Ready-to-use (RTU) for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Protected from light Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Product Characteristics
The Synthetic Blocking Buffer, Blotting is a readyto-use solution for blocking of the remaining free binding sites after coating or transfer of biomolecules to a membrane. The buffer blocks all potential binding sites of nonspecific interaction and reduces the background signal.
The Synthetic Blocking Buffer, Blotting eliminates the risk of false positive reactions in the assay, while avoiding the current safety hazards related to the use of bovine biological material, for instance BSA
Composition & Properties
The Synthetic Blocking Buffer, Blotting is a readyto-use buffer. The buffer contains no proteins, and cross reactivity is therefore avoided.
Working Procedure
1.Follow the normal procedure for transfer of the biomolecules to the membrane.
2.Dry the membrane.
3.Block the membrane by incubation in the Synthetic Blocking Buffer, Blotting for 5-10 minutes at room temperature and gentle shaking.
4.Wash the membrane in a standard washing buffer for blotting.
5. Continue the processing as usual.
Tips & Tricks
• In ELISA, the Synthetic Blocking Buffer, ELISA (cat. no. S494401) is recommended.
Handling & Storage
• Store solution at 2-8 °C.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Data | Item |
|---|---|---|---|
 E2628172 | Certificate of Analysis | Jun 04, 2026 | S494457 |
| F2306606 | Certificate of Analysis | Apr 27, 2023 | S494457 |
| F2306674 | Certificate of Analysis | Apr 27, 2023 | S494457 |
| F2306870 | Certificate of Analysis | Apr 27, 2023 | S494457 |
| F2307009 | Certificate of Analysis | Apr 27, 2023 | S494457 |
| Sensibilidade | Light sensitive |
|---|
