Aladdin Fluor 790 NHS Ester - Ex:784nm, Em:814nm , CAS No.A638848

CAS: A638848 Cat. No.: A638848

GRADE & PURITY Ex:784nm, Em:814nm

Storage

Protected from light,Store at -20°C

Shipped In

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Descripción general

Aladdin Fluor 790 is a bright and photostable near-IR dye that is spectrally similar to indocyanine green (ICG) and the IRDye™ 800 dye. Used for stable signal generation in imaging and flow cytometry, Aladdin Fluor 790 dye is water soluble and pH-insensitive from pH 4 to pH 10. Fluorescence of this long-wavelength Aladdin Fluor dye is not visible to the human eye but is readily detected by most imaging systems. As the longest-wavelength Aladdin Fluor dye, the emission is well separated from commonly used far-red fluorophores such as Aladdin Fluor 647 dye or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also expected to be useful for small animal in-vivo imaging (SAIVI) applications or for two-color western applications with the LI-COR™ Odyssey™ infrared imaging system. In addition to reactive dye formulations, we offer Aladdin Fluor 790 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.The NHS ester (or succinimidyl ester) of Aladdin Fluor 790 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH₂) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. 

Detailed information about this Aladdin Fluor NHS ester:

Fluorophore label: Aladdin Fluor 790 dye
Reactive group: NHS ester
Reactivity: Primary amines on proteins and ligands, amine-modified oligonucleotides
Ex/Em of the conjugate: 784/814 nm
Extinction coefficient: 260,000 cm-1M-1
Molecular weight: ∼1750

Typical Conjugation Reaction
You can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies). You can scale the reaction for any amount of protein, but the concentration of the protein should be at least 2 mg/mL for optimal results. We recommend trying three different degrees of labeling, using three different molar ratios of the reactive reagent to protein.

The Aladdin Fluor NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO) , and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour. Because the pKa of the terminal amine is lower than that of the lysine epsilon-amino group, you may achieve more selective labeling of the amine terminus using a buffer closer to neutral pH.

Conjugate Purification
Labeled antibodies are typically separated from free Aladdin Fluor dye using a gel filtration column. For much larger or smaller proteins, select a gel filtration media with an appropriate molecular weight cut-off or purify by dialysis. We offer several purification kits optimized for different quantities of antibody conjugate:
Antibody Conjugate Purification Kit for 0.5-1 mg ()
Antibody Conjugate Purification Kit for 20-50 µg ()
Antibody Conjugate Purification kit for 50-100 µg ()

Specifications

Especificaciones y pureza

Ex:784nm, Em:814nm

Condiciones de almacenamiento de almacenamiento

Protected from light, Store at -20°C

Enviado en

Ice chest + Ice pads

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Grado

Em:814nm, Ex:784nm

Propiedades del producto

• Reactividad: Amine

Propiedades químicas y físicas

• Sensibilidad: Hygroscopic