Aladdin® 594 NHS Ester (Succinimidyl Ester) - Ex:590nm, Em:618nm, mixture of isomers , CAS No.295348-87-7(free base)

CAS: 295348-87-7(free base) Cat. No.: A729269 Peso molecular: 819.85(free base)

GRADE & PURITY Ex:590nm, Em:618nm, mixture of isomers

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Descripción general

Aladdin® 594 is a bright red dye. Used for stable signal generation in imaging and flow cytometry, Aladdin® 594 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Aladdin® 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. 

The NHS ester (or succinimidyl ester) of Aladdin® 594 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Aladdin® conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores. 

Detailed information about this Aladdin® NHS ester:

Fluorophore label: Aladdin® 594 dye

Reactive group: NHS ester

Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides

Ex/Em of the conjugate: 590/617 nm

Extinction coefficient: 92,000 cm-1M-1

Molecular weight: 819.8

The Aladdin® NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO), and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour. Because the pKa of the terminal amine is lower than that of the lysine epsilon-amino group, you may achieve more selective labeling of the amine terminus using a buffer closer to neutral pH.

Specifications

Especificaciones y pureza

Ex:590nm, Em:618nm, mixture of isomers

Estabilidad y almacenamiento

Store in freezer (-20 ℃) and protect from light.

Condiciones de almacenamiento de almacenamiento

Protected from light, Store at -20°C, Desiccated, Avoid repeated freezing and thawing

Enviado en

Ice chest + Ice pads

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Grado

Em:618nm, Ex:590nm

Nombres e identificadores

• Peso molecular: 819.85(free base)

Propiedades químicas y físicas

• Solubilidad: soluble in water, DMSO, DMF
• Sensibilidad: Light sensitive