X-34 (Amyloid-Specific Fluorescent Dye) - BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥90%(HPLC) , CAS No.215294-98-7

CAS: 215294-98-7 Cat. No.: X1444884 Fórmula: C₂₄H₁₈O₆ Peso molecular: 402.4 PubChem CID: 9930943

GRADE & PURITY BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥90%(HPLC)

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Staining‌, Fluorescence Analysis

Visão geral

X-34 is a lipophilic and highly fluorescent marker used to examine pathological changes in Alzheimer’s disease (AD). This cellular dye was developed as a derivative of Congo red to be more compatible with in vivo applications. By replacing the azo groups (nitrogen-to-nitrogen double bonds) in Congo red with carbon-to-carbon double bonds and having more lipophilic salicylic acid groups, X-34 has proven to be highly fluorescent and have increased membrane permeability. X-34 detects β-sheet structures and labels amyloid plaques, neurofibrillary tangles (NFTs), neuropil threads, and vascular amyloid in AD brains (1).

Product Usage Information

Reagents:

1. X-34:Create a 5 mM stock solution of X-34 (200X) using the X-34 diluent. Create a 25 µM staining solution by further diluting the 5 mM stock solution using the X-34 diluent.

Note: X-34 diluent (40% EtOH, pH 10) is the recommended solvent for staining. A slight precipitate may be present, but will not interfere with the product's performance.

2. X-34 diluent (40% EtOH, pH 10): Combine 20 mL of EtOH in 29.5 mL of dH2O. Add 1 µL of 5M NaOH dropwise until the solution reaches pH 10. Bring the final volume of X-34 diluent to 50 mL with dH2O.

3. Differentiation buffer: 50 mM NaOH, 80% EtOH

Protocol:

1. Stain slides in 25 µM X-34 staining solution for 30 min at room temperature in the dark.

2. Rinse slides three times in dH2O.

3. Incubate for 2 min in the differentiation buffer.

4. Rinse slides three times in dH2O.

5. If desired, proceed with immunostaining.

6. Mount and image.

Specifications

Especificações e pureza

BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥90%(HPLC)

Estabilidade e armazenamento

Store at -20℃ long term (12 months).

Condições de armazenamento de armazenamento

Store at -20°C

Enviado em

Ice chest + Ice pads

Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.

Grau

Biological Stain, BioReagent, for Fluorescence analysis, for Microscopy

Pureza

≥90%(HPLC)

Nomes e identificadores

• Sorrisos canónicos: C1=CC(=CC=C1C=CC2=CC(=C(C=C2)O)C(=O)O)C=CC3=CC(=C(C=C3)O)C(=O)O
• IUPAC Name: 5-[(E)-2-[4-[(E)-2-(3-carboxy-4-hydroxyphenyl)ethenyl]phenyl]ethenyl]-2-hydroxybenzoic acid
• InChIKey: MCBNOAYTZBUCSX-KQQUZDAGSA-N
• INCHI: 1S/C24H18O6/c25-21-11-9-17(13-19(21)23(27)28)7-5-15-1-2-16(4-3-15)6-8-18-10-12-22(26)20(14-18)24(29)30/h1-14,25-26H,(H,27,28)(H,29,30)/b7-5+,8-6+
• SMILES isoméricas: C1=CC(=CC=C1/C=C/C2=CC(=C(C=C2)O)C(=O)O)/C=C/C3=CC(=C(C=C3)O)C(=O)O
• PubChem CID: 9930943
• Peso molecular: 402.4

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Phenylpropanoids and polyketides
    • Classe Stilbenes

• Kingdom: Organic compounds
• Superclass: Phenylpropanoids and polyketides
• Classe: Stilbenes
• Subclass: Not available
• Intermediate Tree Nodes: Not available
• Direct Parent: Stilbenes
• Alternative Parents: Salicylic acids Benzoic acids Styrenes Benzoyl derivatives 1-hydroxy-2-unsubstituted benzenoids Vinylogous acids Carboxylic acids Organooxygen compounds Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic homomonocyclic compounds
• Substituents: Stilbene - Salicylic acid - Salicylic acid or derivatives - Hydroxybenzoic acid - Benzoic acid or derivatives - Benzoic acid - Benzoyl - Styrene - 1-hydroxy-2-unsubstituted benzenoid - Phenol - Monocyclic benzene moiety - Benzenoid - Vinylogous acid - Carboxylic acid - Carboxylic acid derivative - Hydrocarbon derivative - Organic oxide - Organic oxygen compound - Organooxygen compound - Aromatic homomonocyclic compound
• Descrição: This compound belongs to the class of organic compounds known as stilbenes. These are organic compounds containing a 1,2-diphenylethylene moiety. Stilbenes (C6-C2-C6 ) are derived from the common phenylpropene (C6-C3) skeleton building block. The introduction of one or more hydroxyl groups to a phenyl ring lead to stilbenoids.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Propriedades químicas e físicas

• Solubilidade: DMSO: 2.0mg/mL