3-Amino-9-ethylcarbazole - ≥95% , CAS No.132-32-1

CAS: 132-32-1 Cat. No.: A105545 Peso molecular: 210.28 Beilstein Registry Number: 22(4)4936 Número EC: 205-057-7

GRADE & PURITY ≥95%

Synonyms

9-ethyl-3-carbazolamine | CHEBI:122149 | AI3-50580 | 3-AMINO-9-ETHYLCARBAZOLE [HSDB] | 9H-Carbazol-3-amine, 9-ethyl- | 3-AMINO-9-ETHYLCARBAZOLE | 3-Amino-9-ethylcarbazole, Technical | 8Q2BG27JBU | Carbazole, 3-amino-9-ethyl- | SCHEMBL37700 | SY057088 | F0

Storage

Store at 2-8°C,Argon charged

Shipped In

Wet ice

Descripción general

AEC, 50X (3-amino-9-ethylcarbazole); for HRP-based detection systems; provides red chromogen depositions. Precipitable peroxidase substrate for immunohistochemical (IHC) staining techniques. In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product. Although not as sensitive as diaminobenzidine (DAB, 50X;, the red deposit formed at the sites of AEC oxidation make this substrate useful for double labeling. Aqueous-based mounting medium and counterstains must be used for IHC applications since oxidized AEC is soluble in alcohols and xylene (e.g., Crystal Mounting Medium; ). Use with AEC Substrate Buffer .
Substrate for peroxidase. Usage Prepare samples and incubate with antibodies and HRP-labeled probes, follow with all necessary wash steps. ? Prepare 1X AEC Substrate Buffer (dilute AEC Substrate Buffer, 10X;). ? Dilute one part AEC, 50X with 50 parts 1X AEC Substrate Buffer to make 1X AEC Substrate Solution. Mix well. ? Cover tissue sections with 1X AEC Substrate Solution and incubate 10-30 min. at RT. ? Rinse thoroughly with distilled water. If desired, follow with an appropriate aqueous-based counterstain. ? Mount tissue samples with an aqueous-based mounting medium (e.g., Crystal Mounting Medium; ) ? Unused AEC solution can be discarded according to city, county, state, province or country’s regulations

Specifications

Sinónimos

9-ethyl-3-carbazolamine | CHEBI:122149 | AI3-50580 | 3-AMINO-9-ETHYLCARBAZOLE [HSDB] | 9H-Carbazol-3-amine, 9-ethyl- | 3-AMINO-9-ETHYLCARBAZOLE | 3-Amino-9-ethylcarbazole, Technical | 8Q2BG27JBU | Carbazole, 3-amino-9-ethyl- | SCHEMBL37700 | SY057088 | F0

Especificaciones y pureza

≥95%

Mecanismos bioquímicos y fisiológicos

3-Amino-9-ethylcarbazole is a peroxidase substrate suitable for use in immunoblotting and immunohistochemical staining procedures. This substrate produces an insoluble end product that is red in color and can be observed visually.Peroxidase substrate. Ins

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C, Argon charged

Enviado en

Wet ice

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Nota

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Pureza

≥95%

Nombres e identificadores

• Pubchem Sid: 488180837
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/488180837
• Sonrisas canónicas: CCN1C2=C(C=C(C=C2)N)C3=CC=CC=C31
• IUPAC Name: 9-ethylcarbazol-3-amine
• InChIKey: OXEUETBFKVCRNP-UHFFFAOYSA-N
• INCHI: 1S/C14H14N2/c1-2-16-13-6-4-3-5-11(13)12-9-10(15)7-8-14(12)16/h3-9H,2,15H2,1H3
• Isómeros SMILES: CCN1C2=C(C=C(C=C2)N)C3=CC=CC=C31
• RTECS: FE3590000
• Número ONU: 2811
• Grupo de embalaje: I
• Peso molecular: 210.28
• Beilstein: 22(4)4936
• Reaxy-Rn: 174969
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=174969&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Clase Indoles and derivatives
      • Subclass Carbazoles

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Clase: Indoles and derivatives
• Subclass: Carbazoles
• Intermediate Tree Nodes: Not available
• Direct Parent: Carbazoles
• Alternative Parents: N-alkylindoles Indoles Substituted pyrroles Benzenoids Heteroaromatic compounds Azacyclic compounds Primary amines Organopnictogen compounds Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Carbazole - N-alkylindole - Indole - Benzenoid - Substituted pyrrole - Heteroaromatic compound - Pyrrole - Azacycle - Organic nitrogen compound - Organopnictogen compound - Hydrocarbon derivative - Primary amine - Organonitrogen compound - Amine - Aromatic heteropolycyclic compound
• Descripción: This compound belongs to the class of organic compounds known as carbazoles. These are compounds containing a three ring system containing a pyrrole ring fused on either side to a benzene ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Objetivos asociados (humanos)

ALOX15B Tchem Arachidonate 15-lipoxygenase, type II (7244 Activities)

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ALDH1A1 Tchem Aldehyde dehydrogenase 1A1 (77053 Activities)

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HBB Tbio Hemoglobin beta chain (329 Activities)

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SMPD1 Tchem Sphingomyelin phosphodiesterase (13561 Activities)

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GLP1R Tclin Glucagon-like peptide 1 receptor (111429 Activities)

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HSD17B10 Tchem Endoplasmic reticulum-associated amyloid beta-peptide-binding protein (20669 Activities)

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HTT Tchem Huntingtin (19182 Activities)

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KDM4A Tchem Lysine-specific demethylase 4A (52245 Activities)

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EHMT2 Tchem Histone-lysine N-methyltransferase, H3 lysine-9 specific 3 (93046 Activities)

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TDP1 Tchem Tyrosyl-DNA phosphodiesterase 1 (345557 Activities)

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KDM4E Tchem Lysine-specific demethylase 4D-like (40243 Activities)

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HPGD Tchem 15-hydroxyprostaglandin dehydrogenase [NAD+] (24926 Activities)

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GMNN Tbio Geminin (128009 Activities)

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CBX1 Tbio Chromobox protein homolog 1 (92434 Activities)

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ATXN2 Tbio Ataxin-2 (54410 Activities)

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IDH1 Tclin Isocitrate dehydrogenase [NADP] cytoplasmic (40980 Activities)

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MEN1 Tchem Menin/Histone-lysine N-methyltransferase MLL (48157 Activities)

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RUNX1 Tbio Runt-related transcription factor 1/Core-binding factor subunit beta (7867 Activities)

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RACGAP1 Tbio Rac GTPase-activating protein 1 (2057 Activities)

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Objetivos asociados (no humanos)

Cruzipain (33337 Activities)

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Mapk1 MAP kinase ERK2 (650 Activities)

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Salmonella typhimurium (15756 Activities)

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Plasmodium falciparum (966862 Activities)

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Plasmodium berghei (192651 Activities)

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Txnrd1 Thioredoxin reductase 1, cytoplasmic (45279 Activities)

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Mapt Microtubule-associated protein tau (6 Activities)

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Impa1 Inositol monophosphatase 1 (16203 Activities)

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FTL Ferritin light chain (43324 Activities)

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NS1 Nonstructural protein 1 (33327 Activities)

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clpP ATP-dependent Clp protease proteolytic subunit (245 Activities)

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Mecanismos de acción

Propiedades químicas y físicas

• Punto de fusión (°C): 98°C-100°C
• Peso molecular: 210.270 g/mol
• XLogP3: 3.000
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 1
• Rotatable Bond Count: 1
• Exact Mass: 210.116 Da
• Monoisotopic Mass: 210.116 Da
• Topological Polar Surface Area: 31.000 Ų
• Heavy Atom Count: 16
• Formal Charge: 0
• Complexity: 253.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

Referencias

1. Hongxia Xiu, Yajie Liu, Huihui Yang, Haibin Ren, Bowen Luo, Zhipeng Wang, Hong Shao, Fengzhong Wang, Jingjian Zhang, Yutang Wang.  (2022)  Identification of novel umami molecules via QSAR models and molecular docking.  Food & Function,  13  (14): (7529-7539).  [PMID:35765918] [10.1039/D2FO00544A]

2. Jinmao You, Kun Dou, Cuihua Song, Guoliang Li, Zhiwei Sun, Shijuan Zhang, Guang Chen, Xianen Zhao, Na Hu, Wu Zhou.  (2017)  3-(2-Bromoacetamido)-N-(9-ethyl-9H)-carbazol fluorescent probe and its application for the determination of thiophenols in rubber products by HPLC with fluorescence detection and atmospheric chemical ionization mass spectrometry identification.  JOURNAL OF SEPARATION SCIENCE,  40  (12): (2528-2540).  [PMID:28371096] [10.1002/jssc.201601166]

3. Chunyan Qin, Wei Wen, Xiuhua Zhang, Haoshuang Gu, Shengfu Wang.  (2015)  Visual detection of thrombin using a strip biosensor through aptamer-cleavage reaction with enzyme catalytic amplification.  ANALYST,  140  (22): (7710-7717).  [PMID:26451394] [10.1039/C5AN01712B]

4. Xianqing Tang, Hua Zhang, Jinghuang Chen, Donghui Wu, Yu Wang, Jian Sun, Zhenxin Wang, Xiurong Yang.  (2025)  Ferrous Tungstate Nanomaterials with Excellent Enzyme-Mimicking Activity to Enhance Lateral Flow Immunoassay Sensitivity.  ANALYTICAL CHEMISTRY,      [PMID:39884848] [10.1021/acs.analchem.4c04761]

5. Xiaoyu Qin, Wenhao Guo, Yining Wang, Xiangdong Cai, Jianxi Xiao.  (2025)  A Pathological Collagen-Targeting Nanozyme Chip for Ultrasensitive Quantification of Collagen in Body Fluids.  ANALYTICAL CHEMISTRY,      [PMID:41081707] [10.1021/acs.analchem.5c04014]