The Murine Dendritic Cell Cytokine Package contains IL-4 and GM-CSF, key components required for the culturing of murine dendritic cells. Recombinant murine IL-4 is an essential cytokine used in the differentiation and maturation of murine dendritic cell in vitro. The IL-4 protein, specifically recombinant IL-4, promotes the development of dendritic cells from precursor cells such as monocytes. Murine IL-4 acts synergistically with other factors, including GM-CSF (granulocyte-macrophage colony stimulating factor), to drive the differentiation process. Typically, monocytes are cultured in the presence of recombinant IL-4 and GM-CSF to yield immature dendritic cells, which can then be futher matured by additional stimuli.
The package contains the following two vials:
Animal-Free Recombinant Mouse IL-4 Protein（Cat. No. rp154228）
Animal-Free Recombinant Mouse GM-CSF Protein （Cat. No. rp154039）
IL-4 is a pleiotropic cytokine that regulates diverse T- and B-cell responses including cell proliferation, survival, and gene expression. Produced by mast cells, T cells, and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells.
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells.
Components：
A1511077
| Components | Appearance | Size 1 | Size 2 | Storage | Quantity Per Test |
| A1511077A | Animal-Free Recombinant Mouse IL-4 Protein | Lyophilized | 10 μg | 50 μg | -20°C | Use as per requirements |
| A1511077B | Animal-Free Recombinant Mouse GM-CSF Protein | Lyophilized | 10 μg | 50 μg | -20°C | Use as per requirements |
Information:
| Name | Species
| Expression System
| Tag | Purity
| Activity
| Endotoxin
| Appearance
| Storage
| Reconstitution
|
Animal-Free Recombinant Mouse IL-4 Protein
| Mouse | HEK293 | C-His | ≥95% (SDS-PAGE) | Measured in a cell proliferation assay using assay using CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is typically <1.8ng/mL. | <1.0 EU/μg | Lyophilized | Lyophilized from 10mM PBS, pH 7.4 | Reconstitute in sterile H2O at not less than 100µg/ml, which can then be further diluted in other aqueous solutions. |
Animal-Free Recombinant Mouse GM-CSF Protein
| Mouse
| 293F | N-His | ≥95% (SDS-PAGE) | Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 0.04-0.17 ng/mL, corresponding to a specific activity of 5.88×10^6~2.5×10^7 units/mg. | <0.1 EU/μg | Lyophilized | Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4. | Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml. |
Notes:
1. This product is for research use only.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
