorbance if sequence permits, or by amino acid analysis.
• Assay controls: Include vehicle controls, non-specific binding controls, and a secondary ligand to benchmark potency/efficacy across experiments.

Note

• No medical or in vivo claims are implied. Use is restricted to research applications in controlled laboratory settings. Consult the CoA for any lot-specific handling or purity notes.

Reaction Conditions

Chemical reaction conditions

• Not applicable. Stresscopin (human) is not used as a reagent in named organic reactions.

Assay conditions (general/literature guidance; not item specifications)

• Receptor assays: Often conducted in buffered saline (e.g., HBSS or PBS) with physiological Ca2+/Mg2+ at 25–37°C. Endpoint readouts may include cAMP accumulation (homogeneous TR-FRET/HTRF), β-arrestin recruitment (enzyme complementation or BRET), or ERK phosphorylation (immunoassay).
• Concentration ranges: Peptide ligands are commonly tested across ~10−12 to 10−6 M to define potency and efficacy. Exact working concentrations should be empirically determined for your cell line and detection platform.
• Incubation times: From minutes (acute signaling) to 30–60 min (steady-state cAMP/β-arrestin) depending on the assay.
• Controls: Include vehicle controls, a known comparator ligand, and where applicable receptor antagonists to define specificity.

Operational tips

• Prepare fresh dilutions in assay buffer immediately before use to minimize adsorption and degradation.
• Use low-protein-binding plastics; include 0.1% BSA if compatible with your assay to reduce nonspecific losses.

Note

• The above are general literature practices. For this SKU, no item-specific assay protocol is provided; consult your internal SOPs and validate conditions experimentally.

Safety and Handling

GHS and hazard information (item-specific)

• Signal word: Not specified for this item; refer to SDS.
• H-statements: Not specified for this item; refer to SDS.
• GHS classification/pictograms: Not specified for this item; refer to SDS.

General laboratory handling for research peptides (guidance; not item-specific hazard classification)

• PPE: Wear lab coat, safety glasses, and appropriate gloves. Handle powder in a fume hood or ventilated enclosure to avoid aerosolization.
• Avoid: Inhalation of dust/aerosols, contact with skin/eyes, and accidental ingestion. Use microbalance practices to minimize airborne particulate when weighing lyophilizates.
• First aid (general): If inhaled, move to fresh air. In case of skin or eye contact, rinse with water for several minutes; remove contaminated clothing. If ingested, rinse mouth and seek medical attention. Always follow your institutional EHS procedures.
• Incompatibilities: Strong oxidizers and extreme pH can degrade peptides. Metal-catalyzed oxidation and light can impact sensitive residues; protect from light when feasible.
• Sterility/bioburden: If sterility is critical (e.g., for cell-based assays), reconstitute with sterile buffers through a 0.22 µm filter where compatible with peptide stability.

Authoritative source

• Always consult the product SDS for definitive hazard classification, safe handling, spill response, disposal, and transport information.

Solvent Selection

Applicability

• As a research peptide, Stresscopin (human) is typically handled in aqueous media rather than organic solvent systems.

General solvent guidance for peptides (literature-based; not item specifications)

• Primary choice: Ultrapure water, PBS, or other physiological buffers at pH 6.5–7.4.
• Difficult solubility cases: Pre-wet the peptide with a minimal volume of an appropriate co-solvent (e.g., sterile 0.1% acetic acid or 0.1% TFA for basic peptides; a small amount of basic buffer for acidic peptides), then dilute with buffer. Avoid high DMSO unless required by assay constraints; peptides often perform best in aqueous systems.
• Filtration: 0.22 µm sterile filtration can help remove particulates and ensure sterility for cell-based work, provided the peptide is not adsorbed by the membrane; low-protein-binding filters are preferred.

Choosing among alternatives (context)

• Compared with hydrophobic small molecules requiring DMSO, peptides like Stresscopin generally achieve better assay performance and stability in salt-buffered, near-neutral aqueous solutions.

Important

• Because the specific sequence and salt form for this item are not disclosed here, verify any solvent recommendations on the product CoA/Spec Sheet and pilot small-scale dissolutions before preparing working stocks.

Storage and Reconstitution

Item-specific storage and shipping

• Storage conditions: Store at 2–8°C (from Product Data).
• Shipped in: Wet ice (from Product Data).

Reconstitution and aliquoting

• Reconstitution protocol, recommended solvents/buffers, and suggested aliquoting strategy: Not specified for this item; refer to CoA/Spec Sheet.

General cautions (supplemental, not item specifications)

• To maximize integrity after reconstitution, laboratories often aliquot peptide solutions and avoid repeated freeze–thaw cycles. Protect from microbial contamination by using sterile technique. However, follow only the CoA/Spec Sheet and SDS for authoritative, lot-specific instructions.

Structure and Identity

Stresscopin (human) is a bioactive peptide belonging to the corticotropin-releasing factor (CRF) peptide family, supplied for research use in receptor pharmacology and peptide biology.

Item-specific facts (from Product Data)

• Name: Stresscopin (human)
• CAS: 352020-03-2
• SKU: S276274
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general/literature context; not item specifications)

• Class: Endogenous neuropeptide of the CRF/urocortin family (commonly referred to as “Stresscopin” in the literature).
• Architecture: Linear polypeptide chain comprised of L-amino acids; lacks small-molecule functional groups and behaves as a typical peptide ligand in aqueous systems.
• Functional role: Serves as a selective ligand within the CRF receptor system in human biology (literature context); exact sequence or post-translational details are not provided for this catalog item.

2D/functional description in words (general)

• A linear peptide with side chains presenting hydrogen-bond donors/acceptors and ionic groups typical of neuropeptides. The molecular recognition surface enables high-affinity, selective engagement of CRF-family receptors in biochemical assays. Precise residue-level features for this lot are not disclosed here; consult the CoA for any sequence/residue analytics if provided.

Synthetic Utility

Not a synthetic building block

• Stresscopin (human) is a bioactive peptide ligand and is not typically employed as a reagent or intermediate in organic synthesis.

Research utility (general)

• Serves as a biological tool compound in receptor pharmacology, enabling assay development, calibration of response windows, and benchmarking of novel ligands against a known peptide standard.
• Useful in structure–activity relationship (SAR) projects as a reference for assessing potency, efficacy, and receptor selectivity in vitro.

If chemical modification is pursued (advanced, general)

• Derivatization strategies in research may include N-terminal labeling (e.g., fluorophores, biotin) or backbone/stapling analog design to probe binding, but such modifications change physicochemical properties and are outside the scope of this catalog item.

Conclusion

• For chemical synthesis objectives, consider small-molecule CRF receptor ligands or protected amino acid/peptide fragments instead; Stresscopin itself is best leveraged as a biological standard in assays.

Target Specificity

Only product-data-derived information may be shown here.

• Target/epitope, receptor subtype selectivity, species reactivity, and any clone/isotype details: Not specified for this item in the provided Product Data. Refer to the CoA/Spec Sheet or contact Technical Support for any lot-specific characterization that may be available.

Perguntas frequentes

What is the purity of this product?

This product is supplied at ≥95% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 352020-03-2, the molecular formula is C195H326N56O53S2, and the molecular weight is 4367.24 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.