≥98% for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at 2-8°C,Protected from light,Desiccated Ships Wet ice Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Visão geral
ZK 216348 ((+)-ZK 216348) is a nonsteroidal selective glucocorticoid receptor agonist with an IC 50 of 20.3 nM. ZK 216348 also binds to Progesterone and mineralocorticoid receptors with IC 50 s of 20.4 nM and 79.9 nM, respectively. ZK 216348 has antiinflammatory activity similar to Prednisolone and induces less transactivation-mediated side effects
In Vitro
In human peripheral blood mononuclear cells (PBMCs), ZK 216348 inhibits TNF-α and IL-12 with IC 50 of 89 nM and 52 nM, respectively. Participation of an active GR in the antiinflammatory response of ZK 216348 is further investigated in Caco-2 cells, where the TNF-α-induced expression of the proinflammatory cytokine IL-8 is suppressed in the presence of ZK 216348. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
ZK 216348 (1-30 mg/kg; subcutaneous injection; for 24 hours; NMRI mice and Wistar rats) treatment inhibits ear edema in both mice and rats. A markedly superior side-effect profile is found in ZK 216348 with regard to increases in blood glucose, spleen involution, and, to a lesser extent, skin atrophy . MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: NMRI mice (26-28 g) and Wistar rats (140-160 g) injection with Croton oil Dosage: 1 mg/kg, 3 mg/kg, 10 mg/kg and 30 mg/kg Administration: Subcutaneous injection; for 24 hours Result: Inhibited ear edema in mice and rats.
ZK 216348 ((+)-ZK 216348) is a nonsteroidal selective glucocorticoid receptor agonist with an IC 50 of 20.3 nM. ZK 216348 also binds to Progesterone and mineralocorticoid receptors with IC 50 s of 20.4 nM and 79.9 nM, respectively. ZK 216348 has antiinfla
Condições de armazenamento de armazenamento
Store at 2-8°C,Protected from light,Desiccated
Enviado em
Wet ice
Este produto requer transporte de cadeia fria. Serviços terrestres e outros serviços econômicos não estão disponíveis.
This compound belongs to the class of organic compounds known as benzoxazines. These are organic compounds containing a benzene fused to an oxazine ring (a six-membered aliphatic ring with four carbon atoms, one oxygen atom, and one nitrogen atom).
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Revisões
Avaliações dos Clientes
Application Protocols
Item-specific (from Product Data)
No tested applications or recommended conditions are provided.
General research protocols for small molecules (not item-specific)
Stock preparation: Dissolve in anhydrous DMSO to a convenient concentration (e.g., 10 mM). Vortex and, if necessary, sonicate briefly. Filter through a 0.22 µm PTFE membrane if particulates persist and the compound is stable.
Working solutions: Dilute stocks into assay buffer or medium to the desired final concentration, ensuring the final organic cosolvent content remains within acceptable limits for your system (commonly ≤0.5–1% DMSO for cells).
Controls: Include vehicle-only controls and, where applicable, a known reference compound. Perform at least an 8-point dilution series (1:3 or 1:2) for potency curves.
Stability checks: Reassess concentration by LC–UV or LC–MS after storage and after at least one freeze–thaw cycle. Protect from light during all steps, consistent with storage requirements.
Documentation: Record lot number, preparation date, solvent, and confirmed concentration. Attach chromatograms to notebook entries for traceability.
Biological Roles
Item-specific (from Product Data)
No biological target, pathway, or mechanism is provided for ZK 216348.
Guidance and limitations
Without a verified structure or annotated target, no assertions about biological roles, potency, selectivity, or mode of action can be made for this listing. Avoid extrapolating from similarly named compounds unless you have primary references tied to CAS 669073-68-1 and the same lot.
General considerations for small-molecule research compounds (general knowledge)
If used as a putative probe, confirm identity and purity, then independently replicate any reported activity using orthogonal assays (e.g., biochemical vs. cellular readouts) and counter-screens for off-target effects.
Assess compound stability under assay conditions (pH, temperature, light). Photolabile compounds can degrade to active or inactive species, complicating interpretation.
Evaluate nonspecific effects: aggregation, redox cycling, PAINS substructures, and fluorescence interference can confound screening results. Employ detergent controls, dynamic light scattering, and redox/fluorescence interference panels as appropriate.
Record and report the exact batch number, concentration verification (by UV with extinction coefficient or qNMR), and vehicle composition to ensure reproducibility.
Buffer Applications
Applicability assessment
ZK 216348 is a discrete small molecule, not a buffering agent. It does not define a conjugate acid/base pair or a pKa window for buffering in aqueous systems (pKa not provided). Therefore, buffer preparation guidance is generally not applicable.
Practical notes (general knowledge)
If the compound must be delivered in aqueous media for biological assays, use an appropriate research buffer (e.g., PBS, HEPES, or Tris) suited to your biological system, and add the compound from a concentrated organic stock (commonly DMSO) while staying within acceptable cosolvent limits.
Pre-clear buffers by filtration (0.22 µm) and confirm compound stability at the working pH prior to extended incubations.
Avoid strong acids or bases unless the compound’s ionization and stability profile are known; uncontrolled pH shifts can cause hydrolysis or precipitation.
Green Alternatives
Context
The product is a small-molecule research compound rather than a reaction solvent or bulk reagent. “Green alternatives” are therefore most applicable to the solvents and formulation choices used to handle the compound, not to the compound itself.
Greener solvent handling (general knowledge)
Prefer less hazardous, more sustainable solvents where compatible with solubility and assay needs.
Illustrative comparison (general; not item-specific)
DMSO: High solvency, miscible with water; relatively low volatility; widely used for stocks in screening. Environmental profile acceptable but disposal still required.
Ethanol (EtOH): Renewable routes available; good for biological compatibility at low percentages; may not dissolve highly lipophilic compounds at high concentration.
2-MeTHF vs THF: For any synthetic steps or sample workups surrounding this compound, 2-MeTHF is a greener alternative to THF (biobased, lower peroxide formation), but only relevant if you are performing chemistry with this molecule.
CPME vs Et2O: CPME offers lower peroxide propensity and better stability.
Best practices
Minimize DMSO concentration in biological assays (≤0.5–1% v/v) to reduce solvent burden.
Use amber vials and right-sized aliquots to extend solution life and reduce waste.
Employ microscale analytics (UPLC) to cut solvent consumption during QC.
Segregate waste streams and consult institutional green-chemistry guidelines for solvent selection matrices.
Pharmaceutical Uses
Scope and limitations
This product is sold strictly for research use only. No clinical, diagnostic, or therapeutic uses are claimed or permitted.
Formulation-oriented notes (general knowledge; not item-specific)
If the compound is evaluated in pre-formulation research, typical prototype vehicles for in vitro or ex vivo work include DMSO stocks diluted into buffered saline or culture medium. For in vivo exploratory studies (outside the scope of this listing), formulation must follow institutional guidelines and regulatory frameworks; consult the SDS and primary literature first.
Solubility enhancement tactics may include cosolvents (PEG 400, ethanol), surfactants (Tween-80, Cremophor EL), or inclusion complexes (HP-β-CD). Use only in research contexts and verify compatibility with the test system.
Documentation
Pharmacopeial status, impurity limits, and excipient roles are Not specified for this item; refer to CoA/Spec Sheet if applicable. Any use beyond basic research requires comprehensive characterization and regulatory review, which are not provided here.
Physical Properties
Item-specific (from Product Data)
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
Literature/computed (not item-specific)
Boiling point, melting point, density, refractive index, logP, pKa, UV-Vis characteristics: Not reported here. For precise values, consult peer-reviewed references for CAS 669073-68-1 or the lot-specific CoA.
General guidance for small-molecule research compounds (general knowledge)
Solubility screening hierarchy: start with DMSO (analytical grade, water ≤0.1% if moisture sensitive), then evaluate miscible cosolvents (DMF, NMP, AcCN, MeOH, EtOH) and biphasic options (EtOAc, MTBE) as needed. Actual solubility is structure-dependent and must be experimentally verified.
Hygroscopicity/photolability: Storage instructions supplied (2–8°C, protected from light, desiccated) suggest potential sensitivity to moisture and light; minimize exposure during weighing and solution prep.
If crystalline, melting point can aid identity confirmation; if amorphous, DSC/TGA may be preferred. Record observed MP for internal QC but do not treat as specification unless on the CoA.
Quality and Grades
Item-specific (from Product Data)
Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
Guidance on interpreting grades (general knowledge)
Screening/compound library grade: Typically intended for discovery biology/chemistry assays. Documentation often includes identity confirmation (LC–MS, 1H NMR) and purity (HPLC area %) but may not include full elemental analysis.
Analytical/AR grade: Emphasis on defined impurity limits and reproducible performance in analytical workflows.
HPLC grade solvents/reagents: Defined UV cutoffs and low trace contaminants to minimize background in chromatographic detection.
What to verify for this item
Identity confirmation: Request LC–MS and NMR traces aligned to CAS 669073-68-1 and the provided CID.
Purity basis: Ensure the reported purity is method-defined (e.g., HPLC at 254 nm, ELSD). If unstated, treat purity as “Not specified” and assess fitness-for-use in your assay.
Residual solvents and water content: Not specified for this item; refer to CoA/Spec Sheet. Karl Fischer (water) and GC (residual solvents) can be run in-house if necessary.
Stabilizers/inhibitors: None indicated; Not specified for this item—confirm before long-term storage or photolysis studies.
Reaction and Applications
Item-specific (from Product Data)
Manufacturer Applications: Not specified in the listing; no reaction roles are claimed.
Applicability assessment
Without a disclosed structure, ZK 216348 should be treated as a finished small-molecule research tool/ligand candidate rather than a general-purpose reagent or building block. Consequently, classical synthetic applications (e.g., serving as a catalyst, coupling partner, or protecting group reagent) are not typically relevant.
Research usage patterns (general knowledge)
Screening and profiling: Commonly, ZK-designated compounds are evaluated in biochemical and cell-based assays for target engagement, SAR, and ADME profiling. Prepare calibrated DMSO stocks, validate by LC–MS, and include vehicle controls.
Analytical characterization: Use LC–UV/ELSD/MS to confirm purity and stability in your chosen solvent. Photostability should be checked, given the light-protection storage requirement.
Reference standard use: If employed as an internal or external standard, ensure traceability to a lot-specific CoA with purity assignment and, where possible, quantitative NMR (qNMR) content.
Practical tips
Establish a solubility curve across solvents and temperatures prior to high-throughput use.
Run stability-indicating assays (e.g., LC with dual-wavelength UV) after freeze–thaw cycles.
If derivatization or immobilization is planned, obtain the exact functional group map from the structure before attempting conjugations.
Reaction Conditions
Applicability assessment
No synthetic or catalytic role is provided for ZK 216348 in the Product Data. As such, standard “reaction conditions” are not applicable.
General notes (for users who may explore chemistry on this scaffold; not item-specific)
Solvent: Begin with dry, oxygen-free solvents consistent with the functional groups present (unknown here). DMSO/DMF/NMP can be used for SNAr/amide couplings; 2-MeTHF/CPME for hydrophobic transformations when feasible.
Temperature: Screen ambient to 80 °C for solution-phase derivatizations; avoid heating if photolabile or thermally sensitive.
Catalysts/bases: Selection depends entirely on the actual functional groups. Avoid strong bases/nucleophiles until stability is known.
Monitoring: LC–MS is preferred for complex scaffolds. Use UPLC for rapid method development. Include light shielding if the compound is photosensitive.
Expected yields
Not specified; any yields would be literature- and scaffold-dependent. Establish small-scale feasibility studies before scale-up.
Safety and Handling
Item-specific (from Product Data)
Storage: Store at 2–8°C, protected from light, desiccated. Shipped on wet ice.
GHS classification, signal word, pictograms, hazard (H) statements: Not specified for this item; refer to SDS.
General safety guidance (always defer to SDS)
PPE: Lab coat, safety glasses, and nitrile gloves as baseline. Use chemical splash goggles and additional dermal protection when handling concentrated solutions or powders.
Engineering controls: Handle powders/aliquots in a fume hood to avoid inhalation. Employ light shielding (amber glassware/foil) if the compound is photosensitive.
Weighing and transfer: Given the desiccated storage requirement, work rapidly with minimal air exposure. Use pre-dried tools and containers. If electrostatic, consider antistatic measures.
Incompatibilities: Unknown; avoid strong oxidizers/reductants and acids/bases until compatibility is known. Do not heat or irradiate solutions unnecessarily.
First aid (overview): Eye/skin contact—rinse with water for ≥15 min and remove contaminated clothing. Inhalation—move to fresh air; seek medical advice. Ingestion—rinse mouth; do not induce vomiting; seek medical attention. Bring the SDS.
Waste: Collect solutions/solids as halogenated or non-halogenated organic waste per institutional policy until composition is confirmed.
Note: No hazard category provided here. Treat as a potentially harmful research chemical; implement a risk assessment before use.
Solvent Selection
Item-specific constraints
No structure or solubility data are provided. Storage indicates light and moisture sensitivity, which often correlates with better stability in anhydrous, oxygen-free, amberized solutions.
General solvent strategies for small-molecule research compounds (general knowledge)
Stock solutions: Begin with anhydrous DMSO (common starting range 5–50 mM). If insoluble, test DMF, NMP, or mixtures (e.g., DMSO:DMF 1:1). For biochemical assays, keep final DMSO ≤0.5–1% v/v to avoid assay interference.
Aqueous formulations: If water solubility is poor, use co-solvent systems (e.g., 10–40% AcCN, MeOH, or EtOH in buffer) or add cyclodextrins/surfactants (0.1–1% Tween-20) where biocompatible. Titrate pH only if the compound is known to be ionizable (unknown here—verify first to avoid degradation).
Volatile organic options: Acetonitrile, ethyl acetate, and MTBE can be used for workup or intermediate handling; test for stability.
Degassing/inerting: If the compound is air/light sensitive, prepare and store solutions under nitrogen or argon in amber vials.
Comparison (general, not item-specific)
DMSO vs DMF: DMSO is broadly biocompatible for screening; DMF offers higher solubilizing power but is less desirable in live-cell assays.
EtOH vs MeOH: Ethanol is typically preferred for biological systems; methanol may be used for analytical stocks.
Aqueous buffers: Choose after confirming ionization behavior; uncontrolled basification or acidification can trigger hydrolysis or isomerization.
Storage and Reconstitution
Item-specific (from Product Data)
Storage conditions: Store at 2–8°C, protected from light, desiccated.
Shipping: Shipped on wet ice.
Appearance: Not specified for this item; refer to CoA/Spec Sheet.
Practical guidance (general knowledge)
Primary storage: Keep material in the original, tightly closed container with desiccant. Limit headspace and exposure time during handling. Use amber vials or wrap in foil to minimize light exposure.
Aliquoting: Upon first opening, subdivide into single-use aliquots under dry inert gas (N2/Ar) if feasible. This minimizes moisture uptake and repeated freeze–thaw/light exposure.
Reconstitution: If solid, prepare concentrated anhydrous DMSO stock (e.g., 10–50 mM) based on the molecular weight from the CoA. If MW is not available, determine mass balance by qNMR before potency-critical work.
Solution storage: Store DMSO stocks at 2–8°C protected from light for short term. For longer term, consider −20°C in amber vials. Avoid repeated freeze–thaw; thaw gently to room temperature before use and vortex to ensure homogeneity.
Stability: No lot-specific stability data are provided. Verify by LC–MS/LC–UV after 1–2 weeks of storage and after a freeze–thaw cycle.
Labeling: Include compound name, CAS (669073-68-1), concentration, solvent, preparation date, and your initials on each aliquot.
Research Use: For research use only.
Structure and Identity
Item-specific (from Product Data)
Product name: ZK 216348 (SKU: Z651002)
CAS: 669073-68-1
PubChem CID: 9805004
InChIKey: 386876 (as provided; note that complete InChIKey typically has 27 characters — verify on the CoA/SDS)
SMILES: Not specified for this item; refer to CoA/Spec Sheet.
Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
General chemistry notes
“ZK” designations are often used by originators to denote small-molecule research ligands; however, without a provided structure, no assumptions about scaffold, functional groups, stereochemistry, or protonation state should be made.
For any structural work (NMR, LC–MS, HRMS, or crystallography), obtain the definitive structure (SMILES/InChI) from the CoA or primary literature that matches the exact CAS (669073-68-1) and lot.
2D structure description (not item-specific due to missing structure)
Not available. Once structure is confirmed, describe ring systems (e.g., aromatic/heteroaromatic cores), heteroatom count (O/N/S/halogens), stereocenters (R/S), and functional handles (e.g., amide, urea, sulfonamide) to guide solubility and handling.
Synthetic Utility
Applicability assessment
ZK 216348 is listed as a small-molecule library compound. Without a disclosed structure or functional group map, it is not positioned as a synthetic building block or reagent in this catalog entry.
General guidance (not item-specific)
Derivatization campaigns (e.g., SAR expansion, biotinylation, or photoaffinity tag installation) require knowledge of reactive handles (amines, alcohols, halides, carboxyls). Obtain the exact structure (SMILES/InChI) and assess chemoselectivity before attempting modifications.
If used as a reference standard, synthetic utility centers on calibration and QC rather than transformation—use qNMR for potency assignment and LC–MS to monitor degradation products during forced-degradation studies (acid/base, oxidative, photolytic, thermal).
Retrosynthetic considerations
Once the scaffold is known, retrosynthetic planning should focus on convergent assembly of the core, late-stage diversification sites, and stability of protecting groups versus any light/moisture sensitivities implied by storage conditions.
Conclusion
Until structural details are confirmed, treat ZK 216348 as a finished research analyte rather than a reagent for transformations.
Target Specificity
Item-specific (from Product Data)
No biological target, enzyme, receptor, or pathway specificity is provided for ZK 216348.
Guidance
Do not infer target specificity from the “ZK” prefix alone. If used in target-based assays, validate activity with orthogonal methods and appropriate positive/negative controls. Cross-check against the exact CAS (669073-68-1) and lot documentation.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
