TNT Buffer (10×) - sterile-filtered, BioReagent, Suitable for molecular biology, 10×

Cat. No.: T476217
Disponível para encomenda
GRADE & PURITY Sterile-filtered ? Sterile-filtered — passed through a 0.2µm filter to remove microorganisms. Use as a ready aseptic solution for culture and sensitive applications. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. 10×
Synonyms
TRIS NaCl Tween 20 buffer solution | Tris-NaCl-Tween buffer | 10X TNT Buffer
Storage
Room temperature
Shipped In
Normal
Application
Enzyme activity assay, Protein purification
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Size
Alemanha (EU)
USA*
Price
Qty
500ml
T476217-500ml
Sob encomenda · 8–12 semanas
17,27€
Enter a quantity for the sizes you want to add.
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Why this grade

sterile-filtered, BioReagent, Suitable for molecular biology, 10× BioReagent,Sterile-filtered,Suitable for molecular biology for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Room temperature Ships Normal Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Visão geral

  Tris-NaCl-Tween Buffer, abbreviated as TNT Buffer, is a commonly used buffer in molecular biology and hybridization experiments. It mainly consists of 100 mM Tris-HCl (pH 8.0), 1.5 M NaCl, and 0.5% Tween. The product is prepared through strict sterilization by filtration with a 0.45 μm pore size membrane. This product is a 10× concentrated solution; after filtration sterilization, it only needs to be diluted to the working concentration with sterile distilled water or deionized water before use.
Operating Steps:
1. Dilute to 1× with distilled water or deionized water.
2. Use the diluted buffer for washing as required by the experiment.
Precautions:
1. After opening the reagent, use it as soon as possible to avoid affecting the results of subsequent experiments.
2. This product is limited to scientific research use by professional personnel. It shall not be used for clinical diagnosis or treatment, nor for food or pharmaceuticals, and shall not be stored in ordinary residential premises.
3. This product does not contain highly toxic substances; however, for your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

Sinónimos
TRIS NaCl Tween 20 buffer solution | Tris-NaCl-Tween buffer | 10X TNT Buffer
Especificações e pureza
sterile-filtered, BioReagent, Suitable for molecular biology, 10×
Estabilidade e armazenamento
Store at room temperature long term (12 months).
Informações legais
TWEEN is a registered trademark of Croda International PLC
Condições de armazenamento de armazenamento
Room temperature
Enviado em
Normal
Grau
BioReagent, Sterile-filtered, Suitable for molecular biology

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificados(CoA,COO,BSE/TSE e Mapa de Análise)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

1 results found

Lot NumberCertificate TypeDataItem
ZJ25F1028459Certificate of AnalysisAug 03, 2026 T476217
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