Immobilized Cephalexin Acylase, CAS No.9014-06-6

CAS: 9014-06-6 Cat. No.: A1435573 Número EC: 232-753-8

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, >50 U/g wet weight

Bioactivity

>200 U/g wet weight

Descripción general

 It produces cephalexin from 7-ADCA (7-amino-3-deace-toxy-cephalosporanic acid) and PGM (phenylglycine methyl ester).

Standard

Activity: >200 U/g wet weight

Size of immobilization support: 250~500μm (>95%)

Water content <65%

Enzyme assay: One unit (U) represents the amount of enzyme required for producing 1μmol cephalexin per min in 50mM K-phosphate buffer containing 40mM 7-ADCA (7-amino-3-deace-toxy-cephalosporanic acid) and 40mM PGM (phenylglycine methyl ester), pH 7.0 at 28°C.

Application 

Cephalexin production from 7-ADCA and PGM.

Characteristics 

· High production of cephalexin

· Low degradation activity of cephalexin and PGM

· Fast enzyme reaction (reaction completed in 90 minutes)

· High stability (reusable more than 500 cycles)

· High production rate of cephalexin (more than 98.5%)

Scheme for enzyme reaction 

Optional reaction conditions (Temperature, pH) 

Production instruction

Category	Contents
Materials	·Synthesizing enzyme: Immobilized Cephalexin Acylase (200U/g wet, 61% water content) ·7-ADCA , PGM·HCl ·Water ·Ammonia hydroxide(28%)
Condition	·pH: 7.5-8.0 ·Temperature: 10°C ·7-ADCA : PGM: 350mM : 392mM(1:1.10) ·Enzyme: 1.0 fold of 7-ADCA ·Reaction solution: Water
Step	1. Remove the water after washing immobilized enzyme with water more than five times. 2. Dilute 7-ADCA(350mM) and PGM·HCl (392mM) with 90ml water and adjust pH to 7.5 withammonium hydroxide. 3. Pour 6.75g wet enzyme in conversion reactor. 4. Proceed synthetic reaction at 10°C and 550rpm after pouring substrates. 5. Extract every 10 minutes after 1 hour. Stop the reaction if conversion rate of 7-ADCA is more than 98.5%. 6. Take the reaction solution in reactor.

Fig 3. Cefradine production by Immobilized Cephalexin Acylase

Specifications

Product Name

Immobilized Cephalexin Acylase, CAS No.9014-06-6

Sinónimos

Cephalexin acylase (Immobilized)

Grado

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Especificaciones y pureza

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, >50 U/g wet weight

Bioactividad

>200 U/g wet weight

CAS

9014-06-6

Número de la Comisión de Enzimas

EC 3.5.1.11

Tipo de molécula

Enzyme

Almacenamiento y envío

Concentración

>50 U/g wet weight

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C

Enviado en

Wet ice

Estabilidad y almacenamiento

Store at 2-8℃ long term (24 months). Upon receipt, it is recommended to aliquot.

Definición de unidad

One unit (U) represents the amount of enzyme required for producing 1μmol cephalexin per min in 50mM K-phosphate buffer containing 40mM 7-ADCA (7-amino-3-deace-toxy-cephalosporanic acid) and 40mM PGM (phenylglycine methyl ester), pH 7.0 at 28°C.