GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
WB: HeLa, MCF-7, HepG2, NIH3T3 and C6 whole cell lysates Flow Cytometry: Hela cells IF/ICC: Hela cells
Conjugación
Biotin
Grado
ExactAb™, Validated
Tipo de cadena ligera
kappa
Método de purificación
Protein A purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Imágenes
alpha Tubulin Mouse mAb (Biotin) (Ab219159) – IF/ICC IF analysis of alpha Tubulin (red) in Hela cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes and permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were then stained with alpha Tubulin Mouse mAb (Biotin) (Ab219159) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (AF647) (rp181946) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
alpha Tubulin Mouse mAb (Biotin) (Ab219159) - Western Blot All lanes: alpha Tubulin Mouse mAb (Biotin) (Ab219159) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: SA-HRP (np156148) at 1/10000 dilution
Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 120.0 s
alpha Tubulin Mouse mAb (Biotin) (Ab219159) - Flow Cytometry Flow cytometry analysis of alpha Tubulin expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with alpha Tubulin Mouse mAb (Biotin) (Ab219159) (red) at 1/50 dilution for 30 minutes at 4°C in the dark. Then incubated with Streptavidin protein (FITC) (rp155876) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
Aplicación
Aplicación
Dilution info
IF/ICC
1/100-1/1000
WB
1/100-1/1000
Flow Cytometry
1/50
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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