Biological stain Biological Stain for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Ships Normal Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 16 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Aniline Blue is the traditional name applied to anionic dyes produced by successive phenylation and sulfonation of basic fuchsine. Aniline blue consists of sulfonation products of variable mixtures of phenylated rosaniline and pararosaniline. It is a mixture of methyl blue and water blue. The dye is also known as cotton blue, and ink blue. Methyl blue of aniline blue belongs to the group of triaminotriphenylmethane dyes.
| Pubchem Sid | 504773448 |
|---|---|
| Pubchem Sid Url | https://pubchem.ncbi.nlm.nih.gov/substance/504773448 |
| Sonrisas canónicas | CC1=CC(=CC(=C1N)S(=O)(=O)[O-])C(=C2C=CC(=NC3=CC=C(C=C3)S(=O)(=O)O)C=C2)C4=CC=C(C=C4)NC5=CC=C(C=C5)S(=O)(=O)[O-].[Na+].[Na+] |
| IUPAC Name | disodium;2-amino-3-methyl-5-[[4-(4-sulfonatoanilino)phenyl]-[4-(4-sulfophenyl)iminocyclohexa-2,5-dien-1-ylidene]methyl]benzenesulfonate |
| InChIKey | XOSXWYQMOYSSKB-UHFFFAOYSA-L |
| INCHI | 1S/C32H27N3O9S3.2Na/c1-20-18-23(19-30(32(20)33)47(42,43)44)31(21-2-6-24(7-3-21)34-26-10-14-28(15-11-26)45(36,37)38)22-4-8-25(9-5-22)35-27-12-16-29(17-13-27)46(39,40)41;;/h2-19,34H,33H2,1H3,(H,36,37,38)(H,39,40,41)(H,42,43,44);;/q;2*+1/p-2 |
| Isómeros SMILES | CC1=CC(=CC(=C1N)S(=O)(=O)[O-])C(=C2C=CC(=NC3=CC=C(C=C3)S(=O)(=O)O)C=C2)C4=CC=C(C=C4)NC5=CC=C(C=C5)S(=O)(=O)[O-].[Na+].[Na+] |
| CAS alternativo | 8004-91-9 |
| Peso molecular | 737.72 |
| Reaxy-Rn | 60338171 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=60338171&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Taxonomy Tree
| Kingdom | Organic compounds |
|---|---|
| Superclass | Benzenoids |
| Clase | Benzene and substituted derivatives |
| Subclass | Diphenylmethanes |
| Intermediate Tree Nodes | Not available |
| Direct Parent | Diphenylmethanes |
| Alternative Parents | Benzenesulfonic acids and derivatives Benzenesulfonyl compounds 1-sulfo,2-unsubstituted aromatic compounds Aniline and substituted anilines Aminotoluenes Sulfonyls Secondary ketimines Organosulfonic acids Azomethines Secondary amines Propargyl-type 1,3-dipolar organic compounds Primary amines Organopnictogen compounds Organic sodium salts Organic oxides Hydrocarbon derivatives |
| Molecular Framework | Aromatic homomonocyclic compounds |
| Substituents | Diphenylmethane - Benzenesulfonate - Benzenesulfonyl group - 1-sulfo,2-unsubstituted aromatic compound - Arylsulfonic acid or derivatives - Aminotoluene - Aniline or substituted anilines - Toluene - Sulfonyl - Organosulfonic acid - Organosulfonic acid or derivatives - Organic sulfonic acid or derivatives - Secondary ketimine - Azomethine - Ketimine - Organic alkali metal salt - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Secondary amine - Organic nitrogen compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Organic sodium salt - Organic salt - Primary amine - Organosulfur compound - Organonitrogen compound - Imine - Amine - Aromatic homomonocyclic compound |
| Descripción | This compound belongs to the class of organic compounds known as diphenylmethanes. These are compounds containing a diphenylmethane moiety, which consists of a methane wherein two hydrogen atoms are replaced by two phenyl groups. |
| External Descriptors | Not available |
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 D2401056 | Certificate of Analysis | Nov 25, 2021 | A113197 |
| E2311037 | Certificate of Analysis | Nov 25, 2021 | A113197 |
| I2318180 | Certificate of Analysis | Nov 25, 2021 | A113197 |
| L2214722 | Certificate of Analysis | Nov 25, 2021 | A113197 |
| Peso molecular | 737.700 g/mol |
|---|---|
| XLogP3 | |
| Hydrogen Bond Donor Count | 3 |
| Hydrogen Bond Acceptor Count | 12 |
| Rotatable Bond Count | 6 |
| Exact Mass | 737.055 Da |
| Monoisotopic Mass | 737.055 Da |
| Topological Polar Surface Area | 244.000 Ų |
| Heavy Atom Count | 49 |
| Formal Charge | 0 |
| Complexity | 1520.000 |
| Isotope Atom Count | 0 |
| Defined Atom Stereocenter Count | 0 |
| Undefined Atom Stereocenter Count | 0 |
| Defined Bond Stereocenter Count | 0 |
| Undefined Bond Stereocenter Count | 0 |
| The total count of all stereochemical bonds | 0 |
| Covalently-Bonded Unit Count | 3 |
| 1. Suping Li, Jingyu Hu, Shiqi Ning, Wei Li, Ru Jiang, Jianguo Huang, Yong Li. (2023) Bacillus velezensis HY19 as a sustainable preservative in post-harvest citrus (Citrus reticulata Blanco L.) fruit management. FOOD CONTROL, [PMID:] [10.1016/j.foodcont.2023.110068] |
| 2. XingYuan Ma, JianFeng Li. (2023) Preparation of full-color carbon quantum dots with multiple emission centers. Materials Today Communications, [PMID:] [10.1016/j.mtcomm.2023.106561] |
| 3. Wang Junjie, Chen Jinhao, Wang Wenwen, Lu Jing, Li Yingying, Bai Ting, Chen Jiahui, Zhu Zongmin, Wang Dong. (2023) Preparation of boron-containing chitosan derivative and its application as intumescent flame retardant for epoxy resin. CELLULOSE, 30 (7): (4663-4681). [PMID:] [10.1007/s10570-023-05174-z] |
| 4. Fenghua Xu, Yi Cheng, Guangcong Ruan, Liqin Fan, Yuting Tian, Zhifeng Xiao, Dongfeng Chen, Yanling Wei. (2021) New pathway ameliorating ulcerative colitis: focus on Roseburia intestinalis and the gut-brain axis. Therapeutic Advances in Gastroenterology, [PMID:33948112] [10.1177/17562848211004469] |
| 5. Songwei Li, Yijie Dong, Lin Li, Yi Zhang, Xiufen Yang, Hongmei Zeng, Mingwang Shi, Xinwu Pei, Dewen Qiu, Qianhua Yuan. (2019) The Novel Cerato-Platanin-Like Protein FocCP1 from Fusarium oxysporum Triggers an Immune Response in Plants. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 20 (11): (2849). [PMID:31212693] [10.3390/ijms20112849] |
| 6. Yan Xuechao, Zhang Xiaoping, Li Qian. (2018) Preparation and characterization of CS/β-CD/Nano-ZnO composite porous membrane optimized by Box-Behnken for the adsorption of Congo red. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 25 (22): (22244-22258). [PMID:29804255] [10.1007/s11356-018-2110-3] |
| 7. Jian-Dong Cui, Ji Qing Qiu. (2012) Production of Extracellular Water-Insoluble Polysaccharide from Pseudomonas sp.. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:22533491] [10.1021/jf3006273] |
| 8. Yao Xu, Xin Tang, Yun Xing, Ziyu Pu, Chengshuang Ling, Qing Yang, Xiaoli Xiong. (2024) 3D hierarchical flowerball-like CoSe2/MnSe nanosheet arrays as a novel electrode for electrochemical sensing of hydrazine. MICROCHEMICAL JOURNAL, [PMID:] [10.1016/j.microc.2024.110504] |
| 9. Ke Ning, Junzhi Li, Xiaoyan Yang, Yuanyuan Xie, Rong Pan, Wei Sun, Ling Yu. (2024) Microfluidic device-assisted 3D cell spheroids isolation, staining and embedding. MICROCHEMICAL JOURNAL, [PMID:] [10.1016/j.microc.2024.111458] |
| 10. Feng-Hua Xu, Xiao Sun, Jun Zhu, Ling-Yang Kong, Yuan Chang, Ning Li, Wen-Xiang Hui, Cong-Peng Zhang, Yi-Ming Cheng, Wen-Xin Han, Zhi-Min Tian, Yan-Ning Qiao, Dong-feng Chen, Lei Liu, Da-Yun Feng, Jing Han. (2025) Significance of the gut tract in the therapeutic mechanisms of polydopamine for acute cerebral infarction: neuro-immune interaction through the gut-brain axis. Frontiers in Cellular and Infection Microbiology, [PMID:40104260] [10.3389/fcimb.2024.1413018] |
| 11. Jinyao Yin, Xuehuan Zhu, Yalong Chen, Yanyang Lv, Jiaxin Shan, Yuhan Liu, Wenbo Liu, Weiguo Miao, Xiao Li. (2025) The Exocyst Subunits EqSec5 and EqSec6 Promote Powdery Mildew Fungus Growth and Pathogenicity. Journal of Fungi, 11 (1): (73). [PMID:39852492] [10.3390/jof11010073] |
| 12. Shan Chen, Yang Zheng, Qing Wang, Rong Mu, Xianchao Sun, Guanhua Ma, Liezhao Liu, Jiequn Ren, Kuo Huang, Guokang Chen. (2025) Harnessing Streptomyces for the Management of Clubroot Disease of Chinese Cabbage (Brassica rapa subsp. Pekinensis). Plants-Basel, 14 (14): (2195). [PMID:40733432] [10.3390/plants14142195] |
| 13. Junyan Liang, Yang Zou, Haiyan Ju, Yonggang Lv. (2025) Rapid angiogenic hydrogel nanofiber scaffold promotes random flap regeneration and modulates inflammation. WOUND REPAIR AND REGENERATION, 33 (2): (e70028). [PMID:40231635] [10.1111/wrr.70028] |
| 14. Jieyi Long, Zhonghui Qiao, Yuan Wang, Andong Zhou, Yibing Wang, Mengshan Zhang, Yong Zhou, Kevin H. Mayo, Lin Sun, Yifa Zhou. (2025) Examining Molecular Biodiversity Using Aniline Blue to Detect β-1,3-Glucans. CHEMISTRY & BIODIVERSITY, [PMID:41115155] [10.1002/cbdv.202501557] |
| 15. Yaping Yue, Tingting Lu, Xiaoyuan Guo, Biao Liu, Shiteng Lv, Heba A. M. Abdalla, Xuelei Lai, Ruihui Zhang, Jianpin Guo, Yu Zhao, Dao-Xiu Zhou, Shaoli Zhou. (2025) ADA2 Forms Nuclear Condensates with GCN5 and ATP-Citrate Lyase (ACL) to Modulate H3K9 Acetylation at Genes Functioning in Rice Meristems. Advanced Science, [PMID:41221643] [10.1002/advs.202513169] |
| 16. Shuai Zhao, Ye Xue, Ruiyao Chen, Xiangyi Ge, Ning An, Yawei Shi. (2026) Revealing an environmentally friendly process for degradation of walnut green husk by Klebsiella variicola M6 and β-xylosidase: Characterization and application. LWT-FOOD SCIENCE AND TECHNOLOGY, [PMID:] [10.1016/j.lwt.2026.119245] |
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View Biological Stain grade guide →
