GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥85%(SDS-PAGE)expressed in E.coli；≥100 mU/mg protein; Protein concentration: See COA
Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥85%(SDS-PAGE), expressed in E.coli；≥100 mU/mg protein; Protein concentration: See COA ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
β-1,4-Galactosyltransferase (LgtB), EC 2.4.1.90, derived from Neisseria meningitidis.This enzyme transfers galactose via a β‑1,4 linkage to oligosaccharide chains with non‑reducing‑terminal GlcNAc or glucose. It is currently the most efficient enzyme for enzymatic synthesis of LacNAc. Recombinantly expressed in Escherichia coli, this product exhibits high purity and high activity.
Reaction Equation
GlcNAc + UDP‑Gal → LacNAc + UDP
Usage Instructions
Take out the enzyme and dissolve it in an appropriate amount of purified water, mix well and set aside. Do not repeatedly freeze‑thaw the dissolved enzyme; store it at 2–8 °C.
Add substrates and sugar nucleotide donor (UDP‑Gal), 5 mM MnCl₂ to the reaction system. Appropriate buffer salts (e.g., Tris‑HCl) may be supplemented to adjust pH to 6.0, then add an appropriate amount of LgtB according to reaction requirements. Alternatively, UDP‑Gal can be synthesized via a one‑pot multi‑enzyme method by adding enzymes GalK and AtUSP, substrates ATP, UTP, and acceptor substrates. Adjust pH to 6.0 and add an appropriate amount of LgtB according to reaction requirements.
After mixing, incubate the sample at 37 °C for 1–4 hours.
Note: When scaling up the reaction system, adjust incubation time or enzyme dosage according to actual conditions; low‑speed shaking can be used for homogenization.
Precautions
For your safety and health, wear a lab coat and disposable gloves during operation. This product is for research purposes only.
Specifications
Product Name
β-1,4-Galactosyltransferase (LgtB), CAS No.9054-94-8
ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™
Especificaciones y pureza
Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥85%(SDS-PAGE), expressed in E.coli；≥100 mU/mg protein; Protein concentration: See COA
Bioactividad
≥100 mU/mg protein
Peso molecular previsto
35 kDa
CAS
9054-94-8
Número de la Comisión de Enzimas
EC 2.4.1.90
Tipo de molécula
Enzyme
Almacenamiento y envío
Concentración
expressed in E.coli；≥100 mU/mg protein; Protein concentration: See COA
Condiciones de almacenamiento de almacenamiento
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Definición de unidad
The amount of enzyme required to transfer 1 μmol of Gal from UDP‑Gal to GlcNAc to form Galβ1‑4GlcNAc per minute at 37 °C.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
