Uricase (UAO), CAS No.9002-12-4

CAS: 9002-12-4 Cat. No.: R1506897 Número CE: 232-655-5 PubChem CID: 14228836

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥80%(SDS-PAGE), expressed in E.coli

Accession #

G8ZWT4

Expression system

E. coli

Bioactivity

8-12 U/mg enzyme powder

Visão geral

This product is a recombinantly expressed urate oxidase (UAO) intended for in vitro research use.

Assay Procedure

Principle: 

The disappearance of uric acid is measured at 290nm by spectrophotometry.

Unit definition:

One unit will oxidase one micromole of uric acid per minute at pH8.5 at 25℃

Reagents:

A. Uric acid solution:0.001% [Dilute the stock solution (0.01%) to 10-fold volume with 50mM borate buffer containing 0.001% Triton X-100 and 1.0mM EDTA, pH8.0] (should be prepared fresh)

Stock solution:10.0mg uric acid/100ml of above buffer (Store at 0~5℃)

B. KOH solution: 20%

C. Enzyme diluent: 50mM borate buffer containing 0.001% Triton X-100 and 1.0mM EDTA, pH8.0.

Procedure:

1. Prepare the following reaction mixture in a test tube and equilibrate at 37℃ for about 5 minutes.2.0ml Uric acid solution (Reagent A) 0.5ml Distilled water
2. Add 0.5ml of the enzyme solution* and mix by gentle inversion.
3. After exactly 5 minutes at 37℃, add 0.2ml of 20% KOH solution (B) to stop the reaction and measure the optical density at 290nm against water (OD test).At the same time, prepare the blank by first mixing the reaction mixture with 0.2ml KOH solution after 5min-incubation at 37℃, followed by the addition of the enzyme solution (OD Blank).

*:Dissolve the enzyme preparation in ice-cold enzyme diluent (C) and dilute to 0.01~0.02U/ml with the same buffer and store on ice.

Calculation:

Activity can be calculated by using the following formula:

Vt: Total volume (3.2ml)

Vs: Sample volume (0.5ml)

12.2: Millimolar extinction coefficient of uric acid (cm²/μmol)

t: Reaction time (5 minutes)

1.0: Light path length (cm)

df: Dilution factor

C: Enzyme concentration in dissolution (c mg/ml)

Specifications

Product Name

Uricase (UAO), CAS No.9002-12-4

Sinónimos

Urate: oxygen oxoreductase | factor independent urate hydroxylase | urate oxidase | uric acid oxidase | uricase | uricase II

Grau

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Especificações e pureza

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥80%(SDS-PAGE),expressed in E.coli

Bioatividade

8-12 U/mg enzyme powder

Número de acesso

G8ZWT4

CAS

9002-12-4

Número da Comissão das Enzimas

EC 1.7.3.3

Tipo de molécula

Enzyme

Armazenamento e envio

Concentração

expressed in E.coli

Reconstituição

Reconstitute in ice-cold 50mM borate buffer containing 0.001% Triton X-100 and 1.0mM EDTA, pH8.0.

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at -20℃ long term (12 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Definição da unidade

One unit will oxidase one micromole of uric acid per minute at pH8.5 at 25°C.

Perguntas frequentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥80% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Bioactive mean?

Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 9002-12-4.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.