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Editors' picks this month
Hand-selected by our scientific team — the most useful reads right now.
Eight Common Lysis Buffers
In handling cell and tissue samples, choosing a lysis buffer is not just about “breaking cells open”—it determines whether proteins are detectable, remain active, and retain complexes and post-translational modifications. Different systems vary greatly in strength, denaturation, and effects on membranes and nuclei; the protein profiles you obtain from the same sample can differ markedly under different lysis conditions.
Polyethylene Glycol (PEG): Properties, Applications Across Multiple Fields, and Product Selection Guide
Polyethylene glycol (PEG) is a family of linear polyether polymers produced by the stepwise addition polymerization of ethylene oxide with water or ethylene glycol.
What is Ion Exchange Chromatography Used For? 4 Important Applications for Protein Purification
Ion exchange chromatography is an adsorption chromatography technique that separates molecules based on differences in net surface charge, and it plays a central role in the research, development, and production of proteins, biomacromolecules, and biopharmaceuticals. Under appropriate pH conditions, protein surfaces carry different numbers and types of charges. Through electrostatic adsorption between these charges and fixed charged groups on the chromatographic resin, the target protein can be selectively captured while impurities with different charge characteristics are separated. Owing to this mechanism, ion exchange chromatography is suitable not only for efficient purification of natively folded proteins, but also for separating post-translationally modified variants, conformational heterogeneity, and subtypes with different oligomeric states. It is an important tool for studying protein structure–function relationships, establishing critical quality attributes, and evaluating formulation homogeneity.
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Technical Articles: Reagent Chemistry, Synthesis & Bioscience Guides
18751,700+ technical articles by Aladdin Scientific's scientists on reagent selection, reaction mechanisms, assay design and formulation. New every week.
- Does GIPR-Mediated Weight Loss Depend on Agonism or Antagonism?
- –SO₂CH₃Direct Construction of Methylsulfonyl-Containing Heterocycles: Synthesis and Applications of Prefunctionalized Three-Carbon Vinamidinium Salts
- Organic synthesis6-Chloro-2-methyl-2,3-dihydro-1H-inden-1-one: Properties & Applications
- SqualaneStructure, Emollient and Moisturizing Effects, and Source Identification of Squalane: From Complete Hydrogenation of Squalene to Stable Isotope Analysis
- coenzyme Q10Structural and Functional Differences Between Idebenone and Coenzyme Q10: From Side-Chain Structure to Redox Cycling and Mitochondrial Electron Transport
Laboratory Protocols & SOPs Library
32283,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
- Cyclic nucleotidesDibutyryl-Cyclic GMP (Sodium Salt): Lab Use Guidelines
- H3TATBH3TATB MOF Synthesis Protocol: Step-by-Step Guide
- β-Propiolactoneβ-Propiolactone (BPL) Viral Inactivation — Standard Operating Procedure (SOP)
- MSCsStandard Operating Procedure for Adipogenic Differentiation of Mesenchymal Stem Cells and Evaluation by Oil Red O Staining
- Other experimentsExperimental purification of λ phage particles by precipitation/centrifugation
Frequently Asked Questions: Products, Experiments and Troubleshooting
300Answers from Aladdin Scientific's technical team to 290+ questions on antibodies, reagents, purity, storage and experiment troubleshooting.
- cell cultureTrypan Blue Solution (0.2%): FAQs for Cell Viability
- Peptide PurityDetermination of Peptide Purity
- Meet AldaMeet Alda, Aladdin Scientific’s Digital Product Assistant
- Flow-through AntibodiesIs the mouse Fc blocker in μg packaging and is it powdery in nature? Does it dissolve in pbs?
- Flow-through AntibodiesWhat is the difference between our Flow Antibodies available in Test packages and μg packages?
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Reagent Specifications, Grading and Purity
194What reagent grades, purity labels and specifications mean: ACS/AR, HPLC, electronic grade and tag-grade proteins, in 190+ guides from Aladdin Scientific.
- GradeWhat Grade Is Purum? From Grade Meaning and Specification Interpretation to Reagent Selection
- Grade“Tissue Engineering Grade” Reagents and Materials: Grade Meaning, Critical Quality Attributes, and Selection Methods
- COAReagent Purity Labeling System and Interpretation Principles
- Electronic GradeFrom EL to UPSSS: Cleanliness Grades, Key Specifications, and Selection Logic for Electronic-Grade Reagents
- AVI tagAVI-Tag Grade Reagents
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