ULK1-IN-2 - ≥99% , CAS No.2497409-01-3

CAS: 2497409-01-3 Cat. No.: U649922 Fórmula: C₁₉H₁₆BrFN₄O₆ Peso molecular: 495.26

GRADE & PURITY ≥99%

Storage

Store at 2-8°C,Protected from light,Desiccated

Shipped In

Wet ice

Visão geral

ULK1-IN-2 (compound 3s) is a potent ULK1 inhibitor. ULK1-IN-2 shows highest cytotoxic effect against cancer cell lines, with IC 50 of 1.94 μM in A549. ULK1-IN-2 can induce apoptosis and simultaneously block autophagy , and can be used to study NSCLC (Non-small cell lung cancer)

In Vitro

ULK1-IN-2 (compound 3s) (10 μM, 24 h) shows strong anti-proliferative activity against A549, U937, HL60, MDA-MB-468 and MCF-7. ULK1-IN-2 (0-8 μM, 24 h) blocks autophagy via inhibiting ULK1 in A549 cells. ULK1-IN-2 (0-8 μM, 24 h) induces apoptosis via the mitochondrial pathways in A549 cells in dose department manner. ULK1-IN-2 (0-8 μM, 24 h) inhibits ULK1 and p-ULK1 ser317 expression in a concentration-dependent manner, remarkably decreases Bcl-2 expression, increases Bax and the active form of Caspase-3 expression.. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Proliferation Assay Cell Line: Human cancer cell lines A549, U937, HL60, MDA-MB-468 and MCF-7Concentration: 10 μM Incubation Time: 24 h Result: Significantly improved anti-proliferative activity against A549, U937, HL60, MDA-MB-468 and MCF-7, with kinase inhibitory activity of 99.15% and IC 50 values of 1.94, 12.92, 10.89, 16.83, and 19.60 μM, respectively. Cell Autophagy Assay Cell Line: A549 cellsConcentration: 0, 2, 4, 8 μM Incubation Time: 24 h Result: Blocked autophagy of A549 cells via inhibiting ULK. Western Blot Analysis Cell Line: A549 cellsConcentration: 0, 2, 4, 8 μM Incubation Time: 24 h Result: Inhibited expression of ULK1 and p-ULK1 ser317 in a concentration-dependent manner. Increased the autophagy substrate P62, reduced LC3-I conversion to LC3-II, and decreased the levels of Beclin1. Remarkably decreased Bcl-2 expression, increased Bax and the active form of Caspase-3 expression.

Form:Solid

IC50& Target:ULK1

Specifications

Especificações e pureza

≥99%

Mecanismos bioquímicos e fisiológicos

ULK1-IN-2 (compound 3s) is a potent ULK1 inhibitor. ULK1-IN-2 shows highest cytotoxic effect against cancer cell lines, with IC 50 of 1.94 μM in A549. ULK1-IN-2 can induce apoptosis and simultaneously block autophagy , and can be used to study NSCLC (Non-

Condições de armazenamento de armazenamento

Store at 2-8°C,Protected from light,Desiccated

Enviado em

Wet ice

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Pureza

≥99%

Nomes e identificadores

• Sorrisos canónicos: COC1=C(OC)C(OC)=CC(NC2=NC=C(Br)C(OC3=CC=C([N+]([O-])=O)C=C3F)=N2)=C1
• Peso molecular: 495.26

Propriedades químicas e físicas

• Solubilidade: DMSO : 33.33 mg/mL (67.30 mM; Need ultrasonic)

Perguntas frequentes

What is the purity of this product?

This product is supplied at ≥99% purity (chemical assay). Lot-specific values are stated on the Certificate of Analysis.

How should this product be stored?

Store at 2–8 °C, protected from light, desiccated. Keep the container tightly closed with desiccant — the material is moisture-sensitive.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2497409-01-3, the molecular formula is C19H16BrFN4O6, and the molecular weight is 495.26 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.