GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Descripción general
In this method, ethylidene-blocked p-nitrophenyl maltoside is used as the substrate. α-Amylase hydrolyzes it to ethylidene maltosaccharides (Et-Gx) and p-nitrophenyl maltosaccharides (Gy-pNP). Gy-pNP is further hydrolyzed by α-glucosidase to release p-nitrophenol (p-NP). The final product of this cascade reaction, p-NP, exhibits a characteristic absorbance peak at 405 nm, and its rate of absorbance increase is proportional to the α-amylase activity in the sample. By continuously monitoring the initial linear increase in OD₄₀₅ per unit time (ΔOD₄₀₅/min) in kinetic mode, the α-amylase activity in the sample can be precisely calculated.
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C
Enviado en
Wet ice
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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